Field 2 – Advanced technologies for Personalised Oncology
This field will provide the cancer research community with access to cutting-edge services and technologies, including biological and biomedical imaging, structural biology methods, genomics and analytical pipelines, as well as the associated expertise in their use, to address cancer research questions at a molecular, cellular and whole organism level. The technologies and services offered are closely interlinked with the services and tools in field 1, 3 and 4. This close interlink and collaboration will provide researchers with the opportunity to build service pipelines combining access to advanced technologies for using and analysing cancer models provided by field 1, as well as the tools and technologies needed in their search and validation of new biomarkers (field 3) and new treatments (field 4).
Unique ID | Country | Service Name | Description of Service | Research Infrastructure | Service Provider | Unit of acces | Website |
---|---|---|---|---|---|---|---|
2.29d_BRFAA | GR | Tissue slice culture | Fresh solid tumors from humans or mice are embedded in agarose and sectioned at 150-300 um using a Compresstome® tissue slicer. Slices can be grown in appropriate media for up to one week in Transwell inserts. They can be used for drug testing, immune cell co-cultures, migration studies and other applications. | BBMRI-ERIC | Biomedical Research Foundation of the Academy of Athens | sample | https://www.bioacademy.gr |
2.23_DNS_Uo Lubeck | DE | Image Cytometry | Genomic instability assessment by nuclear DNA ploidy measurements by means of image cytometry using Feulgen stained cytospins or imprints. At least 500 nuclei per sample are selected interactively and the DNA content is measured quantitatively using the ACAS imaging system (Ahrens ACAS, Germany). The DNA profiles are classified according to Auer. | BBMRI-ERIC | Section for Translational Surgical Oncology & Biobanking, Department of Surgery | Sample | https://www.uni-luebeck.de/ncch/lion.html |
2.19e_UZL-qMS | DE | Quantatitve mass spectrometry | Semi-quantitative data-independent mass spectrometry (DIA) making use of a state-of-the-art hybrid trapped ion mobility spectrometry - quadrupole time of flight (TOF) mass spectrometer in parallel accumulation-serial fragmentation (PASEF) mode coupled to nano-flow liquid chromatography. Mass spectrometric raw data are analyzed with the DIA-NN workflow and the library-free mode. | BBMRI-ERIC | Section for Translational Surgical Oncology & Biobanking, Department of Surgery | sample | https://www.uni-luebeck.de/ncch/lion.html |
2.12c_IRST IRCCS | IT | Confocal Microscope | Multimodal advanced light microscopy and super-resolution microscopy, including live cell imaging and functional imaging for characterisation and validation of disease models and confocal microscopy. | BBMRI-ERIC | Istituto Scientifico Romagnolo per lo Studio dei Tumori "Dino Amatori" IRST IRCCS | cell lines | https://www.irst.emr.it |
2.18l_IRST IRCCS | IT | Whole genome sequencing | Whole genome sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologies | BBMRI-ERIC | Istituto Scientifico Romagnolo per lo Studio dei Tumori "Dino Amatori" IRST IRCCS | sample | https://www.irst.emr.it |
2.18m_nCounter_IRST | IT | Target RNA analysis | Target RNA analysis (up to 800 targets per assay) using the nCounter Nanostring platform | BBMRI-ERIC | Istituto Scientifico Romagnolo per lo Studio dei Tumori "Dino Amatori" IRST IRCCS | sample | https://www.irst.emr.it |
2.3a_CliniMacs_IRST | IT | ATMPs Production | Cell Factory for ATMPs production of nanoparticles for RNA delivery | BBMRI-ERIC | Istituto Scientifico Romagnolo per lo Studio dei Tumori "Dino Amatori" IRST IRCCS | Nanoparticle | https://www.irst.emr.it |
2.2b_UNIMIB Med | IT | Laser Dissection | Digital pathology and Machine Learning -based image analysis; quality-controlled digitized whole-slide histological images of tumour and parallel normal tissue including annotated pathological and survival data | BBMRI-ERIC | UNIMIB_Pathology | sample | https://en.unimib.it/ |
2.2c_UNIMIB | IT | Digital Pathology | Dissector with laser acquisition of specific single cells or tissue areas for different uses, including omics | BBMRI-ERIC | UNIMIB_Laser Dissection | sample | https://en.unimib.it/ |
2.19c+.24c_UNIMIB Med | IT | Proteomic_Metabolomic | Proteomics - MS/MS and MALDI Imaging Platform; Quantitative MS, quatitative Mass Spectrometry, Lipidomics and Metabolomics | BBMRI-ERIC | UNIMIB_Proteomic_Metabolomic | Sample | https://en.unimib.it/ |
2.18k | IT | Whole genome sequencing | Whole genome sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologies | BBMRI-ERIC | UNIMIB_Genomic | cell | https://en.unimib.it/ |
2.21a_UNIMIB | IT | Nanomedicine | Development of devices for: a) more efficient intracellular and controlled release of drugs, in particular co-delivery of antigens with immunostimulatory adjuvants , b) protection of antigen payloads and other bioactive molecules, c) increasing antigen bioavailability by encapsulation, adsorption or surface functionalization; d) decreased toxicity; efficient lymphatic drainage, enhanced permeability and retention effect at the tumour site | BBMRI-ERIC | UNIMIB_Nanomedicine | samples | https://en.unimib.it/ |
2.18u_Single cell _LIED | DE | Single cell mRNA sequencing | Single cell mRNA sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologies | BBMRI-ERIC | Lübeck Institute of Experimental Dermatology | sample | https://www.lied.uni-luebeck.de/home.html |
2.18j_CNAG | ES | Whole genome sequencing | Whole genome sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologies | BBMRI-ERIC | Centro Nacional de Análisis Genómico | 1Gbsequence data | https://www.cnag.crg.eu/ |
2.18i_AOU-BO-MSS | IT | Target DNA/RNA sequencing: Oncomine Focus assay | Target DNA and RNA sequencing of hot spot genes, copy number gene variation and gene fusion (Oncomine Focus assay) of tumoral/non tumoral samples using next generation sequencing technologies | BBMRI-ERIC | IRCCS Azienda Ospedaliero Universitaria di Bologna | Sample | https://www.aosp.bo.it/ |
2.18h_AOU-BO-MASS | IT | Target DNA Sequencing: custom gene panels | Target sequencing of custom gene panels (hotspot genes) in tumoral and non tumoral samples using next generation sequencing technologies | BBMRI-ERIC | IRCCS Azienda Ospedaliero Universitaria di Bologna | Sample | https://www.aosp.bo.it/ |
2.12b_BL2_NIL | PL | Confocal Imaging | 2D/3D Imaging of living or fixed stained specimen by confocal microscopy equipped with 488, 543, 633 nm lasers, 4-100x objectives. | BBMRI-ERIC | Department of Biomedical Research / Translational Research Laboratory | Cell line | https://www.nil.gov.pl |
2.35a_BL2_NIL | PL | Molecular Modeling - Docking | Binding analysis of compounds to specific proteins to understand anticancer molecular mechanisms of action | BBMRI-ERIC | Department of Biomedical Research / Translational Research Laboratory | project | https://www.nil.gov.pl |
2.35b_BL2_NIL | PL | Molecular Modeling - Dynamics | LIgand-protein interactions and conformational analysis of proteins upon ligand binding | BBMRI-ERIC | Department of Biomedical Research / Translational Research Laboratory | project | https://www.nil.gov.pl |
2.35c_BL2_NIL | PL | Molecular Modelingt - ab inito | ADME (Absorption, Distribution, Metabolism, and Excretion) properties of tested compounds and evaluation of the conformational stability of molecules | BBMRI-ERIC | Department of Biomedical Research / Translational Research Laboratory | projects | https://www.nil.gov.pl |
2.28a_NMI | PL | Flow Cytometry | Flow cytometry technique (FACSCanto II flow cytometer) to detect apoptotic cells and mitochondrial membrane potential . Cell cycle analysis can be conducted. | BBMRI-ERIC | Department of Biomedical Research / Translational Research Laboratory | Cell | https://www.nil.gov.pl |
2.17a_imag. radiomic_ATMA | IT | Radiomics | Prospective collection of diagnostic images (MR, CT, PT) of cancer patients and advanced imaging analysis (MR, CT, PT) | BBMRI-ERIC | ATMA Expert Center | diagnostic image | |
2.17b_imag. radiomics_ATMA | IT | Radiomics | Advanced imaging analysis for early diagnosis and treatment response. It includes radiomic analysis and features selection to develop oncological personalized models. | BBMRI-ERIC | ATMA Expert Center | diagnostics image | |
2.18o_UNIBO-Microbiome Unit | IT | 16S rRNA microbiome sequencing | Microbial profiling using 16S ribosomal RNA (rRNA) next generation sequencing technologies | BBMRI-ERIC | UNIBO-Microbiome Unit | sample | https://dimec.unibo.it/it |
2.18p_UNIBO Microbiome Unit | IT | Metagenomic microbiome sequencing | Microbial profiling using shotgun metagenomic NGS technologies | BBMRI-ERIC | UNIBO-Microbiome Unit | sample | https://dimec.unibo.it/it |
2.18n_UNIBO Microbiome Unit | IT | Metatranscriptomic microbiome sequencing | Microbial profiling using shotgun metatranscriptomic next generation sequencing technologies | BBMRI-ERIC | UNIBO-Microbiome Unit | sample | https://dimec.unibo.it/it |
2.28b_LTTA-FE | IT | Flow Cytometry | Multiparametric immunophenotyping based on the use of specific fluorescent antibodies to measure targeted proteins expression within a cell population by flow cytometer, to investigate cancer severity, clonal expansion, proliferation, survival and differentiation in response to specific treatments of cancerous cells. Phenotypic characterization of the labeled cells as well as their sorting is available for further investigation. | BBMRI-ERIC | Laboratorio per le Tecnologie delle Terapie Avanzate | sample | https://www.ltta.tecnopoloferrara.it |
2.19b_LTTA-FE | IT | Luminex multiplexing | Cytokines/chemochines quantitative multiparametric analysis, important for understanding the role of inflammation in cancer diseases. Luminex technology permits rapid and accurate analysis of panels of analytes (up to 50 at a time) in small sample volumes (25 µl) of serum/plasma or culture media. | BBMRI-ERIC | Laboratorio per le Tecnologie delle Terapie Avanzate | project | https://www.ltta.tecnopoloferrara.it |
2.18f_UL Biobank | PL | Whole Genome Sequencing | Whole genome sequencing in tumoral and non tumoral samples using NGS technologies | BBMRI-ERIC | University of Lodz - Biobank Lodz | 1 Gb of sequencing data | https://www.biobank.uni.lodz.pl |
2.18g_PanCan NGS | IT | Target DNA sequencing: actionable genes | Target sequencing of actionable genes in tumoral and non tumoral samples using next generation sequencing technologies | BBMRI-ERIC | University Verona | sample | https://www.univr.it |
2.30a_Neuro.path | AT | Chemosensitivity assays | Chemosensitivity testing of tumor cells after treatment, helping to find best suitable drugs for respective patients. Proper concentrations of drugs are used, in order to make the results transferable for clinical use. Establishment of molecular tumor board is supported by introducing this technology. | BBMRI-ERIC | Institut of Pathology, Neuropathology and Molecular Pathology | sample | https://www.i-med.ac.at/research-report/NeuroMolPathology.html |
2.12a_Neuro.path | AT | Histology, Immunohistochemistry, Immunofluorescence, Cytology, Molecular pathology, diagnostics, confocal microscopy | Histological assessment of respective samples, immunohistochemical and immunofluorescence staining for different biomarkers, confocal microscopy, morphometrics; morphological assessment support by A.I. tools available. | BBMRI-ERIC | Institut of Pathology, Neuropathology and Molecular Pathology | sample | https://www.i-med.ac.at/research-report/NeuroMolPathology.html |
2.18d_NCI-LT-Seq-DM | LT | Target DNA sequencing: custom gene panels | Target sequencing of custom gene panels in tumoral and non tumoral samples using Next Generation Sequencing technologies | BBMRI-ERIC | Laboratory of Genetic Diagnostic | sample | https://www.nvi.lt/laboratory-of-genetic-diagnostic/ |
2.18a_Pannoramic MIDI | CZ | Digital Pathology | Whole-slide histological images of tumor and parallel normal tissues that are stored in Bank of Biological Materials including basic histopathological data | BBMRI-ERIC | Department of Pathology | sample | https://www.mou.cz/en/ |
2.18c_Multipotentiostat | CZ | Electrochemical biosensors | Construction of electrochemical biosensors for detection of DNA and/or RNA biomarkers: e.g. detection of HPV (genotyping HPV 16 and 18), SNPs, DNA methylations, miRNAs, lncRNA etc. | BBMRI-ERIC | RECAMO | sample | https://www.mou.cz/en/ |
2.2a_Droplet Dig. PCR | CZ | Digital PCR | Detection of tumour biomarkers (miRNA, DNA mutations, CNVs) in tumour biopsies, cell free DNA, circulating tumor cells, etc. | BBMRI-ERIC | RECAMO | sample | https://www.mou.cz/en/ |
2.18b_NextSeq 500 | CZ | Target DNA sequencing: custom gene panels | Target sequencing of custom gene panels in tumoral and non tumoral samples using next generation sequencing technologies | BBMRI-ERIC | RECAMO | sample | https://www.mou.cz/en/ |
2.18q_BRFAA | GR | Target DNA sequencing: custom gene panels | Target sequencing of custom gene panels in tumoral and non tumoral samples using next generation sequencing technologies | BBMRI-ERIC | Biomedical Research Foundation of the Academy of Athens | sample | http://www.bioacademy.gr/ |
2.18r_BRFAA | GR | Target DNA sequencing: hereditary cancer | Target sequencing of genes involved in hereditary cancer using next generation sequencing technologies | BBMRI-ERIC | Biomedical Research Foundation of the Academy of Athens | sample | http://www.bioacademy.gr/ |
2.18s_BRFAA | GR | Target DNA sequencing: hematological malignancies | Target sequencing of genes involved in hematological malignancies using next generation sequencing technologies | BBMRI-ERIC | Biomedical Research Foundation of the Academy of Athens | sample | http://www.bioacademy.gr/ |
2.18t_BRFAA | GR | Target DNA sequencing: TruSight Oncology 500 | Target sequencing of >500 genes relevant for solid tumors using next generation sequencing technologies | BBMRI-ERIC | Biomedical Research Foundation of the Academy of Athens | sample | http://www.bioacademy.gr/ |
2.16a_BRFAA | GR | PET-CT in mice | Preclinical PET/CT imaging with a state-of-the-art microPET/CT scanner and sophisticated image processing software for analyzing and quantifying PET and CT images of mice and rats. Appropriate racks for hosting animals for a long period of time are available. For more info please visit: https://www.infrafrontier.gr/whole-body-imaging-petct | BBMRI-ERIC | Biomedical Research Foundation of the Academy of Athens | sample | http://www.bioacademy.gr/ |
2.16b_BRFAA | GR | PET-CT and exome-seq in mice | Preclinical PET-CT imaging and exome-sequencing in 1 mouse to reveal genetic variants that alter protein sequences. The Greek Genome Center is equipped with state-of-the-art Illumina sequencers, automated high throughput DNA/RNA extraction and liquid handling workstations and, finally, a high-performance computing cluster. | BBMRI-ERIC | Biomedical Research Foundation of the Academy of Athens | sample | http://www.bioacademy.gr/ |
2.19d_BRFAA | GR | CyTOF analysis | BRFAA mass cytometry unit provides a comprehensive full service, including data analysis, to evaluate cellular phenotype of human and mouse samples including: extracellular and intracellular markers of cell identity, cytokine production, signaling phenotypes and cellular division. We employ multiparametric panels of antigenic markers (>20 markers) with pre-made kits or customizable ones. Examples of such panels can be found at website https://www.brfaacytoflab.com/other-resources. | BBMRI-ERIC | Biomedical Research Foundation of the Academy of Athens | sample | http://www.bioacademy.gr/ |
2.1a_IVM_CNR | IT | Intravital microscopy (IVM) with fluorescent biosensors in mouse models of cancer | Intravital microscopy (IVM) is the study of localization and dynamics of cellular processes relevant to cancer within the native tumor microenvironment. Services include housing of mice, surgical procedures necessary for installation of optical imaging windows adjacent to an tumors/organ of interest. Cancer model may include orthotopic/ectopic xenograft models in immunodeficient, syngeneic mouse tumor cell lines that can be genetically modified to express fluorescent reporters proteins for imaging purposes and genetic mouse models engineered to develop cancer. | INFRAFRONTIER | Consiglio Nazionale delle Ricerche | projects | http://www.ibbc.cnr.it |
2.1b_Fluorescent biosensors | FI | Intravital microscopy (IVM) with fluorescent biosensors in mouse models of cancer | Intravital microscopy (IVM) is the study of localization and dynamics of cellular processes relevant to cancer within the native tumor microenvironment. Services include housing of mice, surgical procedures necessary for installation of optical imaging windows adjacent to an tumors/organ of interest. Cancer model may include orthotopic/ectopic xenograft models in immunodeficient, syngeneic mouse tumor cell lines that can be genetically modified to express fluorescent reporters proteins for imaging purposes and genetic mouse models engineered to develop cancer. | INFRAFRONTIER | University of Oulu | projects | https://www.oulu.fi/en/university/faculties-and-units/biocenter-oulu |
2.2_Dig. path_Oulu | FI | Digital pathology and ML-based image analysis | This service offers quality-controlled digitized whole-slide histological images of tumour and parallel normal tissue, including annotated pathological and survival data and dissector with laser acquisition. | INFRAFRONTIER | University of Oulu | projects | https://www.oulu.fi/en/university/faculties-and-units/biocenter-oulu |
2.1b_ISS Core | IT | Imaging evaluation of murine model during cancer development and treatment | Advanced and quantitative in vivo MRI and MRS to characterize rodents models of cancer during cancer development and following treatment. In particular: DWI with different diffusion models for functional characterization, molecular imaging with iron oxide or 19F nanoparticles for macrofages recruitment studies and quantitative 1H, 31P and 19F MRS for cancer model metabolic characterization. | EATRIS | Istituto Superiore di Sanità (ISS) | MRI and MRS analyses | https://www.iss.it/ |
2.28_Sort FCM | IT | Cell sorting of target population | Cell sorting of target population (e.g. rare population,..), identified by flow or mass cytometry in human tissues | EATRIS | Istituto Superiore di Sanità (ISS) | hour | https://www.iss.it/ |
2.49_Alonso MJ | ES | Immunocompetent murine models of cancer for antitumoral efficacy evaluation | Evaluation of immunocompetent murine models of cancer for antitumoral efficacy, including effects on the tumor microenvironment. Expertise in (1) subcutaneous murine models of any type of cancer; (2) orthotopic immunocompetent models of fibrosarcoma or lung cancer, presenting a high infiltration of tumor-associated macrophages (i.e. MN/MCA or CMT167). | EATRIS | Health Research Institute of Santiago de Compostela (IDIS) | Immunocompetent murine models | https://www.idisantiago.es/ |
2.50_Mol.Biol of Cancer | CZ | Monochrome Multiplex Real-Time qPCR Assay for telomere measurement | Monochrome Multiplex Real-Time qPCR Assay for telomere measurement affected by incomplete DNA replication of a lagging strand or by genotoxic effect of environmental and intracellular DNA-damaging agents, including anticancer drugs. | EATRIS | Institute of Experimental Medicine CAS | experiment/set of 100 samples | http://www.iem.cas.cz/en/institute/ |
2.26_Mol.Boiol of Cancer | CZ | Comet assay (single-cell gel electrophoresis) for measuring DNA strand breaks and oxidative DNA damage | The comet assay (single-cell gel electrophoresis) is a simple method for measuring DNA strand breaks and oxidative DNA damage (Opattova, Horak et al. 2019). This method is applicable for human biomonitoring, testing novel chemicals for genotoxicity, environmental contamination with genotoxins and molecular epidemiology. | EATRIS | Institute of Experimental Medicine CAS | experiment/set of 20 samples | http://www.iem.cas.cz/en/institute/ |
2.48_SDN | IT | Assays for in vitro discovery research | Cell cycle, apoptosis, proliferation and phosflow assay for in vitro discovery research (i.e. drug discovery, pathway analysis) | EATRIS | NETWORK OF NEUROSCIENCE AND NEUROREHABILITATION IRCCS | sample | https://www.reteneuroscienze.it/en/Istituto/irccs-sdn-istituto-di-ricerca/ |
2.16_Mol. imag. biomarkers | ES | microPET/CT acquisition dynamic on murine model | The service includes model generation (if possible in-house), animal handling, dynamic image acquisition and image post-processing. The radiotracer cost is included for radiotracers synthesized at our cyclotron or radiopharmacy facilities (18F-FDG, 18F-FLT, 18F-FMISO, 18F-choline,18F-DOPA, 68Ga-PSMA, 68Ga-DOTATOC). Unit of access includes animal housing and handling, radiotracer, and image processing after the experiment. Deliverables are: dynamic PET/CT images, full quantitative analysis report | EATRIS | Health Research Institute of Santiago de Compostela (IDIS) | 1 animal (sample) | https://www.idisantiago.es/ |
2.11_Molecul. imag. Biomarkers | ES | mAbs labelling: Labelling of novel mAbs with 89Zr for PET imaging. | Labelling of novel mAbs with 89Zr for PET imaging. | EATRIS | Health Research Institute of Santiago de Compostela (IDIS) | mAbs labelling | https://www.idisantiago.es/ |
2.19a_IBET | PT | Advanced Mass Spectrometry for the Characterization of Molecular Tools | The service includes Protein/peptide identification, Antibody Characterization using Multi-Attribute Method (MAM), Post-Translational Modifications Analysis (Glycosylation; Oxidation/Deamination, Glycan Identity…), High-throughput Quantitative Proteomics – SWATH analysis (untargeted; relative quantification), Quantitative Proteomics/peptidomics - MRM-HR (targeted) (relative and/or absolute quantitation) and Intact Protein Measurement. | EATRIS | iBET - Instituto de Biologia Experimental e Tecnológica | Samples+Controls+Standards | https://www.ibet.pt/ |
2.19b_IBET | PT | Characterization of Molecular Tools - Aggregation and refolding (SEC and RP chromatography) | Reverse phase (RP) and Size Exclusion Chromatography (SEC) to assess information on identity and purity attributes of protein-based biopharmaceuticals. SEC is applied for analysis of monomers, dimers, aggregates and degradation products according to their size. RP contributes to characterization of the product in terms of hydrophobicity. | EATRIS | iBET - Instituto de Biologia Experimental e Tecnológica | Sample (2 runs) + dedicated column | https://www.ibet.pt/ |
2.15b+2.46b_Bruker | AT | Preclinical Magnetic Resonance Imaging | Magnetic resonance imaging (MRI) of animal models, comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level. Used to monitor disease development and to test the efficacy of therapeutic treatments, to develop novel diagnostic tools etc. The service includes animal model preparation, provision of contrast agents, image data processing and analysis. | EURO-BIOIMAGING | Austrian BioImaging | hour | https://www.eurobioimaging.eu/nodes/austrian-bioimagingcmi |
2.15c+2.46c_Vevo US | AT | Preclinical UltraSound imaging | UltraSound Imaging of animal models for visualizing and quantifying tumors, allowing staging, monitoring of therapeutic treatments, or guided injection of drugs. The use of targeting microbubbles as US contrast agents allows the visualization and quantification of specific tumor biomarkers as well. The service includes animal model preparation, probes, image data processing and analysis. | EURO-BIOIMAGING | Austrian BioImaging | hour | https://www.eurobioimaging.eu/nodes/austrian-bioimagingcmi |
2.12a+2.33a+2.40a_live cell | BG | Live cell fluorescent imaging | Fluorescence microscopy imaging provided, both in spinning disk and confocal mode, on living cells. This also includes functional imaging to understand protein behavior. The facility specialises in tracking fast protein dynamics and interactions, particularly in DNA repair. | EURO-BIOIMAGING | Advanced Light Microscopy Node Sofia Bulgaria | 1 user project | https://www.eurobioimaging.eu/nodes/sofia-bioimaging-node---advanced-light-microscopy-node-sofia-bulgaria |
2.12b+2.33b+2.40b_LM Super | CZ | LM Super-resolution and confocal microscopy | A variety of different light microscopy methods are available individually or in combination. These methods include functional imaging with fluorescence microscopy, a particular focus on super-resolution imaging using specifcially STED and SIM to reach higher resolution compared to what is available using standard methods. These methods also include mesoscopic imaging via lightsheet, high-speed imaging, second-harmonics generation and other specialised light microscopy methods | EURO-BIOIMAGING | Advanced Light and Electron Microscopy Node Prague CZ | Hour | https://www.eurobioimaging.eu/nodes/advanced-light-and-electron-microscopy-node-prague-cz |
2.42d_LM Live Cell | CZ | LM Live cell QPI | Quantitative Phase Imaging of living cells is a label-free light microscopy method to retrieve the phase information of light passing through the cell, and via this determine dry mass of the cell, its changes over time, and track cellular behavior over time. Due to the label free nature of the imaging, it can be applied to unstained samples, including patient biopsies. It is a gentle imaging method with little photodamage and can be applied to living cells over time. | EURO-BIOIMAGING | Advanced Light and Electron Microscopy Node Prague CZ | hour | https://www.eurobioimaging.eu/nodes/advanced-light-and-electron-microscopy-node-prague-cz |
2.14b_EM 3D Methods | CZ | EM 3D methods | Volume EM methods are used to gain insights into ultrastructural organisation of biological samples at a sub-cellular level over a 3D volume of tissue. This can be applied to different tissues samples using various sample preparation methods. Specifically offered volume EM methods are serial-section TEM, serial blockface SEM, and FIB-SEM (Focussed Ion Beam Scanning Electron Microscopy). | EURO-BIOIMAGING | Advanced Light and Electron Microscopy Node Prague CZ | hour | https://www.eurobioimaging.eu/nodes/advanced-light-and-electron-microscopy-node-prague-cz |
2.14c_EM Routine | CZ | EM routine TEM | Standard transmission electron microscopy methods to gain ultrastructural insights into subcellular organisation. The service includes different sample preparation methods, such as cryo-EM and resin-EM, and a variety of labelling methods to localise specific molecules within a sample. | EURO-BIOIMAGING | Advanced Light and Electron Microscopy Node Prague CZ | hour | https://www.eurobioimaging.eu/nodes/advanced-light-and-electron-microscopy-node-prague-cz |
2.12c+2.33c+2.40c_CELLIM | CZ | Fluorescence Microscopy | Service provides access and support for a variety of light microscopy methods, specifically functional imaging with fluorescence microscopy, super-resolution imaging via single molecule localisation microscopy (SMLM) and structured illumination microscopy (SIM), mesoscopic imaging via lightsheet, expansion microscopy and other specialised light microscopy methods | EURO-BIOIMAGING | Advanced Light Microscopy and Medical Imaging Node Brno CZ | hour | https://www.eurobioimaging.eu/nodes/advanced-light-microscopy-and-medical-imaging-node-brno-cz |
2.15d+2.46d_Albira | CZ | Preclinical PET-CT and SPECT-CT imaging | Positron Emission Tomography (PET) and Single Photon Emission Tomography (SPECT) Imaging of animal models, for monitoring function and metabolism. PET and SPECT imaging can be performed as single modality acquisition but can also be used in combination with CT, for precise spatial localization of the PET/SPECT signals within the subject. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. | EURO-BIOIMAGING | Centre for Advanced Preclinical Imaging | hour | https://www.eurobioimaging.eu/nodes/center-for-advanced-preclinical-imaging-capi |
2.15e+2.46e_Xtreme Optical | CZ | In vivo optical imaging | In vivo preclinical optical Imaging, for visualizing and characterizing tumors and for imaging guided surgery. Fluorescence, bioluminescence and other optical imaging techniques are available. A variety of bioluminescent and/or fluorescent probes can be used, each targeted to a specific tissue or molecular or cellular event. Animal model preparation, probes and image data processing and analysis are included in the service. | EURO-BIOIMAGING | Centre for Advanced Preclinical Imaging | hour | https://www.eurobioimaging.eu/nodes/center-for-advanced-preclinical-imaging-capi |
2.15f+2.46f_VEVO US | CZ | Preclinical UltraSound imaging | UltraSound Imaging of animal models for visualizing and quantifying tumors, allowing staging, monitoring of therapeutic treatments, or guided injection of drugs. The use of targeting microbubbles as US contrast agents allows the visualization and quantification of specific tumor biomarkers as well. The service includes animal model preparation, probes, image data processing and analysis. | EURO-BIOIMAGING | Centre for Advanced Preclinical Imaging | hour | https://www.eurobioimaging.eu/nodes/center-for-advanced-preclinical-imaging-capi |
2.15g+2.46g_7T MRI | CZ | Preclinical Magnetic Resonance Imaging | Magnetic resonance imaging (MRI) of animal models, comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level. Used to monitor disease development and to test the efficacy of therapeutic treatments, to develop novel diagnostic tools etc. The service includes animal model preparation, provision of contrast agents (with possibility of custom synthesis of specific targeting and/or theranostic probes if needed), image data processing and analysis. | EURO-BIOIMAGING | Centre for Advanced Preclinical Imaging | complete access | https://www.eurobioimaging.eu/nodes/center-for-advanced-preclinical-imaging-capi |
2.12d+2.33d+2.40d+2.13a+2.41a_EMBL | DE | Fluorescence Microscopy | Different fluorescence microscopy methods are offered including expertise in project development and image analysis. The offered methods include advanced super-resolution microscopy techniques such as single molecule localisation, STED, MINFLUX, live cell imaging, mesoscopic imaging via lightsheet, functional imaging, intravital microscopy, high-throughput imaging and correlative techniques. | EURO-BIOIMAGING | EMBL-Node | hour | https://www.eurobioimaging.eu/nodes/advanced-light-microscopy-facility-embl |
2.14d_EMBL | DE | Electron Microscopy | Different electron microscopy methods are offered, including correlative light and electron microscopy methods (CLEM), a variety of sample preparations, including cryo-EM methods, labelling methods to localise proteins within EM samples, and volume EM methods for 3D ultrastructural investigations. VolumeEM methods offered are serial section TEM, Serial Blockface-SEM, FIB-SEM | EURO-BIOIMAGING | EMBL-Node | hour | https://www.eurobioimaging.eu/nodes/advanced-light-microscopy-facility-embl |
2.12f+2.33f+2.40f_Super-resolution mic. | FI | Super-resolution Microscopy | Super-resolution methods offered to gain increased resolution insights using fluorescence microscopy beyond what is gathered using standard fluorescence methods. The offered super-resolution approaches are single-molecule localisation microscopy (PALM, STORM), structured illumination microscopy (SIM), and STED, including needed sample preparation and analysis steps. | EURO-BIOIMAGING | Finnish Advanced Microscopy Node | hour | https://www.eurobioimaging.eu/nodes/finnish-alm-node---advanced-light-microscopy-finnish-node |
2.14e_Electron Mocro. | FI | Electron Microscopy | Different electron microscopy methods are offered, including labelling methods to localise proteins within EM samples, and volume EM methods for 3D ultrastructural investigations. VolumeEM methods offered are Array tomography, serial section TEM, Serial Blockface-SEM. | EURO-BIOIMAGING | Finnish Advanced Microscopy Node | hour | https://www.eurobioimaging.eu/nodes/finnish-alm-node---advanced-light-microscopy-finnish-node |
2.14f_CLEM | FI | Correlative Light and Electron Microscopy | Correlative Light and Electron Microscopy combines and correlates light and electron microscopy imaging of the same sample to achieve a higher level of understanding of the sample than possible with either technique in isolation. The facilities offer expertise starting from imaging of living cells using fluorescence microscopy, up to correlation with different EM methods for ultrastructural localisation of correlated proteins of interest. | EURO-BIOIMAGING | Finnish Advanced Microscopy Node | hour | https://www.eurobioimaging.eu/nodes/finnish-alm-node---advanced-light-microscopy-finnish-node |
2.13b+2.41b_Mesoscopic Imaging | FI | Mesoscopic Imaging | Mesoscopic Imaging allows imaging of larger samples, such as tissues, organoids, and small animals at high resolution. This can be combined with cleared and expanded tissues. The specific offered methods are lightsheet microscopy and optical projection tomography | EURO-BIOIMAGING | Finnish Advanced Microscopy Node | hour | https://www.eurobioimaging.eu/nodes/finnish-alm-node---advanced-light-microscopy-finnish-node |
2.15h+2.46h_Fibl-MEG | FI | MagnetoEncephaloGraphy & MRI | Magnetoencephalography (MEG) measures noninvasively minute magnetic fields produced by neuronal activity of the brain. MEG (as a standalone technique or coupled with MRI) can be used for mapping functionally important regions in the brain prior to surgery and for detecting functional impairment caused by brain tumors. | EURO-BIOIMAGING | Finnish BioMedical Imaging Node | complete access | https://www.eurobioimaging.eu/nodes/finnish-biomedical-imaging-node |
2.17a_PET/CT | FI | Preclinical PET-CT Imaging | Positron Emission Tomography (PET) Imaging of animal models, for monitoring function and metabolism. PET imaging can be performed as single modality acquisition but can also be used in combination with CT, for precise spatial localization of the PET signals within the subject. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. | EURO-BIOIMAGING | Finnish BioMedical Imaging Node | complete access | https://www.eurobioimaging.eu/nodes/finnish-biomedical-imaging-node |
2.17b+2.15i+2.46i_SPECT/CT | FI | Preclinical SPECT-CT Imaging | Single Photon Emission Tomography (SPECT) Imaging of animal models, for monitoring function and metabolism. SPECT imaging can be performed as single modality acquisition but can also be used in combination with CT, for precise spatial localization of the SPECT signals within the subject. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. | EURO-BIOIMAGING | Finnish BioMedical Imaging Node | complete access | https://www.eurobioimaging.eu/nodes/finnish-biomedical-imaging-node |
2.12e+2.33e+2.40e_Multimodal | FI | Multimodal Advanced Light Microscopy | Advanced fluorescence microscopy methods include live cell imaging, functional imaging to investigate protein dynamics and interactions (FRET, FRAP, FLIM, FCCS), second harmonics generation, total internal reflection fluorecence microscopy (TIRF), and correlative techniques. | EURO-BIOIMAGING | Finnish Advanced Microscopy Node | hour | https://www.eurobioimaging.eu/nodes/finnish-alm-node---advanced-light-microscopy-finnish-node |
2.12g+2.33g+2.40g_Reg.Light | FR | Regular Light Microscopy | Light Microscopy methods are offered including fluorescence and label-free techniques, functional imaging, confocal and deconvolution microscopy, to investigate cellular and sub-cellular behavior and localisations. These methods can be applied also correlatively with high-end fluorescence microscopy and electron microscopy methods | EURO-BIOIMAGING | French BioImaging Node | hour | https://www.eurobioimaging.eu/nodes/french-bioimaging-node |
2.12h+2.33h+2.40h | FR | High and super-resolution microscopy | Super-resolution methods including single-molecule localisation microscopy (PALM, STORM), SIM, STED, as well as methods for high resolution imaging on standard systems such as image scanning microscopy on confocal and spinning disk systems. These methods improve the resolution for the gathered data from an around 2-fold increase to very high resolution on the nm level. | EURO-BIOIMAGING | French BioImaging Node | hour | https://www.eurobioimaging.eu/nodes/french-bioimaging-node |
2.13c+2.41c_Longitudinal Interval | FR | Longitudinal intravital microscopy | Intravital imaging covers a range of microscopy modalities used for the long term imaging of living animal models (e.g. zebrafish larvae, mice, rats), in various organs or tissues, or as a whole for smaller models. These methods allow tracking of tissue, cellular, and even sub-cellular, behavior in a larger context of either an organ or organoid, or a whole animal. In whole animals this is done using windows, such as cranial windows and abdominal windows, to allow access to the inside of the animal | EURO-BIOIMAGING | French BioImaging Node | hour | https://www.eurobioimaging.eu/nodes/french-bioimaging-node |
2.13d+2.41d_Lattice Light Sheet | FR | Lattice Lightsheet Microscopy | Lattice Light Sheet Microscopy is a special lightsheet microscope where the excitation plane is very thin (<1 µm) and uniformly flat over typical cellular dimensions. The main advantages of LLSM are its intrinsic optical sectioning, fast imaging (up to 300 frames per second), low photobleaching and phototoxicity, and sub-micrometer spatial resolution over long light sheets (10–100 µm). | EURO-BIOIMAGING | French BioImaging Node | hour | https://www.eurobioimaging.eu/nodes/french-bioimaging-node |
2.14g_Reg. EM/TEM | FR | Electron Microscopy | Transmission Electron Microscopy and Scanning Electron Microscopy on samples prepared with different methods, such as resin-EM and cryo-EM. This also includes support for different sample labelling methods to localise proteins in the sample. | EURO-BIOIMAGING | French BioImaging Node | complete access | https://www.eurobioimaging.eu/nodes/french-bioimaging-node |
2.12i+2.33i+2.40i | FR | High-end confocal Microscopy | High-end and specialised confocal microscopy methods to achieve high-resolution and fast imaging with 3D resolution, including in living and fixed cells | EURO-BIOIMAGING | French BioImaging Node | complete access | https://www.eurobioimaging.eu/nodes/french-bioimaging-node |
2.14h_CLEM | FR | Advanced Electron Microscopy | Advanced Electron Microscopy methods, including cryo-EM sample presentation, Correlative Light and Electron Microscopy, and volume EM methods for 3D ultrastructural investigations in a larger cellular and tissue context. The available volumeEM methods are FIB-SEM, serial blockface-SEM, and serial section TEM. This also includes Correlative X-ray, light and electron microscopy imaging | EURO-BIOIMAGING | French BioImaging Node | hour | https://www.eurobioimaging.eu/nodes/french-bioimaging-node |
2.12j+2.33j+2.40j_UNIDEB | HU | Advanced Light Microscopy | Advanced Light Microscopy methods, particularly fluorescence imaging methods with a specific focus on functional imaging methods to study protein interactions and dynamics (FRET, FRAP, FLIM, FCCS), as well as high-throughput imaging and light-sheet microscopy | EURO-BIOIMAGING | Cellular Imaging Hungary Node | complete access | https://www.eurobioimaging.eu/nodes/cellular-imaging-hungary |
2.12k+2.33k+2.40k_Semmelweis | HU | Super-resolution | A range of microscopy techniques used to achieve a resolution that is higher than that of conventional, diffraction-limited microscopy, particularly single-molecule localisation microscopy (SMLM) and STED. | EURO-BIOIMAGING | Cellular Imaging Hungary Node | complet access | https://www.eurobioimaging.eu/nodes/cellular-imaging-hungary |
2.14i_BRC CILAB | HU | Advanced Light and correlative imaging methods | Advanced Light Microscopy methods, including super-resolution and functional imaging, in combination and correlation with Electron Microscopy approaches and methods for sample characterisation. | EURO-BIOIMAGING | Cellular Imaging Hungary Node | complete access | https://www.eurobioimaging.eu/nodes/cellular-imaging-hungary |
2.12l+2.33l+2.40l_FEMTO | HU | Two-photon Microscopy | Multiphoton microscopy - most commonly in the form of two-photon microscopy - is a fluorescence imaging technique that allows observation of living tissue up to about one millimeter in depth. | EURO-BIOIMAGING | Cellular Imaging Hungary Node | complete access | https://www.eurobioimaging.eu/nodes/cellular-imaging-hungary |
2.15j+2.46j | HU | Preclinical PET Imaging and Autoradiography | Positron Emission Tomography (PET) Imaging of animal models, for monitoring function and metabolism. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. | EURO-BIOIMAGING | Medical and Preclinical Imaging Hungary | complete access | https://www.eurobioimaging.eu/nodes/medical-and-preclinical-imaging-hungary |
2.17c_SCANOMED | HU | Preclinical PET-CT and SPECT-CT imaging | Positron Emission Tomography (PET) and Single Photon Emission Tomography (SPECT) Imaging of animal models, for monitoring function and metabolism. PET and SPECT imaging can be performed as single modality acquisition but can also be used in combination with CT, for precise spatial localization of the PET/SPECT signals within the subject. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. | EURO-BIOIMAGING | Medical and Preclinical Imaging Hungary | complete access | https://www.eurobioimaging.eu/nodes/medical-and-preclinical-imaging-hungary |
2.15k+2.46k+2.17d_Semmelweis | HU | Preclinical PET-CT and SPECT-CT imaging | Positron Emission Tomography (PET) and Single Photon Emission Tomography (SPECT) Imaging of animal models, for monitoring function and metabolism. PET and SPECT imaging can be performed as single modality acquisition but can also be used in combination with CT, for precise spatial localization of the PET/SPECT signals within the subject. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. | EURO-BIOIMAGING | Medical and Preclinical Imaging Hungary | complete access | https://www.eurobioimaging.eu/nodes/medical-and-preclinical-imaging-hungary |
2.15l+2.46l_UP,SzRC | HU | In vivo Optical Imaging | In vivo preclinical optical Imaging, for visualizing and characterizing tumors and for imaging guided surgery. Fluorescence, bioluminescence and other optical imaging techniques are available. A variety of bioluminescent and/or fluorescent probes can be used, each targeted to a specific tissue or molecular or cellular event. Animal model preparation, probes and image data processing and analysis are included in the service. | EURO-BIOIMAGING | Medical and Preclinical Imaging Hungary | complete access | https://www.eurobioimaging.eu/nodes/medical-and-preclinical-imaging-hungary |
2.15m+2.46m_Biospec | IL | Preclinical Magnetic Resonance Imaging | Magnetic resonance imaging (MRI) of animal models, comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level. Used to monitor disease development and to test the efficacy of therapeutic treatments, to develop novel diagnostic tools etc. The service includes animal model preparation, provision of contrast agents (with possibility of custom synthesis of specific targeting and/or theranostic probes if needed), image data processing and analysis. | EURO-BIOIMAGING | Israel BioImaging | Shift (4 hours) | https://www.eurobioimaging.eu/nodes/israel-bioimaging |
2.12m+2.33m+2.40m_Ultramicro | IL | Super-resolution | A range of microscopy techniques used to achieve a resolution that is higher than that of conventional, diffraction-limited microscopy, specifically as single-molecule localisation microscopy (SMLM) and STED. | EURO-BIOIMAGING | Israel BioImaging | hour | |
2.19a,2.42a_VBCF | AT | low-resolution Mass Spectrometry based Imaging | Surface analysis techniques which are based on desorption and ionization of molecules followed by their subsequent MS data recording. After collecting a mass spectrum at one spot, the sample is moved to the neighboring spot, and the mass spectrum is sampled again, until the entire sample surface is scanned | EURO-BIOIMAGING | Austrian BioImaging | Hour | https://www.eurobioimaging.eu/nodes/austrian-bioimagingcmi |
2.32a_HCS | IL | High-content screening | Using automated cell manipulations and microscopy platforms, high-content screening can range from entire genomes screens and high-content analysis, to slide scanning of a large number of tissue biopsy sections. HCS techniques rely on fully automated widefield or confocal microscopy systems which facilitate the capture of thousands of images, often in multi-well plates or on slides. | EURO-BIOIMAGING | Israel BioImaging | day | https://www.eurobioimaging.eu/nodes/israel-bioimaging |
2.15n+2.46n_in vivo optic.imag. | IL | In vivo Optical Imaging | In vivo preclinical optical Imaging, for visualizing and characterizing tumors and for imaging guided surgery. Fluorescence, bioluminescence and other optical imaging techniques are available. A variety of bioluminescent and/or fluorescent probes can be used, each targeted to a specific tissue or molecular or cellular event. This service makes use of an Olympus optical system. Animal model preparation, probes and image data processing and analysis are included in the service. | EURO-BIOIMAGING | Israel BioImaging | hour | https://www.eurobioimaging.eu/nodes/israel-bioimaging |
2.15o+2.46o_in vivo optic. imag. | IL | In vivo Optical Imaging | In vivo preclinical optical Imaging, for visualizing and characterizing tumors and for imaging guided surgery. Fluorescence, bioluminescence and other optical imaging techniques are available. A variety of bioluminescent and/or fluorescent probes can be used, each targeted to a specific tissue or molecular or cellular event. This service makes use of a Spektrum IVIS optical system. Animal model preparation, probes and image data processing and analysis are included in the service. | EURO-BIOIMAGING | Israel BioImaging | hour | https://www.eurobioimaging.eu/nodes/israel-bioimaging |
2.15p+2.46p_in vivo optic.imag | IL | In vivo Optical Imaging | In vivo preclinical optical Imaging, for visualizing and characterizing tumors and for imaging guided surgery. Fluorescence, bioluminescence and other optical imaging techniques are available. A variety of bioluminescent and/or fluorescent probes can be used, each targeted to a specific tissue or molecular or cellular event. This service makes use of an IVIS-KINETIC optical system. Animal model preparation, probes and image data processing and analysis are included in the service. | EURO-BIOIMAGING | Israel BioImaging | hour | https://www.eurobioimaging.eu/nodes/israel-bioimaging |
2.12n+2.33n+2.40n_LM | IT | Light Microscopy | Light Microscopy methods are offered including fluorescence and label-free techniques, multiphoton and volumetric imaging, non-linear microscopy, functional imaging for protein dynamics and interactions, in-flow microscopy, large sample imaging in cleared and expanded samples | EURO-BIOIMAGING | Advanced Light Microscopy Italian Node | complete access | https://www.eurobioimaging.eu/nodes/advanced-light-microscopy-italian-node |
2.12o+2.33o+2.40o_SR | IT | Super-resolution Microscopy | Single-molecular localisation microscopy (PALM, STORM) including both in 2D and 3D, STED, and image scanning microscopy to achieve higher resolution images compared to standard diffraction-limited microscopy | EURO-BIOIMAGING | Advanced Light Microscopy Italian Node | complete access | https://www.eurobioimaging.eu/nodes/advanced-light-microscopy-italian-node |
2.14j_EM | IT | Electron Microscopy | Different electron microscopy methods, particularly as CLEM in correlation with light microscopy. The offered methods include transmission, scanning, cryo, and volume EM, such as FIB-SEM and serial section TEM. | EURO-BIOIMAGING | Advanced Light Microscopy Italian Node | complete access | https://www.eurobioimaging.eu/nodes/advanced-light-microscopy-italian-node |
2.32b_HTI | IT | High-throughput Imaging | Using automated cell manipulations and microscopy platforms, high-content screening can range from entire genomes screens and high-content analysis, to slide scanning of a large number of tissue biopsy sections. HCS techniques rely on fully automated widefield or confocal microscopy systems which facilitate the capture of thousands of images, often in multi-well plates or on slides. | EURO-BIOIMAGING | Advanced Light Microscopy Italian Node | complete access | https://www.eurobioimaging.eu/nodes/advanced-light-microscopy-italian-node |
2.15q+2.46q_micro PET | IT | Preclinical PET-CT imaging | Positron Emission Tomography (PET) Imaging of animal models, for monitoring function and metabolism. PET imaging can be performed as single modality acquisition but can also be used in combination with CT, for precise spatial localization of the PET signals within the subject. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. | EURO-BIOIMAGING | Digital Imaging Multimodal Platform Neuromed - DIMP NEUROMED | complete access | https://www.eurobioimaging.eu/nodes/digital-imaging-multimodal-platform-neuromed---dimp-neuromed |
2.19b+2.42b_Brucker FTICR | AT | High-resolution Mass Spectrometry based Imaging | Surface analysis techniques which are based on desorption and ionization of molecules followed by their subsequent MS data recording. After collecting a mass spectrum at one spot, the sample is moved to the neighboring spot, and the mass spectrum is sampled again, until the entire sample surface is scanned | EURO-BIOIMAGING | Austrian BioImaging | Hour | https://www.eurobioimaging.eu/nodes/austrian-bioimagingcmi |
2.12p+2.33p+2.40p | IT | Light Microscopy | Foundational light microscopy tools and sample preparation for immunohistochemistry on animal samples provided in concert with other preclinical imaging measures | EURO-BIOIMAGING | Digital Imaging Multimodal Platform Neuromed - DIMP NEUROMED | complete access | https://www.eurobioimaging.eu/nodes/digital-imaging-multimodal-platform-neuromed---dimp-neuromed |
2.15r+2.46r_Population imag. | IT | Population imaging | Application of advanced image analysis tools to large numbers of medical images related to population cohorts for the identification and monitoring of specific imaging biomarkers which can provide information about diseases at the population level, allowing early diagnosis, early identification of people at risk and preventive measures. | EURO-BIOIMAGING | Digital Imaging Multimodal Platform Neuromed - DIMP NEUROMED | complete access | https://www.eurobioimaging.eu/nodes/digital-imaging-multimodal-platform-neuromed---dimp-neuromed |
2.17e_Phase Contrast | IT | Phase Contrast X-ray Imaging | In respect to other x-rays based imaging techniques, Phase Contrast X-ray Imaging (PCI) is useful for imaging soft tissues that do not possess large variations in X-ray absorption properties. Phase Contrast Imaging can also be combined with tomographic techniques to obtain 3D reconstruction. Image data processing and analysis included in the service. | EURO-BIOIMAGING | Phase Contrast Imaging Flagship Node Trieste | Hour | https://www.eurobioimaging.eu/nodes/phase-contrast-imaging-flagship-node-trieste |
2.15s+2.46s_MRI | IT | Preclinical Magnetic Resonance Imaging | Magnetic resonance imaging (MRI) of animal models, comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level. Used to monitor disease development and to test the efficacy of therapeutic treatments, to develop novel diagnostic tools etc. The service includes animal model preparation, provision of contrast agents (with possibility of custom synthesis of specific targeting and/or theranostic probes if needed), image data processing and analysis. | EURO-BIOIMAGING | MultiModal Molecular Imaging Italian Node | complete access | https://www.eurobioimaging.eu/nodes/molecular-imaging-italian-node |
2.15t+2.46t_PET/SPECT | IT | Preclinical PET-CT and SPECT-CT imaging | Positron Emission Tomography (PET) and Single Photon Emission Tomography (SPECT) Imaging of animal models, for monitoring function and metabolism. PET and SPECT imaging can be performed as single modality acquisition but can also be used in combination with CT, for precise spatial localization of the PET/SPECT signals within the subject. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. | EURO-BIOIMAGING | MultiModal Molecular Imaging Italian Node | complete access | https://www.eurobioimaging.eu/nodes/molecular-imaging-italian-node |
2.17f_CT_MMMI 3 | IT | Preclinical X-Rays Computed Tomography | X-rays Computed Tomography (CT) makes use of X-ray images (projections) taken from different angles and image reconstruction techniques to produce high resolution anatomic cross-sectional (tomographic) 3D images. CT contrast agents (usually based on Iodine, Barium, Gold) can be used to enhance the contrast. Preclinical microCT systems can be stand alone or integrated with other modalities like optical or nuclear medicine imaging in order to perform multimodal imaging. | EURO-BIOIMAGING | MultiModal Molecular Imaging Italian Node | complete access | https://www.eurobioimaging.eu/nodes/molecular-imaging-italian-node |
2.34a | IT | Preclinical Photocoustic imaging | Photoacoustic Imaging (PAI) of animal models, combining the advantages of optical and US imaging technologies. The US signal makes it possible to achieve an increase of both spatial resolution (micrometric) and penetration depth with respect to optical imaging and reduces scattering phenomena. In comparison with US a wider range of molecules can be used as PAI contrast agents. PAI allows to get 3D-high resolution images of soft tissues which provide anatomical, functional, and molecular information. The service includes animal model preparation, probes and image data processing and analysis. | EURO-BIOIMAGING | MultiModal Molecular Imaging Italian Node | complete access | https://www.eurobioimaging.eu/nodes/molecular-imaging-italian-node |
2.14k_Light fluorescence | NL | Light and Fluorescence Microscopy | Light Microscopy methods, including fluorescence microscopy in deconvolution widefield, confocal and spinning disk mode, functional imaging such as FLIM, FRET, FRAP and FCCS to measure protein dynamics and interactions | EURO-BIOIMAGING | Facility of MultiModal Imaging, AMMI Maastricht | hour | https://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht |
2.12q+2.33q+2.40q_Spectral fluor. | NL | Multispectral Imaging | Mulitspectral Imaging allows the simultaneous detection mulitple biomarkers in tissues at the same time within a sample, including when they are co-localised. This is especially applicable for tissue section and biopsies where samples are rare and individual can be simultaneously stained with many markers to extract maximum information from individual samples. | EURO-BIOIMAGING | Facility of MultiModal Imaging, AMMI Maastricht | hour | https://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht |
2.12r+2.33r+2.40r | NL | Super-resolution Microscopy | Super-resolution microscopy, specifically in the form of STED microscopy, for increased resolution compared to standard diffraction-limited microscopy | EURO-BIOIMAGING | Facility of MultiModal Imaging, AMMI Maastricht | hour | https://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht |
2.42c_ATI XRayLab | AT | Micro X-ray Fluorescence Spectrometry | Micro-X-Ray Fluorescence Spectrometry (XRF) allows the qualitative analysis of the elemental composition of solid samples. The detected signal results from the emission of element-characteristic fluorescence X-rays from a sample bombarded with high-energy X-rays | EURO-BIOIMAGING | Facility of MultiModal Imaging, AMMI Maastricht | Week | https://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht |
2.13e+2.41e_Light fluor. | NL | Two-photon microscopy | Multiphoton microscopy - most commonly in the form of two-photon microscopy - is a fluorescence imaging technique that allows observation of living tissue up to about one millimeter in depth. | EURO-BIOIMAGING | Facility of MultiModal Imaging, AMMI Maastricht | hour | https://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht |
2.14l | NL | Transmission Electron Microscopy | In transmission electron microscopy (TEM) the accelerated beam of electrons is transmitted through the sample and collected to form an image. To achieve this the specimen is usually sectioned into ultrathin sections less than 100 nm thick. Different methods for sample preparation are available | EURO-BIOIMAGING | Facility of MultiModal Imaging, AMMI Maastricht | hour | https://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht |
2.14m | NL | Cryo-Transmission Electron Microscopy | In transmission electron microscopy (TEM) the accelerated beam of electrons is transmitted through the sample and collected to form an image. To achieve this the specimen is usually sectioned into ultrathin sections less than 100 nm thick. Here specifically samples are cryo-fixed, either through plunge freezing for very small samples or via high-pressure freezing | EURO-BIOIMAGING | Facility of MultiModal Imaging, AMMI Maastricht | hour | https://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht |
2.14n | NL | Scanning Electron Microscopy | Scanning electron microscopy describes a mode of electron microscopy in which the beam of accelerated electrons is scanned along the surface of the sample to gather insights into the surface structure at nanometer resolution. This can be applied to either whole samples or sections. | EURO-BIOIMAGING | Facility of MultiModal Imaging, AMMI Maastricht | hour | https://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht |
2.14o_DualBeam | NL | Dual Beam Scanning Electron Microscopy | Scanning electron microscope with a dual beam set up allowing for faster data acquisition in scanning electron microscopy mode. | EURO-BIOIMAGING | Facility of MultiModal Imaging, AMMI Maastricht | hour | https://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht |
2.19c+2.42e_SOLARIX | NL | Mass-Spectrometry based Imaging using SOLARIX | Surface analysis techniques which are based on desorption and ionization of molecules followed by their subsequent MS data recording. After collecting a mass spectrum at one spot, the sample is moved to the neighboring spot, and the mass spectrum is sampled again, until the entire sample surface is scanned. Here performed via SOLARIX. | EURO-BIOIMAGING | Facility of MultiModal Imaging, AMMI Maastricht | Hour | https://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht |
2.19d+2.42f_RAPIFLEX | NL | Mass-Spectrometry based Imaging RAPIFLEX | Surface analysis techniques which are based on desorption and ionization of molecules followed by their subsequent MS data recording. After collecting a mass spectrum at one spot, the sample is moved to the neighboring spot, and the mass spectrum is sampled again, until the entire sample surface is scanned. Here performed via SOLARIX. | EURO-BIOIMAGING | Facility of MultiModal Imaging, AMMI Maastricht | Hour | https://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht |
2.32c | NL | Mass-Spectrometry based Imaging using HTX | Surface analysis techniques which are based on desorption and ionization of molecules followed by their subsequent MS data recording. After collecting a mass spectrum at one spot, the sample is moved to the neighboring spot, and the mass spectrum is sampled again, until the entire sample surface is scanned. Here performed via HTX | EURO-BIOIMAGING | Facility of MultiModal Imaging, AMMI Maastricht | hour | https://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht |
2.13f_LEICA | NL | Light Microscopy | Light Microscopy methods, specifcally label-free techniques, to investigate cellular and sub-cellular behavior and localisations | EURO-BIOIMAGING | Facility of MultiModal Imaging, AMMI Maastricht | hour | https://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht |
2.15u+2.46u_PET/SPECT | NL | Preclinical PET and SPECT imaging | Positron Emission Tomography (PET) and Single Photon Emission Tomography (SPECT) Imaging of animal models, for monitoring function and metabolism. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. | EURO-BIOIMAGING | Facility of MultiModal Imaging, AMMI Maastricht | complete access | https://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht |
2.14a_HREM | AT | High-resolution Episcopic microscopy | High-resolution Episcopic microscopy or Serial block face fluorescence imaging is a method used to generate 3-dimensional (3D) reconstruction of a sample via serial image acquisition, here on a microtome, using light microscopy imaging techniques. | EURO-BIOIMAGING | Austrian BioImaging | hour | https://www.eurobioimaging.eu/nodes/austrian-bioimagingcmi |
2.17f_CT | NL | Preclinical X-Rays Computed Tomography | X-rays Computed Tomography (CT) on tissues, organs or living animal models. CT contrast agents (usually based on Iodine, Barium, Gold) can be used to enhance the contrast. Preclinical microCT systems can be stand alone or integrated with other modalities like optical or nuclear medicine imaging in order to perform multimodal imaging. The service includes animal model prepartion, contrast agents if needed, image data processing and analysis. | EURO-BIOIMAGING | Facility of MultiModal Imaging, AMMI Maastricht | complete access | https://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht |
2.15v+2.46v_in vivo optic.imag | NL | In vivo Optical Imaging | In vivo preclinical optical Imaging, for visualizing and characterizing tumors and for imaging guided surgery. Fluorescence, bioluminescence and other optical imaging techniques are available. A variety of bioluminescent and/or fluorescent probes can be used, each targeted to a specific tissue or molecular or cellular event. Animal model preparation, probes and image data processing and analysis are included in the service. | EURO-BIOIMAGING | Facility of MultiModal Imaging, AMMI Maastricht | complete access | https://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht |
2.14p_Electron microsc. | NL | Electron Microscopy Sample preparation support | Support with both the selection of the most appropriate sample preparation technique for different Electron Microscopy investigations, trouble-shooting of protocols and practical sample preparation support | EURO-BIOIMAGING | Facility of MultiModal Imaging, AMMI Maastricht | hour | https://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht |
2.14q_Dutch CLEM | NL | Correlative Light and Electron Microscopy | A wide variety of electron microscopy methods, including correlative light and electron microscopy (CLEM) is available. The EM methods include different vEM modalities, such as Array Tomography, FIB-SEM, serial blockface SEM and serial section TEM. Different methods for sample prepartion inlcuding chemical and cryo-fixation, and labelling of proteins of interest in EM sample are available. The service also includes Elemental analysis via EDX. | EURO-BIOIMAGING | Dutch CLEM Node | complete access | https://www.eurobioimaging.eu/nodes/correlative-light-microscopy-dutch-flagship-node |
2.15w+2.46w | NL | High Field Magnetic Resonance Imaging (human) | Human high field Magnetic resonance imaging (MRI), comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level. MRI scanners at high magnetic fields have the advantage to significantly increase the sensitivity and thus improve the image resolution and scan duration. This service is provided on a 7T scanner. | EURO-BIOIMAGING | Dutch High Field MR Hub | complete access | https://www.eurobioimaging.eu/nodes/dutch-high-field-imaging-hub |
2.15x+2.46x_HF MRI | NL | High Field Magnetic Resonance Imaging (human) | Human high field Magnetic resonance imaging (MRI), comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level. MRI scanners at high magnetic fields have the advantage to significantly increase the sensitivity and thus improve the image resolution and scan duration. This service is provided on a 9.4T scanner. | EURO-BIOIMAGING | Dutch High Field MR Hub | complete access | https://www.eurobioimaging.eu/nodes/dutch-high-field-imaging-hub |
2.15y+2.46y | NL | High Field Magnetic Resonance Imaging (human) | Human high field Magnetic resonance imaging (MRI), comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level. MRI scanners at high magnetic fields have the advantage to significantly increase the sensitivity and thus improve the image resolution and scan duration. This service is provided on a 7T scanner. | EURO-BIOIMAGING | Dutch High Field MR Hub | complete access | https://www.eurobioimaging.eu/nodes/dutch-high-field-imaging-hub |
2.15z+2.46z_MRI | NL | Preclinical Magnetic Resonance Imaging | Magnetic resonance imaging (MRI) of animal models, comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level. Used to monitor disease development and to test the efficacy of therapeutic treatments, to develop novel diagnostic tools etc. The service includes animal model preparation, provision of contrast agents, image data processing and analysis. | EURO-BIOIMAGING | Preclinical Imaging Centre | Hour | https://www.eurobioimaging.eu/nodes/preclinical-imaging-centre-(prime)---molecular-imaging-dutch-node |
2.12s+2.33s+2.40s_NorMIC | NO | Advanced Light Microscopy and standard Electron Microscopy | Advanced Light Microscopy Imaging, including confocal, super-resolution microscopy through SIM or single-molecule localisation, functional imaging for protein dynamics and interactions (FRET, FRAP), TIRF, and live-cell imaging and high-speed imaging. | EURO-BIOIMAGING | Advanced Light Microscopy Node Oslo - NorMIC | hour | https://www.eurobioimaging.eu/nodes/normic-oslo---advanced-light-microscopy-node-oslo |
2.15aa+2.46aa_NORMOLIM | NO | Preclinical Magnetic Resonance Imaging | Magnetic resonance imaging (MRI) of animal models, comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level. Used to monitor disease development and to test the efficacy of therapeutic treatments, to develop novel diagnostic tools etc. The service includes animal model preparation, provision of contrast agents, image data processing and analysis. | EURO-BIOIMAGING | NORMOLIM - Norwegian Molecular Imaging Infrastructure | hour | https://www.eurobioimaging.eu/nodes/normolim,-norwegian-molecular-imaging-infrastructure |
2.15a+2.46a_Inveon | AT | Preclinical PET-CT and SPECT-CT imaging | Positron Emission Tomography (PET) and Single Photon Emission Tomography (SPECT) Imaging of animal models, for monitoring function and metabolism. PET and SPECT imaging can be performed as single modality acquisition but can also be used in combination with CT, for precise spatial localization of the PET/SPECT signals within the subject. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. | EURO-BIOIMAGING | Austrian BioImaging | hour | https://www.eurobioimaging.eu/nodes/austrian-bioimagingcmi |
2.15ab+2.46ab_NORMOLIM | NO | Preclinical PET imaging | Positron Emission Tomography (PET) Imaging of animal models, for monitoring function and metabolism. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. | EURO-BIOIMAGING | NORMOLIM - Norwegian Molecular Imaging Infrastructure | hour | https://www.eurobioimaging.eu/nodes/normolim,-norwegian-molecular-imaging-infrastructure |
2.15ac+2.46ac_NORMOLIM | NO | Preclinical UltraSound imaging | UltraSound Imaging of animal models for visualizing and quantifying tumors, allowing staging, monitoring of therapeutic treatments, or guided injection of drugs. The use of targeting microbubbles as US contrast agents allows the visualization and quantification of specific tumor biomarkers as well. The service includes animal model preparation, probes, image data processing and analysis. | EURO-BIOIMAGING | NORMOLIM - Norwegian Molecular Imaging Infrastructure | hour | https://www.eurobioimaging.eu/nodes/normolim,-norwegian-molecular-imaging-infrastructure |
2.12t+2.33t+2.40t_ALM | PL | Advanced Light Microscopy | Advanced Light Microscopy Imaging, including confocal, super-resolution microscopy through SIM, STED or single-molecule localisation, functional imaging for protein dynamics and interactions (FRET, FRAP, FLIM, FCCS), TIRF, microdissection and two-photon microscopy. | EURO-BIOIMAGING | Advanced Light Microscopy Node Poland | hour | https://www.eurobioimaging.eu/nodes/advanced-light-microscopy-node-poland |
2.14r_ALM | PL | Electron Microscopy | A range of Electron Microscopy techniques are available, including SEM, TEM, volumeEM in the form of serial section TEM, and Eletron tomography. This also includes methods for labelling proteins of interest in EM samples | EURO-BIOIMAGING | Advanced Light Microscopy Node Poland | Hour | https://www.eurobioimaging.eu/nodes/advanced-light-microscopy-node-poland |
2.15ad+2.46ad_MRI | PT | Preclinical Magnetic Resonance Imaging | Magnetic resonance imaging (MRI) of animal models, comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level. Used to monitor disease development and to test the efficacy of therapeutic treatments, to develop novel diagnostic tools etc. The service includes animal model preparation, provision of contrast agents, image data processing and analysis. | EURO-BIOIMAGING | Brain Imaging Network | hour | https://www.eurobioimaging.eu/nodes/brain-imaging-network-bin |
2.12u+2.33u+2.40u_Instituto | PT | Advanced Light Microscopy and Electron Microscopy | Different fluorescence microscopy methods are offered including expertise in project development and image analysis. The offered methods include super-resolution microscopy techniques such as single molecule localisation, STED, SIM, live cell imaging, mesoscopic imaging via lightsheet and optical projection tomography, functional imaging (FRET, FRAP, FLIM, FCCS), intravital microscopy, high-throughput imaging and correlative techniques. This also includes support techniques such as expansion microscopy, tissue clearing and microdissection | EURO-BIOIMAGING | Portuguese Platform of BioImaging PPBI | hour | https://www.eurobioimaging.eu/nodes/portuguese-platform-of-bioimaging-ppbi |
2.14s | SE | Electron Microscopy | Electron Microscopy techniques, including SEM, TEM, volumeEM, and Eletron tomography. Volume EM methods available include array tomography, serial section TEM, and FIB-SEM. This also includes support for correlative methods and different sample preparations, such as chemical and cryo-fixation. Different methods for labelling proteins of interest in EM are available. Various cryo-EM methods can also be requested | EURO-BIOIMAGING | Swedish National Microscopy Infrastructure | hour | https://www.eurobioimaging.eu/nodes/swedish-national-microscopy-infrastructure-nmi |
2.12v+2.33v+2.40v+2.13j+2.41j_NMI | SE | Advanced Light Microscopy | Advanced Light Microscopy methods, including super-resolution (SMLM, STED, SIM) and functional imaging (FRET, FLIM, FRAP, FCCS), intravital microscopy, mesoscopic imaging with lightsheet, lattice lightsheet, feedback microscopy, expansion microscopy and other specialised and support methods. | EURO-BIOIMAGING | Swedish National Microscopy Infrastructure | hour | https://www.eurobioimaging.eu/nodes/swedish-national-microscopy-infrastructure-nmi |
2.25a_LIP | FR | Molecular profiling of a patient-derived xenograft (PDX) model. | Generation of molecular profiles (e.g., DNA-seq, RNA-seq, Immunohistochemistry, others) on a PDX model, either provided by the user or selected from the EurOPDX collection. The standard service (1x Unit of Access) involves generating one type of profile on one sample. To perform more complex studies, more units of access must be selected. For instance, for a combined DNA-seq/RNA-seq profiling two Units of access are required. The service can be combined with drug efficacy testing, e.g., to profile PDXs after in vivo treatment. | UNITO | Institut Curie | 1 PDX model | https://dataportal.europdx.eu/ |
2.25b_MCCA | NL | Molecular profiling of a patient-derived xenograft (PDX) model. | Generation of molecular profiles (e.g., DNA-seq, RNA-seq, Immunohistochemistry, others) on a PDX model, either provided by the user or selected from the EurOPDX collection. The standard service (1x Unit of Access) involves generating one type of profile on one sample. To perform more complex studies, more units of access must be selected. For instance, for a combined DNA-seq/RNA-seq profiling two Units of access are required. The service can be combined with drug efficacy testing, e.g., to profile PDXs after in vivo treatment. | UNITO | Netherlands Cancer Institute | 1 PDX model | https://dataportal.europdx.eu/ |
2.30a_LIP | FR | In vivo drug efficacy testing on a selected patient-derived xenograft (PDX) model. | Performance of an in vivo drug efficacy study on a selected patient-derived xenografts (PDX) model. The standard service (1x Unit of Access) consists in testing a user-provided drug or compound in a 2-arms study, one control arm and one treatment arm, with 6 NSG mice/arm, and includes a prior tolerability study (3 NSG mice/arm). To perform more complex studies, more units of access must be selected. For instance, for a 4 arms study (e.g., vehicle arm; drug A arm; drug B arm; drug A + drug B arm), two units of access are required. The user could select the PDX model on the EurOPDX Data Portal (https://dataportal.europdx.eu/), supported by the Research Infrastructure. The costs for shipment or, in case, for buying commercial drug(s) or compound(s) to be used in the study are not included in the service. | UNITO | Institut Curie | 1 PDX model | https://dataportal.europdx.eu/ |
2.30b_MCCA | NL | In vivo drug efficacy testing on a selected patient-derived xenograft (PDX) model. | Performance of an in vivo drug efficacy study on a selected patient-derived xenografts (PDX) model. The standard service (1x Unit of Access) consists in testing a user-provided drug or compound in a 2-arms study, one control arm and one treatment arm, with 6 NSG mice/arm, and includes a prior tolerability study (3 NSG mice/arm). To perform more complex studies, more units of access must be selected. For instance, for a 4 arms study (e.g., vehicle arm; drug A arm; drug B arm; drug A + drug B arm), two units of access are required. The user could select the PDX model on the EurOPDX Data Portal (https://dataportal.europdx.eu/), supported by the Research Infrastructure. The costs for shipment or, in case, for buying commercial drug(s) or compound(s) to be used in the study are not included in the service. | UNITO | Netherlands Cancer Institute | 1 PDX model | https://dataportal.europdx.eu/ |
2.31b_MEDINA | ES | Advanced profiling services including in-vitro efficacy, toxicological, ADME, stability, PK and PD investigations of confirmed hit compounds | ADME-TOX studies platform include drug metabolism studies (CYP inhibition (6 isoforms)and CYP induction, drug metabolites identification, metabolic stability (hepatocytes and liver microsomes), plasma protein binding, plasma stability, reactive metabolite assessment),bioanalysis and PK analysis, drug absorption PAMPA, physicochemical profiling ( thermodynamic solubility, chemical and kinetic stability), toxicity ( cytotoxicity, cardiotoxicity, genotoxicity AMES and Micronucleus test, neurotoxicity) | EU-OPENSCREEN | Fundación Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía | ADME-tox panel of assays (for 10 compounds) | https://www.medinadiscovery.com/ https://www.es-openscreen.com/partner/fundacion-medina/ |
2.31c_SINTEF | NO | Advanced profiling services including in-vitro efficacy, toxicological, ADME, stability, PK and PD | Advanced profiling services including in-vitro efficacy, toxicological, ADME, stability, PK and PD investigations of confirmed hit compounds | EU-OPENSCREEN | SINTEF | in-vitro immunotox. | https://www.sintef.no |
2.32a_USC | ES | High-content image-based profiling of compounds and high-throughput microscopy services, including those applied on patient-derived cells | The INNOpharma Screening platform has state-of-the-art equipment for screening campaigns with different technological approaches. | EU-OPENSCREEN | University of Santiago de Compostela | 1-month project ( HCS compound prof) | https://www.usc.gal/en/department/pharmacology-pharmacy-and-pharmaceutical-technology |
2.32b_ITMP | DE | High-content image-based profiling of compounds and high-throughput microscopy services, including those applied on patient-derived cells | Establisihing assays and screening for viability, proliferation and multi-parametric high-content microscopy readouts and profiling batches of up to 50 compounds. | EU-OPENSCREEN | Fraunhofer Institute for Translational Medicine and Pharmacology | compound profiling | https://www.itmp.fraunhofer.de/ |
2.32c_MEDINA | ES | High-content image-based profiling of compounds and high-throughput microscopy services, including those applied on patient-derived cells | Fully integrated fluorescence imaging platform and associated software for bio-imaging assays with primary and established cell lines; multiparametric high content imaging with broad range of target-based and phenotype-based assays (kinetic or single endpoint live-cells analysis; broad fluorophore choice for multiplexing); customized project from experimental approach to data analysis. Determination cell toxicity effects, cell functions, cell signaling pathways , cell structures and morphological features | EU-OPENSCREEN | Fundación Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía | compound profiling (for 10 compounds) | https://www.medinadiscovery.com/ https://www.es-openscreen.com/partner/fundacion-medina/ |
2.35_MEDINA | ES | Validation of the binding affinity and kinetics of compounds towards the drug target via biophysical methods, e.g., SPR, ITC, DSF | Application of biophysical methods to determine the binding affinity and binding kinetics of compounds to the selected target | EU-OPENSCREEN | Fundación Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía | DSF analysis (for 10 compounds) | https://www.medinadiscovery.com/ https://www.es-openscreen.com/partner/fundacion-medina/ |
2.31a_CIPF | ES | Advanced profiling services including in-vitro efficacy, toxicological, ADME, stability, PK and PD investigations of confirmed hit compounds | Validation assays to support hit identification ranging from cell to molecular biology and adapting to the applicat request. Alternatively, in-vivo assays in wild type mice for 1 candidate + control group could be performed to determine PK studies by LC-MS or toxicological studies by biochemical markers and histology of major organs. Intravenous adminisration, subcutaneous, intraasal, intraperitoneal or oral administration could be performed. | EU-OPENSCREEN | Centro de Investigación Príncipe Felipe | validation assay | http://www.VicentResearchLab.com |
2.29b_MEDINA | ES | Consultation and support in the development of disease-relevant biochemical, pathway-based and cell-based 2D or 3D assays for compound screening or hit validation | Expert assessment and technical support in the development and miniaturization of new biochemical and phenotypic assays to be run in high throughput screening platforms for hit discovery and hit validation follow-up | EU-OPENSCREEN | Fundación Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía | Hour of consult | https://www.medinadiscovery.com/ https://www.es-openscreen.com/partner/fundacion-medina/ |
2.29a_CIPF | ES | Consultation and support in the development of disease-relevant biochemical, pathway-based and cell-based 2D or 3D assays for compound screening or hit validation | Advise to be given by phone or by email or by TC/VC, in any doubt concerning the stablishment and use of 2D and 3D based phenotypic assays. Homospheroids, heterospheroids or organoids from commercially available cell lines or patient derived. As well as any adequate confirmation assays for hit validation. | EU-OPENSCREEN | Centro de Investigación Príncipe Felipe | hour of consultation | http://www.VicentResearchLab.com |
2.6_I2PC_EM | ES | Electron Microscopy Image Analysis, I2PC, Madrid, Spain - canSERV | This platform will provide personalised support to projects requiring specialised processing of either electron or X-ray microscopy images. It will also help developers in integrating their methods into generally accessible analysis workflows. The Instruct Image Processing Centre (I2PC) in Madrid will assign personnel to this analysis, as well as the required computational resources. | INSTRUCT-ERIC | Instruct Centre ES - Instruct Image Processing Centre | 1day | |
2.5+2.22+2.47_Instruct | CZ | Electron Microscopy, Brno, Czech Republic - canSERV | Access to state-of-the-art instrumentation for electron cryo-microscopy and user support in sample preparation, data acquition, and data processing. Services include high-throughput single particle analysis (SPA) data collection, cryo-electron tomography data acquisition, cryo-FIB lamella preparation, electron diffraction tomography (micro-ED) data acquisition, data analysis, sample preparation and cryo-EM screening, resin embedding, FIB-SEM tomography. | INSTRUCT-ERIC | Instruct Centre CZ - CEITEC | 1 day | https://www.ceitec.eu |
2.10+2.19_instruct | FI | X-ray Diffraction, Oulu, Finland - canSERV | Biocenter Oulu Protein Crystallography core facility offers crystallization using nanoliter dispensers and a collection of commercial and self maintained crystallization screens. Crystallization is followed using the IceBear software and remote access to monitor the experiments can be provided following the visit. Biocenter Oulu Protein Crystallography core facility further offers home-source X-ray generator for macromolecular crystal diffraction experiments. | INSTRUCT-ERIC | Instruct Centre FI - University of Oulu | 1 day | |
2.4+2.39_Instruct | CZ | Protein Production, Vestec near Prague, Czech Republic - canSERV | The core facility for protein production provides services covering DNA cloning and DNA plasmid preparation, protein expression in E. coli expression systems and recombinant protein purification. Our services can also include optimization of expression and purification protocols, protein identity and purity testing and determination of protein concentration. | INSTRUCT-ERIC | Instruct Centre CZ - BIOCEV | 1 day | https://www.biocev.eu |
2.45_Instruct | NL | PDB-REDO, Amsterdam, Netherlands - canSERV | PDB-REDO is a procedure to optimise crystallographic structure models, providing algorithms that make a fully automated decision making system for refinement, rebuilding and validation. With PDB-REDO you can obtain updated and optimised versions of existing entries of the PDB from our DataBank, or you can optimise your own structure model using our Server. Due to robust automation, the procedure can be run at scales from individual structures to high-throughput optimization of thousands of structures. If you want to know more, please check https://pdb-redo.eu | INSTRUCT-ERIC | Instruct Centre NL - Netherlands Cancer Institute | 1 day | https://www.nki.nl/research/facilities-platforms/protein-facility/ |
2.7+2.19+2.23+2.37_Instruct | CZ | Solution NMR, Brno, Czech Republic - canSERV | The Core Facility of High Field NMR Spectroscopy at CEITEC Instruct centre provides access to NMR spectrometers in the range of proton frequencies from 500 MHz to 950 MHz. The equipment is suited mainly to the studies of structure, dynamics and interactions of biomolecules, i.e. proteins, nucleic acids and carbohydrates. However, the instrumentation is flexible enough to cover various research needs in material science, organic and inorganic chemistry, biochemistry, biology and biophysics. | INSTRUCT-ERIC | Instruct Centre CZ - CEITEC | 1 day | https://www.ceitec.eu |
2.3+2.21+2.38_Instruct | BE | Nanobody Services, Brussels, Belgium - canSERV | This service includes the generation and characterisation of conformational nanobodies (to facilitate the structural analysis of proteins that are notoriously difficult to purify, to crystallize or to study by another method), as well as rapid selection of nanobodies by screening/affinity maturation. Nanobodies can also be functionally expressed as intrabodies in eukaryotic cells, therefore these single-domain antibodies can also be used to track their targets inside a living cell. | INSTRUCT-ERIC | Instruct Centre BE - Nanobodies4Instruct | 1 day | https://www.steyaertlab.be |
2.8+2.20+2.23+2.35_Instruct | CZ | Native Mass Spectrometry and Molecular Biophysics, Vestec near Prague, Czech Republic - canSERV | Services include various methodologies for the characterisation of protein structures, identification and quantification of proteins, precise determination of protein molecular mass, characterisation of post translational modifications, analysis of kinetic and thermodynamic parameters of biomolecular interactions, and biophysical characterisation of the structure, function and stability of biological macromolecules like proteins, nucleic acids, lipids and their complexes. The service provided includes data processing and reporting ready for publication. | INSTRUCT-ERIC | Instruct Centre CZ - BIOCEV | 1 day | https://www.biocev.eu |
2.9+2.36_Instruct | FR | CrystalDirect Automated Fragment Screening Pipeline, EMBL, Grenoble - canSERV | EMBL Grenoble offers access to a fully automated protein to structure pipeline based on the CrystalDirect technology and the Crystallization Information Management System (CRIMS) software. The CrystalDirect technology enables automated crystal soaking making it possible to support automated, large scale small molecule and Fragment Screening through crystallography efficiently and in a short time. | INSTRUCT-ERIC | Instruct Centre EMBL - EMBL Grenoble | 1 day | www.embl.fr/services/index.html |
*NEW 2.15f + 2.46f_VEVO US_a | CZ | Preclinical Photocoustic imaging | Photoacoustic Imaging (PAI) of animal models, combining the advantages of optical and US imaging technologies. The US signal makes it possible to achieve an increase of both spatial resolution (micrometric) and penetration depth with respect to optical imaging and reduces scattering phenomena. In comparison with US a wider range of molecules can be used as PAI contrast agents. PAI allows to get 3D-high resolution images of soft tissues which provide anatomical, functional, and molecular information. The service includes animal model preparation, probes and image data processing and analysis. | EURO-BIOIMAGING | CAPI | hour | https://www.eurobioimaging.eu/nodes/center-for-advanced-preclinical-imaging-capi |
*NEW 2.17b + 2.15i + 2.46i_SPECT/CT_a | FI | Intravital microscopy | A range of microscopy modalities used for imaging of living animal models, for the real-time visualization of cellular processes in their native environment in the living organism. It can be performed as repetitive imaging on the same animal, allowing biological processes to be tracked over long periods of time, or as acute experiments. Intravital microscopy can be used e.g. for the monitoring of the migration and cell-cell or cell-matrix interactions of invasive/metastatic tumor cells in different tissues. | EURO-BIOIMAGING | FiBI | complete access | https://www.eurobioimaging.eu/nodes/finnish-biomedical-imaging-node |
*NEW 2.17c_SCANOMED_a | HU | Preclinical PET-MRI imaging | Positron Emission Tomography (PET) and Single Photon Emission Tomography (SPECT) Imaging of animal models, for monitoring function and metabolism. PET and SPECT imaging can be performed as single modality acquisition but can also be used in combination with CT, for precise spatial localization of the PET/SPECT signals within the subject. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. | EURO-BIOIMAGING | Medical&Preclinical HU | complete access | https://www.eurobioimaging.eu/nodes/medical-and-preclinical-imaging-hungary |
*NEW 2.15k + 2.46k + 2.17d_Semmelweis_a | HU | Preclinical PET-MRI imaging | Positron Emission Tomography (PET) and Single Photon Emission Tomography (SPECT) Imaging of animal models, for monitoring function and metabolism. PET and SPECT imaging can be performed as single modality acquisition but can also be used in combination with CT, for precise spatial localization of the PET/SPECT signals within the subject. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. | EURO-BIOIMAGING | Medical&Preclinical HU | complete access | https://www.eurobioimaging.eu/nodes/medical-and-preclinical-imaging-hungary |
*NEW 2.15k + 2.46k + 2.17d_Semmelweis_b | HU | Preclinical Magnetic Resonance Imaging | Magnetic resonance imaging (MRI) of animal models, comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level. Used to monitor disease development and to test the efficacy of therapeutic treatments, to develop novel diagnostic tools etc. Several quantitative MR imaging biomarkers can be used to monitor disease progression over time. The service includes animal model preparation, provision of contrast agents, image data processing and analysis. | EURO-BIOIMAGING | Medical&Preclinical HU | complete access | https://www.eurobioimaging.eu/nodes/medical-and-preclinical-imaging-hungary |
*NEW 2.15k + 2.46k + 2.17d_Semmelweis_c | HU | Preclinical UltraSound Imaging | UltraSound Imaging of animal models for visualizing and quantifying tumors, allowing staging, monitoring of therapeutic treatments, or guided injection of drugs. The use of targeting microbubbles as US contrast agents allows the visualization and quantification of specific tumor biomarkers as well. The service includes animal model preparation, probes, image data processing and analysis. | EURO-BIOIMAGING | Medical&Preclinical HU | complete access | https://www.eurobioimaging.eu/nodes/medical-and-preclinical-imaging-hungary |
*NEW 2.15k + 2.46k + 2.17d_Semmelweis_d | HU | Preclinical X-Rays Computed Tomography | X-rays Computed Tomography (CT) on tissues, organs or living animal models. CT contrast agents (usually based on Iodine, Barium, Gold) can be used to enhance the contrast. Preclinical microCT systems can be stand alone or integrated with other modalities like optical or nuclear medicine imaging in order to perform multimodal imaging. The service includes animal model prepartion, contrast agents if needed, image data processing and analysis. | EURO-BIOIMAGING | Medical&Preclinical HU | complete access | https://www.eurobioimaging.eu/nodes/medical-and-preclinical-imaging-hungary |
*NEW 2.15q + 2.46q_micro PET_a | IT | Preclinical Magnetic Resonance Imaging | Magnetic resonance imaging (MRI) of animal models, comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level. Used to monitor disease development and to test the efficacy of therapeutic treatments, to develop novel diagnostic tools etc. Several quantitative MR imaging biomarkers can be used to monitor disease progression over time. The service includes animal model preparation, provision of contrast agents, image data processing and analysis. | EURO-BIOIMAGING | Neuromed | complete access | https://www.eurobioimaging.eu/nodes/digital-imaging-multimodal-platform-neuromed---dimp-neuromed |
*NEW 2.15ad + 2.46ad_MRI_a | PT | Human Magnetic Resonance Imaging | Human Magnetic resonance imaging (MRI). The service comprises support for subjects' enrolment and management of ethical requirements. At BIN, MRI can be used either as a single modality or paired to PET. | EURO-BIOIMAGING | Brain Imaging Network | hour | https://www.eurobioimaging.eu/nodes/brain-imaging-network-bin |
*NEW2.12k + 2.33k + 2.40k_Semmelweis_1 | HU | Atomic Force Microscopy | Atomic Force Microscopy (AFM) is a technique allowing the characterization of sample morphology at nanoscale. Images are acquired by raster scanning a nanometric tip in gentle contact or intermittent contact with the sample. The tip is positioned at the end of a micrometric force transducer, the AFM cantilever: it records the variation of sample topography due to changes of tip-sample interaction during scanning operation. In addition, by measuring the tip-sample interaction force as a function of the tip-sample distance, AFMs can evaluate sample elastic and viscous properties. | EURO-BIOIMAGING | Debrecen University | complet access | https://www.eurobioimaging.eu/nodes/cellular-imaging-hungary |
*NEW2.12s + 2.33s + 2.40s_NorMIC_2 | NO | Electron Microscopy | In transmission electron microscopy (TEM) the accelerated beam of electrons is transmitted through the sample and collected to form an image. To achieve this the specimen is usually sectioned into ultrathin sections less than 100 nm thick. Different methods for sample preparation are available | EURO-BIOIMAGING | University of Oslo | hour | https://www.eurobioimaging.eu/nodes/normic-oslo---advanced-light-microscopy-node-oslo |
*NEW 2.12u + 2.33u + 2.40u_Instituto_2 | PT | Electron Microscopy | Transmission electron microscopy for high-resolution sample imaging, including immuno-labelling and in-resin labelling | EURO-BIOIMAGING | Instituto de Biologica Molecular e Celular - IBCM | hour | https://www.eurobioimaging.eu/nodes/portuguese-platform-of-bioimaging-ppbi |
*NEW 2.5 + 2.22 + 2.47_Instruct_EMBL_HD | DE | Electron Microscopy and Cryo-CLEM, Heidelberg, EMBL - canSERV | The Imaging Centre at EMBL Heidelberg gives access to state-of-the-art cryo-EM equipment for structure determination projects using the latest technology and methods in single-particle analysis, cryo-electron tomography (cryo-ET) and Cryo-correlative light and electron microscopy (Cryo-CLEM). | INSTRUCT-ERIC | Instruct Centre EMBL - EMBL Heidelberg | 1 day | www.embl-hamburg.de/services/index.html |
*NEW 2.5 + 2.22 + 2.47_Instruct_UH | FI | Electron Microscopy, Helsinki, Finland - canSERV | High-resolution cryoEM imaging of protein complexes to study their structure, assembly and function. Access is especially suitable for those wanting to screen samples and get small prelimianary datasets that can be used as proof of principle for access to 300 kV machines or for longer data collection. Correlative light microscopy and cryoEM is possible. Work with genetically modified organisms and BSL2 samples possible (please contact facility in advance). We accept both visits and shipped samples. Biomolecular complexes studied by cryoEM can be purified in the Biocomplex purification facility located in the same building. | INSTRUCT-ERIC | Instruct Centre FI - University of Helsinki | 1 day | www.helsinki.fi/en |
*NEW 2.5 + 2.22 + 2.47_Instruct_FR1 | FR | Electron Microscopy, Strasbourg, France - canSERV | The center offers access for three-dimensional structure determination using cryo-electron microscopy (cryo-EM). The centre has expertise in all aspects of single particle analysis from the automated acquisition of large data sets to image analysis. | INSTRUCT-ERIC | Instruct Centre FR1 - IGBMC | 1 day | www.igbmc.fr |
*NEW 2.5 + 2.22 + 2.47_Instruct_FR2 | FR | Electron Microscopy, Grenoble, France - canSERV | Services include quality control negative staining experiments prior to setting up cryo conditions; Data acquisition (single particle imaging and single axis tomography); Cellular EM services, such as plastic embbeding, tokuyasu method, high pressure freezing, immuno-labelling, ultra thin sectionning at room temperature and cryo. The slices can then be observed using a T12 or F20 electron microscope. | INSTRUCT-ERIC | Instruct Centre FR2 - IBS-ISBG | 1 day | www.ibs.fr/?lang=en |
*NEW2.5 + 2.22 + 2.47_Instruct_NECEN | NL | Electron Microscopy, NeCEN, Leiden, The Netherlands - canSERV | NeCEN is an open access facility for high resolution cryo-electron microscopy of biological samples. Two state-of-the-art Titan Krios transmission electron microscopes allow efficient and automated high resolution data collection in its two most broadly used forms, single particle analysis (SPA) and cryo-electron tomography (cryo-ET). These imaging techniques allow visualisation of biomolecular structures, such as proteins, macromolecular complexes, bacteria, and cell organelles at subnanometer resolution in close-to-native conditions. The facility is equipped with a BSL2 lab and microscope rooms. | INSTRUCT-ERIC | Instruct Centre NL - NeCEN LU | 1 day | www.necen.nl |
*NEW 2.5 + 2.22 + 2.47_Instruct_Diamond | UK | Electron Microscopy, Diamond Light Source, Harwell, UK - canSERV | The Electron Bio-Imaging Centre (eBIC) provides scientists with state-of-the-art experimental equipment and expertise in the field of cryo-electron microscopy, for both single particle analysis and cryo-tomography. Currently eBIC houses five Titan Krios microscopes, a Talos Arctica, Glacios, and a Scios and Aquilos cryo-FIB/SEM. | INSTRUCT-ERIC | Instruct Centre UK - Diamond Light Source | 1 day | www.diamond.ac.uk |
*NEW2.5 + 2.22 + 2.47_Instruct_ABSL | UK | Electron Microscopy, Leeds, UK - canSERV | Transmission electron microscopy of biological specimens, from small macromolecular complexes to cells, tissues and organisms. We have two state-of-the-art Titan Krios microscopes equipped with direct electron detectors (DED) for high resolution imaging. Both instruments have AFIS and Fringe free imaging. | INSTRUCT-ERIC | Instruct Centre UK - Astbury Biostructure Laboratory | 1 day | www.astbury.leeds.ac.uk |
*NEW2.5+2.22 + 2.47_Instruct_OPIC | UK | Electron Microscopy, Oxford, UK - canSERV | Access to state-of-the-art equipment for cryo-EM high-resolution single particle analysis, in-situ FIB-SEM and cryo-ET of biological specimens, from small macromolecular complexes to cells. Our facility includes equipment for grid preparation and optimisation (plunging devices and grid micro-patterning) and data collection (Titan Krios G3i, Aquilos 2). Equipment is located within biosafety containment laboratories (ACDP category 3 and DEFRA 4). | INSTRUCT-ERIC | Instruct Centre UK - Oxford Particle Imaging Centre | 1 day | www.opic.ox.ac.uk |
*NEW 2.9 + 2.36_Instruct_Diamond | UK | XChem Fragment Screening, Diamond Light Source, Harwell, UK - canSERV | Fragment screening through Macromolecular Crystallography at Diamond’s XChem facility at beamline I04-1, supporting 1000+ compound screens | INSTRUCT-ERIC | Instruct Centre UK - Diamond Light Source | 1 day | www.diamond.ac.uk |
*NEW2.8 + 2.20 + 2.23 + 2.35_Instruct_ULiege | BE | Robotein Automated Biomolecular Analysis, Liege, Belgium - canSERV | Numerous high-throughput (HT) assays are offered, such as HT biophysical characterisation (conformational stability (△G0) of proteins in the presence of chaotropic agents (e.g. urea, guanidinium chloride)); HT binding affinity measurements (enables real-time and label-free determination of binding affinity); HT protein quantitation form crude extracts (fast and accurate protein quantitation from crude extracts (cell extracts, culture media, etc.), with no required purification steps; particularly suitable to measure the concentration of full-length antibodies or various antibody fragments); HT enzymatic assays; HT screen of pH/buffer components (Protein formulation); HT screen of protein refolding conditions from inclusion bodies; Plasmaprofiling for metabolomics | INSTRUCT-ERIC | Instruct Centre BE - Robotein for Instruct | 1 day | http://www.robotein.ulg.ac.be/ |
*NEW 2. 2.8 + 2.20 + 2.23 + 2.35_3.2_Instruct_CEITEC | CZ | Proteomic Mass Spectrometry and Molecular Biophysics, Brno, Czech Republic - canSERV | Complete mass spectrometry-assisted proteomics services from sample preparation/purification to qualitative/quantitative characterisation of proteins and their modifications by mass spectrometry and data processing. Services also comprise biophysical characterization of (bio)molecules and the study of biomolecular interactions (determination of affinity, kinetic and thermodynamic parameters). Techniques include analytical ultracentrifugation (AUC), isothermal titration calorimetry (ITC), surface plasmon resonance (SPR), bio-layer interferometry (BLI), microscale thermophoresis (MST), dynamic light scattering (DLS), differential scanning calorimetry (DSC), differential scanning fluorimetry (DSF), and analytical size-exclusion chromatography (SEC-MALS). | INSTRUCT-ERIC | Instruct Centre CZ - CEITEC | 1 day | www.ceitec.eu |
*NEW2. 2.8 + 2.20 + 2.23 + 2.35_3.2_Instruct_UEF | FI | Native Mass Spectrometry, Joensuu, Finland - canSERV | UEF core facility offers two state-of-the-art mass spectrometers for versatile utilization of native mass spectrometry for studies of protein folding and assembly of biological macromolecules as well as for quantitative biological interaction studies with large dynamic range. | INSTRUCT-ERIC | Instruct Centre FI - University of Eastern Finland | 1 day | https://instruct-eric.org/centre/university-of-eastern-finland |
*NEW. 2.8 + 2.20 + 2.23 + 2.35_3.2_Instruct_UH | FI | Single-cell Proteomics (SCoPE-MS), Helsinki, Finland - canSERV | SCoPE-MS offers a revolutionary technique enabling global level identification and quantification of single cell proteome (>1000 proteins) using mass spectrometry, exceeding the by >ten-fold the identification capability of fluorescence or mass cytometry. Single cell technologies on DNA and RNA level have recently become extremely popular, the SCoPE-MS will extend this to protein/proteome level. | INSTRUCT-ERIC | Instruct Centre FI - University of Helsinki | 1 day | www.helsinki.fi/en |
2. 2.8 + 2.20 + 2.23 + 2.35_3.2_Instruct_UU | NL | Mass Spectrometry, Utrecht, Netherlands - canSERV | Mass spectrometry based proteomics enables the identification and quantification of cellular proteins and their modified forms. The Biomolecular Mass Spectrometry and Proteomics group at the Bijvoet Centre provides access to qualitative proteomics analysis of proteins in complex samples. Instrumentation available includes advanced (2D)-HPLC and state-of-the-art mass spectrometers such as Thermo Scientific Q-Exactive Orbitraps. | INSTRUCT-ERIC | Instruct Centre NL - Bijvoet Centre | 1 day | https://www.uu.nl/en/research/bijvoet-centre-for-biomolecular-research |
2.8 + 2.20 + 2.23 + 2.35_Instruct_ICSAS | SK | Mass Spectrometry - Glycan Analysis, Bratislava, Slovakia - canSERV | Glycan structure analysis by applying spectroscopic methods, such as Protein Mass spectrometry using an Orbitrap Elite mass spectrometer, coupled with HPLC/nanoLC or Native Mass Spectrometry using an UltrafleXtreme II MALDI mass spectrometer, coupled with nanoLC | INSTRUCT-ERIC | Instruct Research Site SK - ICSAS | 1 day | www.sav.sk/?lang=en |
2. 2.8 + 2.20 + 2.23 + 2.35_3.2_Instruct_ABSL | UK | Mass Spectrometry, Leeds, UK - canSERV | Services include hydrogen/deuterium exchange (HDX)-MS analyses to determine protein folding states, effects of ligand binding and protein interactions (e.g. epitope mapping); Covalent labelling setup for hydroxyl radical foot printing via Fast Photochemical Oxidation of Proteins (FPOP) linked to mass spectrometry, to probe changes in solvent accessibility; High-resolution native MS (for heterogeneous systems at high mass-to-charge ratios, e.g. membrane protein lipid complexes); Ion mobility capabilities | INSTRUCT-ERIC | Instruct Centre UK - Astbury Biostructure Laboratory | 1 day | www.astbury.leeds.ac.uk |
1. 2.7 + 2.19 + 2.23 + 2.37_3.1_Instruct_GUF | DE | Solution NMR, Frankfurt, Germany - canSERV | This service provides access to NMR spectrometers in the range of proton frequencies from 600 MHz to 950 MHz to elucidate the structure and funtional mechanism of biomolecules, such as proteins, RNA, ligand-protein and ligand-RNA complexes. | INSTRUCT-ERIC | Goethe University Frankfurt | 1 day | |
1. 2.7 + 2.19 + 2.23 + 2.37_3.1_Instruct_UH | FI | NMR, Helsinki, Finland - canSERV | The NMR facility provides services on the field of liquid NMR. The main focus area includes studies of structure and dynamics of biomacromolecules, but also other types of analyses are very well possible e.g. with broadband probe on 600 MHz. | INSTRUCT-ERIC | Instruct Centre FI - University of Helsinki | 1 day | www.helsinki.fi/en |
1. 2.7 + 2.19 + 2.23 + 2.37_3.1_Instruct_FR2 | FR | NMR, Grenoble, France - canSERV | Access to solid state NMR equipment with state-of-the-art magic-angle spinning (MAS) capabilities, and triple-resonance probes for different rotor sizes and spinning speeds. High-sensitivity solution-state NMR applications are also offered using instruments with Bruker electronics (Avance III HD) and cryogenically cooled probes. Standard Bruker experiment libraries and additional in-house libraries for optimised fast NMR data acquisition (e.g. SOFAST, BEST, HADAMAC-type experiments) are available. Fast-mixing equipments required for real-time studies of kinetic processes, e.g. protein folding are also available. | INSTRUCT-ERIC | Instruct Centre FR2 - IBS-ISBG | 1 day | www.ibs.fr/?lang=en |
1. 2.7 + 2.19 + 2.23 + 2.37_3.1_Instruct_CERM | IT | NMR, Florence, Italy - canSERV | Solid-state and solution NMR for structure and dynamic characterisation of biological macromolecules and their complexes; “protonless” NMR experiments and in-cell NMR spectroscopy, tailored pulse sequences for structural determination of paramagnetic systems; Relaxometry (structural and dynamical information on nuclear spin systems); Electron Paramagnetic Resonance (EPR)-based methods to map local dynamic and structural features of biomolecules, to explore different modes of biomolecule-ligand interaction, to obtain long-range structural restraints and to probe metal-ion-binding sites. | INSTRUCT-ERIC | Instruct Centre IT - CERM/CIRMMP | 1 day | www.cerm.unifi.it |
1. 2.7 + 2.19 + 2.23 + 2.37_3.1_Instruct_UU | NL | NMR, Utrecht, Netherlands - canSERV | Solid state and solution NMR equipment for the characterisation of biomolecules, their structure determination and dynamical and functional studies. Services also include structural determination of high molecular weight proteins or complexes, such as nucleosome-protein complexes (200-250 kDa) and other special applications, such as in-cell NMR experiments. Support for applications involving interactions of intrinsically disordered proteins as well as support for medium to high-througput screening. | INSTRUCT-ERIC | Instruct Centre NL - Bijvoet Centre - UU | 1 day | https://www.uu.nl/en/research/bijvoet-centre-for-biomolecular-research |
1. 2.7 + 2.19 + 2.23 + 2.37_3.1_Instruct_ABSL | UK | Solution NMR, Leeds, UK - canSERV | This service offeres solution NMR based on direct heteronuclear detection (15N and 13C, 5mm TXO Cryoprobe). Combination of high field and Carbon detection for complex or large intrinsically disordered proteins and characterisation of protein sidechains. Techniques using 15N detection are under development and are of interest in chemically exchanging systems, as an alternative to Ca detection and when deuteration is not feasible. Solution NMR services for mass limited samples and high ionic strength samples using a smaller diameter probe (3mm TCI Cryoprobe) for optimised sensitivity. | INSTRUCT-ERIC | Instruct Centre UK - Astbury Biostructure Laboratory | 1 day | www.astbury.leeds.ac.uk |
2.4 + 2.39_Instruct_UH | FI | Biomolecular Complex Purification (Biocomplex), Helsinki, Finland - canSERV | Sample preparation and purification for functional and structural studies using preparative ultracentrifugation, monolithic chromatography and asymmetrical flow field-flow fractionation technologies. These can be used in different combinations to purify large macromolecular complexes such as viruses, exosomes, membrane vesicles, large protein complexes, and ribonucleoprotein complexes. Samples produced at Biocomplex can be further studied at the CryoEM facility located in the same building. We accept both visits and shipped samples. | INSTRUCT-ERIC | Instruct Centre FI - University of Helsinki | 1 day | www.helsinki.fi/en |
2.4 + 2.39_Instruct_FR1 | FR | Protein Production and Gene Tagging, Strasbourg, France - canSERV | Recombinant expression of protein and multi-protein complexes in prokaryotic and eukaryotic hosts as well as biomass production for isolation of poorly abundant proteins and endogenous complexes; Gene tagging with the CRISPR-Cas9 system for introduction of affinity tags to facilitate the purification of endogenous complexes or for tagging proteins with fluorescent reporters in view of imaging and functional proteomics applications, in the frame of a partnership with the TacGene platform from the Museum d’Histoire Naturelle (Paris, France). | INSTRUCT-ERIC | Instruct Centre FR1 - IGBMC | 1 day | www.igbmc.fr |
2.4 + 2.39_Instruct_WIS | IL | Protein Production, Weizmann Istitute, Israel - canSERV | Protein production in bacteria, baculovirus, and mammalian cells. Proteins are typically purified following a standard 3 step protocol which includes: affinity capture, ion exchange and size exclusion. Specific techniques can be employed depending on the protein, the experimental requirements, tags and yields. Removal of tags with TEV protease can be employed. | INSTRUCT-ERIC | Instruct Centre IL - Weizmann Institute of Science | 1 day | http://www.weizmann.ac.il/ISPC/ |
2.4 + 2.39_Instruct_NKI | NL | Protein Production in Insect and Mammalian Cells, Amsterdam, Netherlands - canSERV | Protein production using insect and mammalian cells, and assisting users with the design of their expression construct in terms of solubility, domain boundaries, etc. We offer a variety of expression vectors, including a set of LIC vectors (Ligand Independent Cloning). For expression of intracellular and extracellular proteins in insect cells we have SF9 and SF21 systems. Our mammalian expression system has been optimized for HEK-293T cells, and we also have the glycosylation free, gnT1-deficient HEK293S variety. Protein expression in bacteria (E. coli) is beyond the scope of the facility. | INSTRUCT-ERIC | Instruct Centre NL - Netherlands Cancer Institute | 1 day | https://www.nki.nl/research/facilities-platforms/protein-facility/ |
2.4 + 2.39_Instruct_EMBL_HH | DE | Molecular Biophysics, Hamburg, Germany - canSERV | Services for protein quality control in addition to biophysical characterization techniques for studying the interaction of biomolecules, including MALDI (MS), Thermofluor (TF), Circular Dichroism (CD), Fourier-transform infrared spectroscopy (FTIR), Isothermal Titration Calorimetry (ITC), nano Differential Scanning Fluorimetry (nDSF), Surface Plasmon Resonance (SPR), Biolayer interferometry (Octet), Microscale Thermophoresis (MST), Dynamic Light Scattering (DLS), Mass Photometry (Refeyn), Ligand screening kinetics (Creoptix-WAVE) and time resolved reactions (stopped-flow). | INSTRUCT-ERIC | Instruct Centre EMBL - EMBL Hamburg | 1 day | www.embl-hamburg.de/services/index.html |
2.10 + 2.19_Instruct_BIOCEV | CZ | X-ray Diffraction and Bio-SAXS, Vestec near Prague, Czech Republic - canSERV | Crystallization of biomacromolecules and their complexes as well as services for characterization of protein structures using single crystal X-ray diffraction and small-angle X ray scattering for analysis of liquid samples. The quality of high-intense X-ray beams is ensured by using of MetalJet sources (Excillum). The provided services include assistance with data processing and reporting ready for publication. | INSTRUCT-ERIC | Instruct Centre CZ - BIOCEV | 1 day | www.biocev.eu |
2.10 + 2.19_Instruct_EMBL_HH | DE | X-ray Diffraction and Bio-SAXS, Hamburg, Germany - canSERV | Macromolecular crystallography and biological small-angle X-ray scattering (SAXS). SAXS allows one to study the overall (quaternary) structure and structural transitions in a non-restrictive sample environment, directly in solution. Examples of services offered are: (i) combinatorial high throughput ligands/additives screening; (ii) studies of equilibrium mixtures and membrane proteins with online SEC-SAXS coupled with biophysical analysis, and (iii) time-resolved studies of processes, from sub-ms ((un)folding) to hours (oligomerization, fibrillation). Sample preparation and characterisation (crystallization screens suitable for soluble and membrane proteins), processing and evaluation of the data. | INSTRUCT-ERIC | Instruct Centre EMBL - EMBL Hamburg | 1 day | www.embl-hamburg.de/services/index.html |
2.18l_IRST IRCCS_b | IT | RNA sequencing | total RNA or mRNA sequencing in tumoral and/ or non tumoral samples using NGS technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | IRST IRCSS | sample | www.irst.emr.it |
2.18j_CNAG_b | ES | RNA sequencing | total RNA or mRNA sequencing in tumoral and/ or non tumoral samples using NGS technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | CNAG | 1Gb sequence data | https://www.cnag.crg.eu/ |
2.18f_UL Biobank_a | PL | Whole Genome Sequencing | Whole genome sequencing in tumoral and non tumoral samples using next generation sequencing technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | Biobank Lab of the University of Lodz | sample | www.biobank.uni.lodz.pl |
2.18f_UL Biobank_b | PL | RNA sequencing | RNA sequencing in tumoral/ non tumoral samples using next generation sequencing technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | Biobank Lab of the University of Lodz | sample | www.biobank.uni.lodz.pl |
2.18f_UL Biobank_c | PL | Whole Exome Sequencing | Whole exome sequencing in tumoral and non tumoral samples using next generation sequencing technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | Biobank Lab of the University of Lodz | sample | www.biobank.uni.lodz.pl |
2.18f_UL Biobank_d | PL | 16S rRNA microbiome sequencing | Microbial profiling by sequencing 16S ribosomal RNA (rRNA) with next generation sequencing technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | Biobank Lab of the University of Lodz | sample | www.biobank.uni.lodz.pl |
2.18f_UL Biobank_e | PL | Metagenomic microbiome sequencing | Microbial profiling by shotgun metagenomics using next generation sequencing technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | Biobank Lab of the University of Lodz | sample | www.biobank.uni.lodz.pl |
*NEW 2.18k_UNIMIB Med_a | IT | Exome sequencing | Whole exome sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | UNIMIB_MED | cell | https://en.unimib.it/ |
*NEW 2.18k_UNIMIB Med_b | IT | Target DNA sequencing: custom gene panels | Target sequencing of custom gene panels in tumoral and/ or non tumoral samples using next generation sequencing technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | UNIMIB_MED | cell | https://en.unimib.it/ |
*NEW2.18k_UNIMIB Med_c | IT | RNA sequencing | total RNA or mRNA sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | UNIMIB_MED | cell | https://en.unimib.it/ |
2.18l_IRST IRCCS_c | IT | Whole exome sequencing | Whole exome sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | IRST IRCSS | sample | www.irst.emr.it |
2.18l_IRST IRCCS_d | IT | Target DNA sequencing: custom gene panels | Target sequencing of custom gene panels in tumoral and/ or non tumoral samples using next generation sequencing technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | IRST IRCSS | sample | www.irst.emr.it |
2.18j_CNAG_c | ES | Whole exome sequencing | Whole exome sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | CNAG - CRG | 1Gb sequence data | https://www.cnag.crg.eu/ |
2.18j_CNAG_d | ES | Whole genome enzymatic methyl sequencing | Whole genome enzymatic methyl sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | CNAG - CRG | 1Gb sequence data | https://www.cnag.crg.eu/ |
2.18j_CNAG_e | ES | Single cell ATAC sequencing | Single cell ATAC (assay for transposase-accessible chromatin) sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | CNAG - CRG | 1Gb sequence data | https://www.cnag.crg.eu/ |
2.18l_IRST IRCCS_e | IT | smallRNA sequencing | smallRNA sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | IRST IRCSS | sample | www.irst.emr.it |
2.18l_IRST IRCCS_f | IT | Single cell mRNA sequencing | Single cell mRNA sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | IRST IRCSS | sample | www.irst.emr.it |
2.18l_IRST IRCCS_g | IT | Spatial transcriptomics | Spatial transcriptomics in tumoral and/ or non tumoral samples using next generation sequencing technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | IRST IRCSS | sample | www.irst.emr.it |
2.18j_CNAG_f | ES | Single cell mRNA sequencing | Single cell mRNA sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | CNAG - CRG | 1Gb sequence data | https://www.cnag.crg.eu/ |
2.18j_CNAG_g | ES | Single cell immune repertoire profiling | Single cell immune repertoire profiling (TCR/ BCR) in tumoral and/ or non tumoral samples using next generation sequencing technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | CNAG - CRG | 1Gb sequence data | https://www.cnag.crg.eu/ |
2.18j_CNAG_h | ES | Spatial transcriptomics | Spatial transcriptomics in tumoral and/ or non tumoral samples using next generation sequencing technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | CNAG - CRG | 1Gb sequence data | https://www.cnag.crg.eu/ |
2.18u_Single cell _LIED_b | DE | Single cell immune repertoire profiling | Single cell immune repertoire profiling (TCR/ BCR) in tumoral and/ or non tumoral samples using next generation sequencing technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | LIED, University of Lubeck | sample | |
2.18u_Single cell _LIED_c | DE | Spatial transcriptomics | Spatial transcriptomics in tumoral and/ or non tumoral samples using next generation sequencing technologies | BIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) | LIED, University of Lubeck | sample |
More information about individual services, please contact us.