Field 2 – Advanced technologies for Personalised Oncology

This field will provide the cancer research community with access to cutting-edge services and technologies, including biological and biomedical imaging, structural biology methods, genomics and analytical pipelines, as well as the associated expertise in their use, to address cancer research questions at a molecular, cellular and whole organism level. The technologies and services offered are closely interlinked with the services and tools in field 1, 3 and 4. This close interlink and collaboration will provide researchers with the opportunity to build service pipelines combining access to advanced technologies for using and analysing cancer models provided by field 1, as well as the tools and technologies needed in their search and validation of new biomarkers (field 3) and new treatments (field 4).

Unique IDCountryService Name Description of ServiceResearch InfrastructureService ProviderUnit of accesWebsite
2.29d_BRFAAGRTissue slice cultureFresh solid tumors from humans or mice are embedded in agarose and sectioned at 150-300 um using a Compresstome® tissue slicer. Slices can be grown in appropriate media for up to one week in Transwell inserts. They can be used for drug testing, immune cell co-cultures, migration studies and other applications.BBMRI-ERIC Biomedical Research Foundation of the Academy of Athenssamplehttps://www.bioacademy.gr
2.23_DNS_Uo LubeckDEImage CytometryGenomic instability assessment by nuclear DNA ploidy measurements by means of image cytometry using Feulgen stained cytospins or imprints. At least 500 nuclei per sample are selected interactively and the DNA content is measured quantitatively using the ACAS imaging system (Ahrens ACAS, Germany). The DNA profiles are classified according to Auer.BBMRI-ERIC Section for Translational Surgical Oncology & Biobanking, Department of SurgerySamplehttps://www.uni-luebeck.de/ncch/lion.html
2.19e_UZL-qMSDEQuantatitve mass spectrometrySemi-quantitative data-independent mass spectrometry (DIA) making use of a state-of-the-art hybrid trapped ion mobility spectrometry - quadrupole time of flight (TOF) mass spectrometer in parallel accumulation-serial fragmentation (PASEF) mode coupled to nano-flow liquid chromatography. Mass spectrometric raw data are analyzed with the DIA-NN workflow and the library-free mode.BBMRI-ERIC Section for Translational Surgical Oncology & Biobanking, Department of Surgerysamplehttps://www.uni-luebeck.de/ncch/lion.html
2.12c_IRST IRCCSITConfocal MicroscopeMultimodal advanced light microscopy and super-resolution microscopy, including live cell imaging and functional imaging for characterisation and validation of disease models and confocal microscopy.BBMRI-ERIC Istituto Scientifico Romagnolo per lo Studio dei Tumori "Dino Amatori" IRST IRCCScell lineshttps://www.irst.emr.it
2.18l_IRST IRCCSITWhole genome sequencingWhole genome sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologiesBBMRI-ERIC Istituto Scientifico Romagnolo per lo Studio dei Tumori "Dino Amatori" IRST IRCCSsamplehttps://www.irst.emr.it
2.18m_nCounter_IRSTITTarget RNA analysis Target RNA analysis (up to 800 targets per assay) using the nCounter Nanostring platformBBMRI-ERIC Istituto Scientifico Romagnolo per lo Studio dei Tumori "Dino Amatori" IRST IRCCSsamplehttps://www.irst.emr.it
2.3a_CliniMacs_IRSTITATMPs ProductionCell Factory for ATMPs production of nanoparticles for RNA delivery
BBMRI-ERIC Istituto Scientifico Romagnolo per lo Studio dei Tumori "Dino Amatori" IRST IRCCSNanoparticlehttps://www.irst.emr.it
2.2b_UNIMIB MedITLaser DissectionDigital pathology and Machine Learning -based image analysis; quality-controlled digitized whole-slide histological images of tumour and parallel normal tissue including annotated pathological and survival data BBMRI-ERIC UNIMIB_Pathologysamplehttps://en.unimib.it/
2.2c_UNIMIBITDigital PathologyDissector with laser acquisition of specific single cells or tissue areas for different uses, including omicsBBMRI-ERIC UNIMIB_Laser Dissectionsamplehttps://en.unimib.it/
2.19c+.24c_UNIMIB MedITProteomic_MetabolomicProteomics - MS/MS and MALDI Imaging Platform; Quantitative MS, quatitative Mass Spectrometry, Lipidomics and MetabolomicsBBMRI-ERIC UNIMIB_Proteomic_MetabolomicSamplehttps://en.unimib.it/
2.18kITWhole genome sequencingWhole genome sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologiesBBMRI-ERIC UNIMIB_Genomiccellhttps://en.unimib.it/
2.21a_UNIMIBITNanomedicineDevelopment of devices for: a) more efficient intracellular and controlled release of drugs, in particular co-delivery of antigens with immunostimulatory adjuvants , b) protection of antigen payloads and other bioactive molecules, c) increasing antigen bioavailability by encapsulation, adsorption or surface functionalization; d) decreased toxicity; efficient lymphatic drainage, enhanced permeability and retention effect at the tumour site BBMRI-ERIC UNIMIB_Nanomedicinesampleshttps://en.unimib.it/
2.18u_Single cell _LIEDDESingle cell mRNA sequencingSingle cell mRNA sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologiesBBMRI-ERIC Lübeck Institute of Experimental Dermatologysamplehttps://www.lied.uni-luebeck.de/home.html
2.18j_CNAGESWhole genome sequencingWhole genome sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologiesBBMRI-ERIC Centro Nacional de Análisis Genómico1Gbsequence datahttps://www.cnag.crg.eu/
2.18i_AOU-BO-MSSITTarget DNA/RNA sequencing: Oncomine Focus assayTarget DNA and RNA sequencing of hot spot genes, copy number gene variation and gene fusion (Oncomine Focus assay) of tumoral/non tumoral samples using next generation sequencing technologiesBBMRI-ERIC IRCCS Azienda Ospedaliero Universitaria di BolognaSamplehttps://www.aosp.bo.it/
2.18h_AOU-BO-MASSITTarget DNA Sequencing: custom gene panelsTarget sequencing of custom gene panels (hotspot genes) in tumoral and non tumoral samples using next generation sequencing technologiesBBMRI-ERIC IRCCS Azienda Ospedaliero Universitaria di BolognaSamplehttps://www.aosp.bo.it/
2.12b_BL2_NILPLConfocal Imaging2D/3D Imaging of living or fixed stained specimen by confocal microscopy equipped with 488, 543, 633 nm lasers, 4-100x objectives. BBMRI-ERIC Department of Biomedical Research / Translational Research LaboratoryCell linehttps://www.nil.gov.pl
2.35a_BL2_NILPLMolecular Modeling - DockingBinding analysis of compounds to specific proteins to understand anticancer molecular mechanisms of actionBBMRI-ERIC Department of Biomedical Research / Translational Research Laboratoryprojecthttps://www.nil.gov.pl
2.35b_BL2_NILPLMolecular Modeling - DynamicsLIgand-protein interactions and conformational analysis of proteins upon ligand bindingBBMRI-ERIC Department of Biomedical Research / Translational Research Laboratoryprojecthttps://www.nil.gov.pl
2.35c_BL2_NILPLMolecular Modelingt - ab initoADME (Absorption, Distribution, Metabolism, and Excretion) properties of tested compounds and evaluation of the conformational stability of moleculesBBMRI-ERIC Department of Biomedical Research / Translational Research Laboratoryprojectshttps://www.nil.gov.pl
2.28a_NMIPLFlow CytometryFlow cytometry technique (FACSCanto II flow cytometer) to detect apoptotic cells and mitochondrial membrane potential . Cell cycle analysis can be conducted.BBMRI-ERIC Department of Biomedical Research / Translational Research LaboratoryCellhttps://www.nil.gov.pl
2.17a_imag. radiomic_ATMAITRadiomicsProspective collection of diagnostic images (MR, CT, PT) of cancer patients and advanced imaging analysis (MR, CT, PT)BBMRI-ERIC ATMA Expert Centerdiagnostic image
2.17b_imag. radiomics_ATMAITRadiomicsAdvanced imaging analysis for early diagnosis and treatment response. It includes radiomic analysis and features selection to develop oncological personalized models.BBMRI-ERIC ATMA Expert Centerdiagnostics image
2.18o_UNIBO-Microbiome UnitIT16S rRNA microbiome sequencingMicrobial profiling using 16S ribosomal RNA (rRNA) next generation sequencing technologiesBBMRI-ERIC UNIBO-Microbiome Unitsamplehttps://dimec.unibo.it/it
2.18p_UNIBO Microbiome UnitITMetagenomic microbiome sequencingMicrobial profiling using shotgun metagenomic NGS technologiesBBMRI-ERIC UNIBO-Microbiome Unitsamplehttps://dimec.unibo.it/it
2.18n_UNIBO Microbiome UnitITMetatranscriptomic microbiome sequencingMicrobial profiling using shotgun metatranscriptomic next generation sequencing technologiesBBMRI-ERIC UNIBO-Microbiome Unitsamplehttps://dimec.unibo.it/it
2.28b_LTTA-FEITFlow CytometryMultiparametric immunophenotyping based on the use of specific fluorescent antibodies to measure targeted proteins expression within a cell population by flow cytometer, to investigate cancer severity, clonal expansion, proliferation, survival and differentiation in response to specific treatments of cancerous cells. Phenotypic characterization of the labeled cells as well as their sorting is available for further investigation. BBMRI-ERIC Laboratorio per le Tecnologie delle Terapie Avanzatesamplehttps://www.ltta.tecnopoloferrara.it
2.19b_LTTA-FEITLuminex multiplexingCytokines/chemochines quantitative multiparametric analysis, important for understanding the role of inflammation in cancer diseases. Luminex technology permits rapid and accurate analysis of panels of analytes (up to 50 at a time) in small sample volumes (25 µl) of serum/plasma or culture media. BBMRI-ERIC Laboratorio per le Tecnologie delle Terapie Avanzateprojecthttps://www.ltta.tecnopoloferrara.it
2.18f_UL BiobankPLWhole Genome SequencingWhole genome sequencing in tumoral and non tumoral samples using NGS technologiesBBMRI-ERIC University of Lodz - Biobank Lodz1 Gb of sequencing datahttps://www.biobank.uni.lodz.pl
2.18g_PanCan NGSITTarget DNA sequencing: actionable genesTarget sequencing of actionable genes in tumoral and non tumoral samples using next generation sequencing technologiesBBMRI-ERIC University Veronasamplehttps://www.univr.it
2.30a_Neuro.pathATChemosensitivity assaysChemosensitivity testing of tumor cells after treatment, helping to find best suitable drugs for respective patients. Proper concentrations of drugs are used, in order to make the results transferable for clinical use. Establishment of molecular tumor board is supported by introducing this technology.BBMRI-ERIC Institut of Pathology, Neuropathology and Molecular Pathologysamplehttps://www.i-med.ac.at/research-report/NeuroMolPathology.html
2.12a_Neuro.pathATHistology, Immunohistochemistry, Immunofluorescence, Cytology, Molecular pathology, diagnostics, confocal microscopyHistological assessment of respective samples, immunohistochemical and immunofluorescence staining for different biomarkers, confocal microscopy, morphometrics; morphological assessment support by A.I. tools available.BBMRI-ERIC Institut of Pathology, Neuropathology and Molecular Pathologysamplehttps://www.i-med.ac.at/research-report/NeuroMolPathology.html
2.18d_NCI-LT-Seq-DMLTTarget DNA sequencing: custom gene panelsTarget sequencing of custom gene panels in tumoral and non tumoral samples using Next Generation Sequencing technologiesBBMRI-ERIC Laboratory of Genetic Diagnosticsamplehttps://www.nvi.lt/laboratory-of-genetic-diagnostic/
2.18a_Pannoramic MIDICZDigital PathologyWhole-slide histological images of tumor and parallel normal tissues that are stored in Bank of Biological Materials including basic histopathological dataBBMRI-ERIC Department of Pathologysamplehttps://www.mou.cz/en/
2.18c_MultipotentiostatCZElectrochemical biosensorsConstruction of electrochemical biosensors for detection of DNA and/or RNA biomarkers: e.g. detection of HPV (genotyping HPV 16 and 18), SNPs, DNA methylations, miRNAs, lncRNA etc.BBMRI-ERIC RECAMOsamplehttps://www.mou.cz/en/
2.2a_Droplet Dig. PCRCZDigital PCRDetection of tumour biomarkers (miRNA, DNA mutations, CNVs) in tumour biopsies, cell free DNA, circulating tumor cells, etc. BBMRI-ERIC RECAMOsamplehttps://www.mou.cz/en/
2.18b_NextSeq 500CZTarget DNA sequencing: custom gene panelsTarget sequencing of custom gene panels in tumoral and non tumoral samples using next generation sequencing technologiesBBMRI-ERIC RECAMOsamplehttps://www.mou.cz/en/
2.18q_BRFAAGRTarget DNA sequencing: custom gene panelsTarget sequencing of custom gene panels in tumoral and non tumoral samples using next generation sequencing technologiesBBMRI-ERIC Biomedical Research Foundation of the Academy of Athenssamplehttp://www.bioacademy.gr/
2.18r_BRFAAGRTarget DNA sequencing: hereditary cancerTarget sequencing of genes involved in hereditary cancer using next generation sequencing technologiesBBMRI-ERIC Biomedical Research Foundation of the Academy of Athenssamplehttp://www.bioacademy.gr/
2.18s_BRFAAGRTarget DNA sequencing: hematological malignancies Target sequencing of genes involved in hematological malignancies using next generation sequencing technologiesBBMRI-ERIC Biomedical Research Foundation of the Academy of Athenssamplehttp://www.bioacademy.gr/
2.18t_BRFAAGRTarget DNA sequencing: TruSight Oncology 500Target sequencing of >500 genes relevant for solid tumors using next generation sequencing technologiesBBMRI-ERIC Biomedical Research Foundation of the Academy of Athenssamplehttp://www.bioacademy.gr/
2.16a_BRFAAGRPET-CT in micePreclinical PET/CT imaging with a state-of-the-art microPET/CT scanner and sophisticated image processing software for analyzing and quantifying PET and CT images of mice and rats. Appropriate racks for hosting animals for a long period of time are available. For more info please visit: https://www.infrafrontier.gr/whole-body-imaging-petctBBMRI-ERIC Biomedical Research Foundation of the Academy of Athenssamplehttp://www.bioacademy.gr/
2.16b_BRFAAGRPET-CT and exome-seq in micePreclinical PET-CT imaging and exome-sequencing in 1 mouse to reveal genetic variants that alter protein sequences. The Greek Genome Center is equipped with state-of-the-art Illumina sequencers, automated high throughput DNA/RNA extraction and liquid handling workstations and, finally, a high-performance computing cluster. BBMRI-ERIC Biomedical Research Foundation of the Academy of Athenssamplehttp://www.bioacademy.gr/
2.19d_BRFAAGRCyTOF analysisBRFAA mass cytometry unit provides a comprehensive full service, including data analysis, to evaluate cellular phenotype of human and mouse samples including: extracellular and intracellular markers of cell identity, cytokine production, signaling phenotypes and cellular division. We employ multiparametric panels of antigenic markers (>20 markers) with pre-made kits or customizable ones. Examples of such panels can be found at website https://www.brfaacytoflab.com/other-resources. BBMRI-ERIC Biomedical Research Foundation of the Academy of Athenssamplehttp://www.bioacademy.gr/
2.1a_IVM_CNRITIntravital microscopy (IVM) with fluorescent biosensors in mouse models of cancer Intravital microscopy (IVM) is the study of localization and dynamics of cellular processes relevant to cancer within the native tumor microenvironment. Services include housing of mice, surgical procedures necessary for installation of optical imaging windows adjacent to an tumors/organ of interest. Cancer model may include orthotopic/ectopic xenograft models in immunodeficient, syngeneic mouse tumor cell lines that can be genetically modified to express fluorescent reporters proteins for imaging purposes and genetic mouse models engineered to develop cancer. INFRAFRONTIER Consiglio Nazionale delle Ricerche projectshttp://www.ibbc.cnr.it
2.1b_Fluorescent biosensorsFIIntravital microscopy (IVM) with fluorescent biosensors in mouse models of cancer Intravital microscopy (IVM) is the study of localization and dynamics of cellular processes relevant to cancer within the native tumor microenvironment. Services include housing of mice, surgical procedures necessary for installation of optical imaging windows adjacent to an tumors/organ of interest. Cancer model may include orthotopic/ectopic xenograft models in immunodeficient, syngeneic mouse tumor cell lines that can be genetically modified to express fluorescent reporters proteins for imaging purposes and genetic mouse models engineered to develop cancer. INFRAFRONTIER University of Ouluprojectshttps://www.oulu.fi/en/university/faculties-and-units/biocenter-oulu
2.2_Dig. path_OuluFIDigital pathology and ML-based image analysis This service offers quality-controlled digitized whole-slide histological images of tumour and parallel normal tissue, including annotated pathological and survival data and dissector with laser acquisition.INFRAFRONTIER University of Ouluprojectshttps://www.oulu.fi/en/university/faculties-and-units/biocenter-oulu
2.1b_ISS CoreITImaging evaluation of murine model during cancer development and treatmentAdvanced and quantitative in vivo MRI and MRS to characterize rodents models of cancer during cancer development and following treatment. In particular: DWI with different diffusion models for functional characterization, molecular imaging with iron oxide or 19F nanoparticles for macrofages recruitment studies and quantitative 1H, 31P and 19F MRS for cancer model metabolic characterization.EATRIS Istituto Superiore di Sanità (ISS)MRI and MRS analyseshttps://www.iss.it/
2.28_Sort FCMITCell sorting of target populationCell sorting of target population (e.g. rare population,..), identified by flow or mass cytometry in human tissuesEATRIS Istituto Superiore di Sanità (ISS)hourhttps://www.iss.it/
2.49_Alonso MJESImmunocompetent murine models of cancer for antitumoral efficacy evaluationEvaluation of immunocompetent murine models of cancer for antitumoral efficacy, including effects on the tumor microenvironment. Expertise in (1) subcutaneous murine models of any type of cancer; (2) orthotopic immunocompetent models of fibrosarcoma or lung cancer, presenting a high infiltration of tumor-associated macrophages (i.e. MN/MCA or CMT167). EATRIS Health Research Institute of Santiago de Compostela (IDIS)Immunocompetent murine modelshttps://www.idisantiago.es/
2.50_Mol.Biol of CancerCZMonochrome Multiplex Real-Time qPCR Assay for telomere measurementMonochrome Multiplex Real-Time qPCR Assay for telomere measurement affected by incomplete DNA replication of a lagging strand or by genotoxic effect of environmental and intracellular DNA-damaging agents, including anticancer drugs. EATRIS Institute of Experimental Medicine CASexperiment/set of 100 sampleshttp://www.iem.cas.cz/en/institute/
2.26_Mol.Boiol of CancerCZComet assay (single-cell gel electrophoresis) for measuring DNA strand breaks and oxidative DNA damageThe comet assay (single-cell gel electrophoresis) is a simple method for measuring DNA strand breaks and oxidative DNA damage (Opattova, Horak et al. 2019). This method is applicable for human biomonitoring, testing novel chemicals for genotoxicity, environmental contamination with genotoxins and molecular epidemiology.EATRIS Institute of Experimental Medicine CASexperiment/set of 20 sampleshttp://www.iem.cas.cz/en/institute/
2.48_SDNITAssays for in vitro discovery researchCell cycle, apoptosis, proliferation and phosflow assay for in vitro discovery research (i.e. drug discovery, pathway analysis)EATRIS NETWORK OF NEUROSCIENCE AND NEUROREHABILITATION IRCCS samplehttps://www.reteneuroscienze.it/en/Istituto/irccs-sdn-istituto-di-ricerca/
2.16_Mol. imag. biomarkersESmicroPET/CT acquisition dynamic on murine modelThe service includes model generation (if possible in-house), animal handling, dynamic image acquisition and image post-processing. The radiotracer cost is included for radiotracers synthesized at our cyclotron or radiopharmacy facilities (18F-FDG, 18F-FLT, 18F-FMISO, 18F-choline,18F-DOPA, 68Ga-PSMA, 68Ga-DOTATOC). Unit of access includes animal housing and handling, radiotracer, and image processing after the experiment. Deliverables are: dynamic PET/CT images, full quantitative analysis reportEATRIS Health Research Institute of Santiago de Compostela (IDIS)1 animal (sample)https://www.idisantiago.es/
2.11_Molecul. imag. BiomarkersESmAbs labelling: Labelling of novel mAbs with 89Zr for PET imaging.Labelling of novel mAbs with 89Zr for PET imaging.EATRIS Health Research Institute of Santiago de Compostela (IDIS)mAbs labellinghttps://www.idisantiago.es/
2.19a_IBETPTAdvanced Mass Spectrometry for the Characterization of Molecular Tools The service includes Protein/peptide identification, Antibody Characterization using Multi-Attribute Method (MAM), Post-Translational Modifications Analysis (Glycosylation; Oxidation/Deamination, Glycan Identity…), High-throughput Quantitative Proteomics – SWATH analysis (untargeted; relative quantification), Quantitative Proteomics/peptidomics - MRM-HR (targeted) (relative and/or absolute quantitation) and Intact Protein Measurement.EATRIS iBET - Instituto de Biologia Experimental e TecnológicaSamples+Controls+Standardshttps://www.ibet.pt/
2.19b_IBETPTCharacterization of Molecular Tools - Aggregation and refolding (SEC and RP chromatography)Reverse phase (RP) and Size Exclusion Chromatography (SEC) to assess information on identity and purity attributes of protein-based biopharmaceuticals. SEC is applied for analysis of monomers, dimers, aggregates and degradation products according to their size. RP contributes to characterization of the product in terms of hydrophobicity.EATRIS iBET - Instituto de Biologia Experimental e TecnológicaSample (2 runs) + dedicated columnhttps://www.ibet.pt/
2.15b+2.46b_BrukerATPreclinical Magnetic Resonance ImagingMagnetic resonance imaging (MRI) of animal models, comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level. Used to monitor disease development and to test the efficacy of therapeutic treatments, to develop novel diagnostic tools etc. The service includes animal model preparation, provision of contrast agents, image data processing and analysis.EURO-BIOIMAGING Austrian BioImaginghourhttps://www.eurobioimaging.eu/nodes/austrian-bioimagingcmi
2.15c+2.46c_Vevo USATPreclinical UltraSound imagingUltraSound Imaging of animal models for visualizing and quantifying tumors, allowing staging, monitoring of therapeutic treatments, or guided injection of drugs. The use of targeting microbubbles as US contrast agents allows the visualization and quantification of specific tumor biomarkers as well. The service includes animal model preparation, probes, image data processing and analysis.EURO-BIOIMAGING Austrian BioImaginghourhttps://www.eurobioimaging.eu/nodes/austrian-bioimagingcmi
2.12a+2.33a+2.40a_live cellBGLive cell fluorescent imagingFluorescence microscopy imaging provided, both in spinning disk and confocal mode, on living cells. This also includes functional imaging to understand protein behavior. The facility specialises in tracking fast protein dynamics and interactions, particularly in DNA repair.EURO-BIOIMAGING Advanced Light Microscopy Node Sofia Bulgaria1 user projecthttps://www.eurobioimaging.eu/nodes/sofia-bioimaging-node---advanced-light-microscopy-node-sofia-bulgaria
2.12b+2.33b+2.40b_LM SuperCZLM Super-resolution and confocal microscopyA variety of different light microscopy methods are available individually or in combination. These methods include functional imaging with fluorescence microscopy, a particular focus on super-resolution imaging using specifcially STED and SIM to reach higher resolution compared to what is available using standard methods. These methods also include mesoscopic imaging via lightsheet, high-speed imaging, second-harmonics generation and other specialised light microscopy methodsEURO-BIOIMAGING Advanced Light and Electron Microscopy Node Prague CZHourhttps://www.eurobioimaging.eu/nodes/advanced-light-and-electron-microscopy-node-prague-cz
2.42d_LM Live CellCZLM Live cell QPIQuantitative Phase Imaging of living cells is a label-free light microscopy method to retrieve the phase information of light passing through the cell, and via this determine dry mass of the cell, its changes over time, and track cellular behavior over time. Due to the label free nature of the imaging, it can be applied to unstained samples, including patient biopsies. It is a gentle imaging method with little photodamage and can be applied to living cells over time.EURO-BIOIMAGING Advanced Light and Electron Microscopy Node Prague CZhourhttps://www.eurobioimaging.eu/nodes/advanced-light-and-electron-microscopy-node-prague-cz
2.14b_EM 3D MethodsCZEM 3D methodsVolume EM methods are used to gain insights into ultrastructural organisation of biological samples at a sub-cellular level over a 3D volume of tissue. This can be applied to different tissues samples using various sample preparation methods. Specifically offered volume EM methods are serial-section TEM, serial blockface SEM, and FIB-SEM (Focussed Ion Beam Scanning Electron Microscopy).EURO-BIOIMAGING Advanced Light and Electron Microscopy Node Prague CZhourhttps://www.eurobioimaging.eu/nodes/advanced-light-and-electron-microscopy-node-prague-cz
2.14c_EM RoutineCZEM routine TEMStandard transmission electron microscopy methods to gain ultrastructural insights into subcellular organisation. The service includes different sample preparation methods, such as cryo-EM and resin-EM, and a variety of labelling methods to localise specific molecules within a sample.EURO-BIOIMAGING Advanced Light and Electron Microscopy Node Prague CZhourhttps://www.eurobioimaging.eu/nodes/advanced-light-and-electron-microscopy-node-prague-cz
2.12c+2.33c+2.40c_CELLIMCZFluorescence MicroscopyService provides access and support for a variety of light microscopy methods, specifically functional imaging with fluorescence microscopy, super-resolution imaging via single molecule localisation microscopy (SMLM) and structured illumination microscopy (SIM), mesoscopic imaging via lightsheet, expansion microscopy and other specialised light microscopy methodsEURO-BIOIMAGING Advanced Light Microscopy and Medical Imaging Node Brno CZ hourhttps://www.eurobioimaging.eu/nodes/advanced-light-microscopy-and-medical-imaging-node-brno-cz
2.15d+2.46d_AlbiraCZPreclinical PET-CT and SPECT-CT imagingPositron Emission Tomography (PET) and Single Photon Emission Tomography (SPECT) Imaging of animal models, for monitoring function and metabolism. PET and SPECT imaging can be performed as single modality acquisition but can also be used in combination with CT, for precise spatial localization of the PET/SPECT signals within the subject. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. EURO-BIOIMAGING Centre for Advanced Preclinical Imaginghourhttps://www.eurobioimaging.eu/nodes/center-for-advanced-preclinical-imaging-capi
2.15e+2.46e_Xtreme OpticalCZIn vivo optical imagingIn vivo preclinical optical Imaging, for visualizing and characterizing tumors and for imaging guided surgery. Fluorescence, bioluminescence and other optical imaging techniques are available. A variety of bioluminescent and/or fluorescent probes can be used, each targeted to a specific tissue or molecular or cellular event. Animal model preparation, probes and image data processing and analysis are included in the service.EURO-BIOIMAGING Centre for Advanced Preclinical Imaginghourhttps://www.eurobioimaging.eu/nodes/center-for-advanced-preclinical-imaging-capi
2.15f+2.46f_VEVO USCZPreclinical UltraSound imagingUltraSound Imaging of animal models for visualizing and quantifying tumors, allowing staging, monitoring of therapeutic treatments, or guided injection of drugs. The use of targeting microbubbles as US contrast agents allows the visualization and quantification of specific tumor biomarkers as well. The service includes animal model preparation, probes, image data processing and analysis.EURO-BIOIMAGING Centre for Advanced Preclinical Imaginghourhttps://www.eurobioimaging.eu/nodes/center-for-advanced-preclinical-imaging-capi
2.15g+2.46g_7T MRICZPreclinical Magnetic Resonance ImagingMagnetic resonance imaging (MRI) of animal models, comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level. Used to monitor disease development and to test the efficacy of therapeutic treatments, to develop novel diagnostic tools etc. The service includes animal model preparation, provision of contrast agents (with possibility of custom synthesis of specific targeting and/or theranostic probes if needed), image data processing and analysis.EURO-BIOIMAGING Centre for Advanced Preclinical Imagingcomplete accesshttps://www.eurobioimaging.eu/nodes/center-for-advanced-preclinical-imaging-capi
2.12d+2.33d+2.40d+2.13a+2.41a_EMBLDEFluorescence MicroscopyDifferent fluorescence microscopy methods are offered including expertise in project development and image analysis. The offered methods include advanced super-resolution microscopy techniques such as single molecule localisation, STED, MINFLUX, live cell imaging, mesoscopic imaging via lightsheet, functional imaging, intravital microscopy, high-throughput imaging and correlative techniques.EURO-BIOIMAGING EMBL-Nodehourhttps://www.eurobioimaging.eu/nodes/advanced-light-microscopy-facility-embl
2.14d_EMBLDEElectron MicroscopyDifferent electron microscopy methods are offered, including correlative light and electron microscopy methods (CLEM), a variety of sample preparations, including cryo-EM methods, labelling methods to localise proteins within EM samples, and volume EM methods for 3D ultrastructural investigations. VolumeEM methods offered are serial section TEM, Serial Blockface-SEM, FIB-SEMEURO-BIOIMAGING EMBL-Nodehourhttps://www.eurobioimaging.eu/nodes/advanced-light-microscopy-facility-embl
2.12f+2.33f+2.40f_Super-resolution mic.FISuper-resolution MicroscopySuper-resolution methods offered to gain increased resolution insights using fluorescence microscopy beyond what is gathered using standard fluorescence methods. The offered super-resolution approaches are single-molecule localisation microscopy (PALM, STORM), structured illumination microscopy (SIM), and STED, including needed sample preparation and analysis steps.EURO-BIOIMAGING Finnish Advanced Microscopy Nodehourhttps://www.eurobioimaging.eu/nodes/finnish-alm-node---advanced-light-microscopy-finnish-node
2.14e_Electron Mocro.FIElectron MicroscopyDifferent electron microscopy methods are offered, including labelling methods to localise proteins within EM samples, and volume EM methods for 3D ultrastructural investigations. VolumeEM methods offered are Array tomography, serial section TEM, Serial Blockface-SEM.EURO-BIOIMAGING Finnish Advanced Microscopy Nodehourhttps://www.eurobioimaging.eu/nodes/finnish-alm-node---advanced-light-microscopy-finnish-node
2.14f_CLEMFICorrelative Light and Electron MicroscopyCorrelative Light and Electron Microscopy combines and correlates light and electron microscopy imaging of the same sample to achieve a higher level of understanding of the sample than possible with either technique in isolation. The facilities offer expertise starting from imaging of living cells using fluorescence microscopy, up to correlation with different EM methods for ultrastructural localisation of correlated proteins of interest.EURO-BIOIMAGING Finnish Advanced Microscopy Nodehourhttps://www.eurobioimaging.eu/nodes/finnish-alm-node---advanced-light-microscopy-finnish-node
2.13b+2.41b_Mesoscopic ImagingFIMesoscopic ImagingMesoscopic Imaging allows imaging of larger samples, such as tissues, organoids, and small animals at high resolution. This can be combined with cleared and expanded tissues. The specific offered methods are lightsheet microscopy and optical projection tomographyEURO-BIOIMAGING Finnish Advanced Microscopy Nodehourhttps://www.eurobioimaging.eu/nodes/finnish-alm-node---advanced-light-microscopy-finnish-node
2.15h+2.46h_Fibl-MEGFIMagnetoEncephaloGraphy & MRIMagnetoencephalography (MEG) measures noninvasively minute magnetic fields produced by neuronal activity of the brain. MEG (as a standalone technique or coupled with MRI) can be used for mapping functionally important regions in the brain prior to surgery and for detecting functional impairment caused by brain tumors. EURO-BIOIMAGING Finnish BioMedical Imaging Nodecomplete accesshttps://www.eurobioimaging.eu/nodes/finnish-biomedical-imaging-node
2.17a_PET/CTFIPreclinical PET-CT ImagingPositron Emission Tomography (PET) Imaging of animal models, for monitoring function and metabolism. PET imaging can be performed as single modality acquisition but can also be used in combination with CT, for precise spatial localization of the PET signals within the subject. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. EURO-BIOIMAGING Finnish BioMedical Imaging Nodecomplete accesshttps://www.eurobioimaging.eu/nodes/finnish-biomedical-imaging-node
2.17b+2.15i+2.46i_SPECT/CTFIPreclinical SPECT-CT ImagingSingle Photon Emission Tomography (SPECT) Imaging of animal models, for monitoring function and metabolism. SPECT imaging can be performed as single modality acquisition but can also be used in combination with CT, for precise spatial localization of the SPECT signals within the subject. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. EURO-BIOIMAGING Finnish BioMedical Imaging Nodecomplete accesshttps://www.eurobioimaging.eu/nodes/finnish-biomedical-imaging-node
2.12e+2.33e+2.40e_MultimodalFIMultimodal Advanced Light MicroscopyAdvanced fluorescence microscopy methods include live cell imaging, functional imaging to investigate protein dynamics and interactions (FRET, FRAP, FLIM, FCCS), second harmonics generation, total internal reflection fluorecence microscopy (TIRF), and correlative techniques.EURO-BIOIMAGING Finnish Advanced Microscopy Nodehourhttps://www.eurobioimaging.eu/nodes/finnish-alm-node---advanced-light-microscopy-finnish-node
2.12g+2.33g+2.40g_Reg.LightFRRegular Light MicroscopyLight Microscopy methods are offered including fluorescence and label-free techniques, functional imaging, confocal and deconvolution microscopy, to investigate cellular and sub-cellular behavior and localisations. These methods can be applied also correlatively with high-end fluorescence microscopy and electron microscopy methodsEURO-BIOIMAGING French BioImaging Nodehourhttps://www.eurobioimaging.eu/nodes/french-bioimaging-node
2.12h+2.33h+2.40hFRHigh and super-resolution microscopySuper-resolution methods including single-molecule localisation microscopy (PALM, STORM), SIM, STED, as well as methods for high resolution imaging on standard systems such as image scanning microscopy on confocal and spinning disk systems. These methods improve the resolution for the gathered data from an around 2-fold increase to very high resolution on the nm level.EURO-BIOIMAGING French BioImaging Nodehourhttps://www.eurobioimaging.eu/nodes/french-bioimaging-node
2.13c+2.41c_Longitudinal IntervalFRLongitudinal intravital microscopyIntravital imaging covers a range of microscopy modalities used for the long term imaging of living animal models (e.g. zebrafish larvae, mice, rats), in various organs or tissues, or as a whole for smaller models. These methods allow tracking of tissue, cellular, and even sub-cellular, behavior in a larger context of either an organ or organoid, or a whole animal. In whole animals this is done using windows, such as cranial windows and abdominal windows, to allow access to the inside of the animalEURO-BIOIMAGING French BioImaging Nodehourhttps://www.eurobioimaging.eu/nodes/french-bioimaging-node
2.13d+2.41d_Lattice Light SheetFRLattice Lightsheet MicroscopyLattice Light Sheet Microscopy is a special lightsheet microscope where the excitation plane is very thin (<1 µm) and uniformly flat over typical cellular dimensions. The main advantages of LLSM are its intrinsic optical sectioning, fast imaging (up to 300 frames per second), low photobleaching and phototoxicity, and sub-micrometer spatial resolution over long light sheets (10–100 µm).EURO-BIOIMAGING French BioImaging Nodehourhttps://www.eurobioimaging.eu/nodes/french-bioimaging-node
2.14g_Reg. EM/TEMFRElectron MicroscopyTransmission Electron Microscopy and Scanning Electron Microscopy on samples prepared with different methods, such as resin-EM and cryo-EM. This also includes support for different sample labelling methods to localise proteins in the sample.EURO-BIOIMAGING French BioImaging Nodecomplete accesshttps://www.eurobioimaging.eu/nodes/french-bioimaging-node
2.12i+2.33i+2.40iFRHigh-end confocal MicroscopyHigh-end and specialised confocal microscopy methods to achieve high-resolution and fast imaging with 3D resolution, including in living and fixed cellsEURO-BIOIMAGING French BioImaging Nodecomplete accesshttps://www.eurobioimaging.eu/nodes/french-bioimaging-node
2.14h_CLEMFRAdvanced Electron MicroscopyAdvanced Electron Microscopy methods, including cryo-EM sample presentation, Correlative Light and Electron Microscopy, and volume EM methods for 3D ultrastructural investigations in a larger cellular and tissue context. The available volumeEM methods are FIB-SEM, serial blockface-SEM, and serial section TEM. This also includes Correlative X-ray, light and electron microscopy imagingEURO-BIOIMAGING French BioImaging Nodehourhttps://www.eurobioimaging.eu/nodes/french-bioimaging-node
2.12j+2.33j+2.40j_UNIDEBHUAdvanced Light MicroscopyAdvanced Light Microscopy methods, particularly fluorescence imaging methods with a specific focus on functional imaging methods to study protein interactions and dynamics (FRET, FRAP, FLIM, FCCS), as well as high-throughput imaging and light-sheet microscopyEURO-BIOIMAGING Cellular Imaging Hungary Nodecomplete accesshttps://www.eurobioimaging.eu/nodes/cellular-imaging-hungary
2.12k+2.33k+2.40k_SemmelweisHUSuper-resolutionA range of microscopy techniques used to achieve a resolution that is higher than that of conventional, diffraction-limited microscopy, particularly single-molecule localisation microscopy (SMLM) and STED.EURO-BIOIMAGING Cellular Imaging Hungary Nodecomplet accesshttps://www.eurobioimaging.eu/nodes/cellular-imaging-hungary
2.14i_BRC CILABHUAdvanced Light and correlative imaging methodsAdvanced Light Microscopy methods, including super-resolution and functional imaging, in combination and correlation with Electron Microscopy approaches and methods for sample characterisation.EURO-BIOIMAGING Cellular Imaging Hungary Nodecomplete accesshttps://www.eurobioimaging.eu/nodes/cellular-imaging-hungary
2.12l+2.33l+2.40l_FEMTOHUTwo-photon MicroscopyMultiphoton microscopy - most commonly in the form of two-photon microscopy - is a fluorescence imaging technique that allows observation of living tissue up to about one millimeter in depth. EURO-BIOIMAGING Cellular Imaging Hungary Nodecomplete accesshttps://www.eurobioimaging.eu/nodes/cellular-imaging-hungary
2.15j+2.46jHUPreclinical PET Imaging and AutoradiographyPositron Emission Tomography (PET) Imaging of animal models, for monitoring function and metabolism. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. EURO-BIOIMAGING Medical and Preclinical Imaging Hungarycomplete accesshttps://www.eurobioimaging.eu/nodes/medical-and-preclinical-imaging-hungary
2.17c_SCANOMEDHUPreclinical PET-CT and SPECT-CT imagingPositron Emission Tomography (PET) and Single Photon Emission Tomography (SPECT) Imaging of animal models, for monitoring function and metabolism. PET and SPECT imaging can be performed as single modality acquisition but can also be used in combination with CT, for precise spatial localization of the PET/SPECT signals within the subject. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. EURO-BIOIMAGING Medical and Preclinical Imaging Hungarycomplete accesshttps://www.eurobioimaging.eu/nodes/medical-and-preclinical-imaging-hungary
2.15k+2.46k+2.17d_SemmelweisHUPreclinical PET-CT and SPECT-CT imagingPositron Emission Tomography (PET) and Single Photon Emission Tomography (SPECT) Imaging of animal models, for monitoring function and metabolism. PET and SPECT imaging can be performed as single modality acquisition but can also be used in combination with CT, for precise spatial localization of the PET/SPECT signals within the subject. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. EURO-BIOIMAGING Medical and Preclinical Imaging Hungarycomplete accesshttps://www.eurobioimaging.eu/nodes/medical-and-preclinical-imaging-hungary
2.15l+2.46l_UP,SzRCHUIn vivo Optical ImagingIn vivo preclinical optical Imaging, for visualizing and characterizing tumors and for imaging guided surgery. Fluorescence, bioluminescence and other optical imaging techniques are available. A variety of bioluminescent and/or fluorescent probes can be used, each targeted to a specific tissue or molecular or cellular event. Animal model preparation, probes and image data processing and analysis are included in the service.EURO-BIOIMAGING Medical and Preclinical Imaging Hungarycomplete accesshttps://www.eurobioimaging.eu/nodes/medical-and-preclinical-imaging-hungary
2.15m+2.46m_BiospecILPreclinical Magnetic Resonance ImagingMagnetic resonance imaging (MRI) of animal models, comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level. Used to monitor disease development and to test the efficacy of therapeutic treatments, to develop novel diagnostic tools etc. The service includes animal model preparation, provision of contrast agents (with possibility of custom synthesis of specific targeting and/or theranostic probes if needed), image data processing and analysis.EURO-BIOIMAGING Israel BioImagingShift (4 hours)https://www.eurobioimaging.eu/nodes/israel-bioimaging
2.12m+2.33m+2.40m_UltramicroILSuper-resolutionA range of microscopy techniques used to achieve a resolution that is higher than that of conventional, diffraction-limited microscopy, specifically as single-molecule localisation microscopy (SMLM) and STED.EURO-BIOIMAGING Israel BioImaginghour
2.19a,2.42a_VBCFATlow-resolution Mass Spectrometry based ImagingSurface analysis techniques which are based on desorption and ionization of molecules followed by their subsequent MS data recording. After collecting a mass spectrum at one spot, the sample is moved to the neighboring spot, and the mass spectrum is sampled again, until the entire sample surface is scannedEURO-BIOIMAGING Austrian BioImagingHourhttps://www.eurobioimaging.eu/nodes/austrian-bioimagingcmi
2.32a_HCSILHigh-content screeningUsing automated cell manipulations and microscopy platforms, high-content screening can range from entire genomes screens and high-content analysis, to slide scanning of a large number of tissue biopsy sections. HCS techniques rely on fully automated widefield or confocal microscopy systems which facilitate the capture of thousands of images, often in multi-well plates or on slides. EURO-BIOIMAGING Israel BioImagingdayhttps://www.eurobioimaging.eu/nodes/israel-bioimaging
2.15n+2.46n_in vivo optic.imag.ILIn vivo Optical ImagingIn vivo preclinical optical Imaging, for visualizing and characterizing tumors and for imaging guided surgery. Fluorescence, bioluminescence and other optical imaging techniques are available. A variety of bioluminescent and/or fluorescent probes can be used, each targeted to a specific tissue or molecular or cellular event. This service makes use of an Olympus optical system. Animal model preparation, probes and image data processing and analysis are included in the service.EURO-BIOIMAGING Israel BioImaginghourhttps://www.eurobioimaging.eu/nodes/israel-bioimaging
2.15o+2.46o_in vivo optic. imag.ILIn vivo Optical ImagingIn vivo preclinical optical Imaging, for visualizing and characterizing tumors and for imaging guided surgery. Fluorescence, bioluminescence and other optical imaging techniques are available. A variety of bioluminescent and/or fluorescent probes can be used, each targeted to a specific tissue or molecular or cellular event. This service makes use of a Spektrum IVIS optical system. Animal model preparation, probes and image data processing and analysis are included in the service.EURO-BIOIMAGING Israel BioImaginghourhttps://www.eurobioimaging.eu/nodes/israel-bioimaging
2.15p+2.46p_in vivo optic.imagILIn vivo Optical ImagingIn vivo preclinical optical Imaging, for visualizing and characterizing tumors and for imaging guided surgery. Fluorescence, bioluminescence and other optical imaging techniques are available. A variety of bioluminescent and/or fluorescent probes can be used, each targeted to a specific tissue or molecular or cellular event. This service makes use of an IVIS-KINETIC optical system. Animal model preparation, probes and image data processing and analysis are included in the service.EURO-BIOIMAGING Israel BioImaginghourhttps://www.eurobioimaging.eu/nodes/israel-bioimaging
2.12n+2.33n+2.40n_LMITLight MicroscopyLight Microscopy methods are offered including fluorescence and label-free techniques, multiphoton and volumetric imaging, non-linear microscopy, functional imaging for protein dynamics and interactions, in-flow microscopy, large sample imaging in cleared and expanded samplesEURO-BIOIMAGING Advanced Light Microscopy Italian Nodecomplete accesshttps://www.eurobioimaging.eu/nodes/advanced-light-microscopy-italian-node
2.12o+2.33o+2.40o_SRITSuper-resolution MicroscopySingle-molecular localisation microscopy (PALM, STORM) including both in 2D and 3D, STED, and image scanning microscopy to achieve higher resolution images compared to standard diffraction-limited microscopyEURO-BIOIMAGING Advanced Light Microscopy Italian Nodecomplete accesshttps://www.eurobioimaging.eu/nodes/advanced-light-microscopy-italian-node
2.14j_EMITElectron MicroscopyDifferent electron microscopy methods, particularly as CLEM in correlation with light microscopy. The offered methods include transmission, scanning, cryo, and volume EM, such as FIB-SEM and serial section TEM.EURO-BIOIMAGING Advanced Light Microscopy Italian Nodecomplete accesshttps://www.eurobioimaging.eu/nodes/advanced-light-microscopy-italian-node
2.32b_HTIITHigh-throughput ImagingUsing automated cell manipulations and microscopy platforms, high-content screening can range from entire genomes screens and high-content analysis, to slide scanning of a large number of tissue biopsy sections. HCS techniques rely on fully automated widefield or confocal microscopy systems which facilitate the capture of thousands of images, often in multi-well plates or on slides. EURO-BIOIMAGING Advanced Light Microscopy Italian Nodecomplete accesshttps://www.eurobioimaging.eu/nodes/advanced-light-microscopy-italian-node
2.15q+2.46q_micro PETITPreclinical PET-CT imagingPositron Emission Tomography (PET) Imaging of animal models, for monitoring function and metabolism. PET imaging can be performed as single modality acquisition but can also be used in combination with CT, for precise spatial localization of the PET signals within the subject. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. EURO-BIOIMAGING Digital Imaging Multimodal Platform Neuromed - DIMP NEUROMEDcomplete accesshttps://www.eurobioimaging.eu/nodes/digital-imaging-multimodal-platform-neuromed---dimp-neuromed
2.19b+2.42b_Brucker FTICRATHigh-resolution Mass Spectrometry based ImagingSurface analysis techniques which are based on desorption and ionization of molecules followed by their subsequent MS data recording. After collecting a mass spectrum at one spot, the sample is moved to the neighboring spot, and the mass spectrum is sampled again, until the entire sample surface is scannedEURO-BIOIMAGING Austrian BioImagingHourhttps://www.eurobioimaging.eu/nodes/austrian-bioimagingcmi
2.12p+2.33p+2.40pITLight MicroscopyFoundational light microscopy tools and sample preparation for immunohistochemistry on animal samples provided in concert with other preclinical imaging measuresEURO-BIOIMAGING Digital Imaging Multimodal Platform Neuromed - DIMP NEUROMEDcomplete accesshttps://www.eurobioimaging.eu/nodes/digital-imaging-multimodal-platform-neuromed---dimp-neuromed
2.15r+2.46r_Population imag.ITPopulation imagingApplication of advanced image analysis tools to large numbers of medical images related to population cohorts for the identification and monitoring of specific imaging biomarkers which can provide information about diseases at the population level, allowing early diagnosis, early identification of people at risk and preventive measures.EURO-BIOIMAGING Digital Imaging Multimodal Platform Neuromed - DIMP NEUROMEDcomplete accesshttps://www.eurobioimaging.eu/nodes/digital-imaging-multimodal-platform-neuromed---dimp-neuromed
2.17e_Phase ContrastITPhase Contrast X-ray ImagingIn respect to other x-rays based imaging techniques, Phase Contrast X-ray Imaging (PCI) is useful for imaging soft tissues that do not possess large variations in X-ray absorption properties. Phase Contrast Imaging can also be combined with tomographic techniques to obtain 3D reconstruction. Image data processing and analysis included in the service.EURO-BIOIMAGING Phase Contrast Imaging Flagship Node TriesteHourhttps://www.eurobioimaging.eu/nodes/phase-contrast-imaging-flagship-node-trieste
2.15s+2.46s_MRIITPreclinical Magnetic Resonance ImagingMagnetic resonance imaging (MRI) of animal models, comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level. Used to monitor disease development and to test the efficacy of therapeutic treatments, to develop novel diagnostic tools etc. The service includes animal model preparation, provision of contrast agents (with possibility of custom synthesis of specific targeting and/or theranostic probes if needed), image data processing and analysis.EURO-BIOIMAGING MultiModal Molecular Imaging Italian Nodecomplete accesshttps://www.eurobioimaging.eu/nodes/molecular-imaging-italian-node
2.15t+2.46t_PET/SPECTITPreclinical PET-CT and SPECT-CT imagingPositron Emission Tomography (PET) and Single Photon Emission Tomography (SPECT) Imaging of animal models, for monitoring function and metabolism. PET and SPECT imaging can be performed as single modality acquisition but can also be used in combination with CT, for precise spatial localization of the PET/SPECT signals within the subject. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. EURO-BIOIMAGING MultiModal Molecular Imaging Italian Nodecomplete accesshttps://www.eurobioimaging.eu/nodes/molecular-imaging-italian-node
2.17f_CT_MMMI 3ITPreclinical X-Rays Computed TomographyX-rays Computed Tomography (CT) makes use of X-ray images (projections) taken from different angles and image reconstruction techniques to produce high resolution anatomic cross-sectional (tomographic) 3D images. CT contrast agents (usually based on Iodine, Barium, Gold) can be used to enhance the contrast. Preclinical microCT systems can be stand alone or integrated with other modalities like optical or nuclear medicine imaging in order to perform multimodal imaging.EURO-BIOIMAGING MultiModal Molecular Imaging Italian Nodecomplete accesshttps://www.eurobioimaging.eu/nodes/molecular-imaging-italian-node
2.34aITPreclinical Photocoustic imagingPhotoacoustic Imaging (PAI) of animal models, combining the advantages of optical and US imaging technologies. The US signal makes it possible to achieve an increase of both spatial resolution (micrometric) and penetration depth with respect to optical imaging and reduces scattering phenomena. In comparison with US a wider range of molecules can be used as PAI contrast agents. PAI allows to get 3D-high resolution images of soft tissues which provide anatomical, functional, and molecular information. The service includes animal model preparation, probes and image data processing and analysis.EURO-BIOIMAGING MultiModal Molecular Imaging Italian Nodecomplete accesshttps://www.eurobioimaging.eu/nodes/molecular-imaging-italian-node
2.14k_Light fluorescenceNLLight and Fluorescence MicroscopyLight Microscopy methods, including fluorescence microscopy in deconvolution widefield, confocal and spinning disk mode, functional imaging such as FLIM, FRET, FRAP and FCCS to measure protein dynamics and interactionsEURO-BIOIMAGING Facility of MultiModal Imaging, AMMI Maastrichthourhttps://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht
2.12q+2.33q+2.40q_Spectral fluor.NLMultispectral ImagingMulitspectral Imaging allows the simultaneous detection mulitple biomarkers in tissues at the same time within a sample, including when they are co-localised. This is especially applicable for tissue section and biopsies where samples are rare and individual can be simultaneously stained with many markers to extract maximum information from individual samples.EURO-BIOIMAGING Facility of MultiModal Imaging, AMMI Maastrichthourhttps://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht
2.12r+2.33r+2.40rNLSuper-resolution MicroscopySuper-resolution microscopy, specifically in the form of STED microscopy, for increased resolution compared to standard diffraction-limited microscopyEURO-BIOIMAGING Facility of MultiModal Imaging, AMMI Maastrichthourhttps://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht
2.42c_ATI XRayLabATMicro X-ray Fluorescence SpectrometryMicro-X-Ray Fluorescence Spectrometry (XRF) allows the qualitative analysis of the elemental composition of solid samples. The detected signal results from the emission of element-characteristic fluorescence X-rays from a sample bombarded with high-energy X-raysEURO-BIOIMAGING Facility of MultiModal Imaging, AMMI MaastrichtWeekhttps://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht
2.13e+2.41e_Light fluor.NLTwo-photon microscopyMultiphoton microscopy - most commonly in the form of two-photon microscopy - is a fluorescence imaging technique that allows observation of living tissue up to about one millimeter in depth. EURO-BIOIMAGING Facility of MultiModal Imaging, AMMI Maastrichthourhttps://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht
2.14lNLTransmission Electron MicroscopyIn transmission electron microscopy (TEM) the accelerated beam of electrons is transmitted through the sample and collected to form an image. To achieve this the specimen is usually sectioned into ultrathin sections less than 100 nm thick. Different methods for sample preparation are availableEURO-BIOIMAGING Facility of MultiModal Imaging, AMMI Maastrichthourhttps://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht
2.14mNLCryo-Transmission Electron MicroscopyIn transmission electron microscopy (TEM) the accelerated beam of electrons is transmitted through the sample and collected to form an image. To achieve this the specimen is usually sectioned into ultrathin sections less than 100 nm thick. Here specifically samples are cryo-fixed, either through plunge freezing for very small samples or via high-pressure freezingEURO-BIOIMAGING Facility of MultiModal Imaging, AMMI Maastrichthourhttps://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht
2.14nNLScanning Electron MicroscopyScanning electron microscopy describes a mode of electron microscopy in which the beam of accelerated electrons is scanned along the surface of the sample to gather insights into the surface structure at nanometer resolution. This can be applied to either whole samples or sections.EURO-BIOIMAGING Facility of MultiModal Imaging, AMMI Maastrichthourhttps://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht
2.14o_DualBeamNLDual Beam Scanning Electron MicroscopyScanning electron microscope with a dual beam set up allowing for faster data acquisition in scanning electron microscopy mode.EURO-BIOIMAGING Facility of MultiModal Imaging, AMMI Maastrichthourhttps://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht
2.19c+2.42e_SOLARIXNLMass-Spectrometry based Imaging using SOLARIXSurface analysis techniques which are based on desorption and ionization of molecules followed by their subsequent MS data recording. After collecting a mass spectrum at one spot, the sample is moved to the neighboring spot, and the mass spectrum is sampled again, until the entire sample surface is scanned. Here performed via SOLARIX.EURO-BIOIMAGING Facility of MultiModal Imaging, AMMI MaastrichtHourhttps://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht
2.19d+2.42f_RAPIFLEXNLMass-Spectrometry based Imaging RAPIFLEXSurface analysis techniques which are based on desorption and ionization of molecules followed by their subsequent MS data recording. After collecting a mass spectrum at one spot, the sample is moved to the neighboring spot, and the mass spectrum is sampled again, until the entire sample surface is scanned. Here performed via SOLARIX.EURO-BIOIMAGING Facility of MultiModal Imaging, AMMI MaastrichtHourhttps://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht
2.32cNLMass-Spectrometry based Imaging using HTXSurface analysis techniques which are based on desorption and ionization of molecules followed by their subsequent MS data recording. After collecting a mass spectrum at one spot, the sample is moved to the neighboring spot, and the mass spectrum is sampled again, until the entire sample surface is scanned. Here performed via HTXEURO-BIOIMAGING Facility of MultiModal Imaging, AMMI Maastrichthourhttps://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht
2.13f_LEICANLLight MicroscopyLight Microscopy methods, specifcally label-free techniques, to investigate cellular and sub-cellular behavior and localisationsEURO-BIOIMAGING Facility of MultiModal Imaging, AMMI Maastrichthourhttps://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht
2.15u+2.46u_PET/SPECTNLPreclinical PET and SPECT imagingPositron Emission Tomography (PET) and Single Photon Emission Tomography (SPECT) Imaging of animal models, for monitoring function and metabolism. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. EURO-BIOIMAGING Facility of MultiModal Imaging, AMMI Maastrichtcomplete accesshttps://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht
2.14a_HREMATHigh-resolution Episcopic microscopyHigh-resolution Episcopic microscopy or Serial block face fluorescence imaging is a method used to generate 3-dimensional (3D) reconstruction of a sample via serial image acquisition, here on a microtome, using light microscopy imaging techniques.EURO-BIOIMAGING Austrian BioImaginghourhttps://www.eurobioimaging.eu/nodes/austrian-bioimagingcmi
2.17f_CTNLPreclinical X-Rays Computed TomographyX-rays Computed Tomography (CT) on tissues, organs or living animal models. CT contrast agents (usually based on Iodine, Barium, Gold) can be used to enhance the contrast. Preclinical microCT systems can be stand alone or integrated with other modalities like optical or nuclear medicine imaging in order to perform multimodal imaging. The service includes animal model prepartion, contrast agents if needed, image data processing and analysis.EURO-BIOIMAGING Facility of MultiModal Imaging, AMMI Maastrichtcomplete accesshttps://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht
2.15v+2.46v_in vivo optic.imagNLIn vivo Optical ImagingIn vivo preclinical optical Imaging, for visualizing and characterizing tumors and for imaging guided surgery. Fluorescence, bioluminescence and other optical imaging techniques are available. A variety of bioluminescent and/or fluorescent probes can be used, each targeted to a specific tissue or molecular or cellular event. Animal model preparation, probes and image data processing and analysis are included in the service.EURO-BIOIMAGING Facility of MultiModal Imaging, AMMI Maastrichtcomplete accesshttps://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht
2.14p_Electron microsc.NLElectron Microscopy Sample preparation supportSupport with both the selection of the most appropriate sample preparation technique for different Electron Microscopy investigations, trouble-shooting of protocols and practical sample preparation supportEURO-BIOIMAGING Facility of MultiModal Imaging, AMMI Maastrichthourhttps://www.eurobioimaging.eu/nodes/facility-of-excellence-in-imaging---alm-and-molecular-imaging-node-maastricht
2.14q_Dutch CLEMNLCorrelative Light and Electron MicroscopyA wide variety of electron microscopy methods, including correlative light and electron microscopy (CLEM) is available. The EM methods include different vEM modalities, such as Array Tomography, FIB-SEM, serial blockface SEM and serial section TEM. Different methods for sample prepartion inlcuding chemical and cryo-fixation, and labelling of proteins of interest in EM sample are available. The service also includes Elemental analysis via EDX.EURO-BIOIMAGING Dutch CLEM Nodecomplete accesshttps://www.eurobioimaging.eu/nodes/correlative-light-microscopy-dutch-flagship-node
2.15w+2.46wNLHigh Field Magnetic Resonance Imaging (human)Human high field Magnetic resonance imaging (MRI), comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level. MRI scanners at high magnetic fields have the advantage to significantly increase the sensitivity and thus improve the image resolution and scan duration. This service is provided on a 7T scanner.EURO-BIOIMAGING Dutch High Field MR Hubcomplete accesshttps://www.eurobioimaging.eu/nodes/dutch-high-field-imaging-hub
2.15x+2.46x_HF MRINLHigh Field Magnetic Resonance Imaging (human)Human high field Magnetic resonance imaging (MRI), comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level. MRI scanners at high magnetic fields have the advantage to significantly increase the sensitivity and thus improve the image resolution and scan duration. This service is provided on a 9.4T scanner.EURO-BIOIMAGING Dutch High Field MR Hubcomplete accesshttps://www.eurobioimaging.eu/nodes/dutch-high-field-imaging-hub
2.15y+2.46yNLHigh Field Magnetic Resonance Imaging (human)Human high field Magnetic resonance imaging (MRI), comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level. MRI scanners at high magnetic fields have the advantage to significantly increase the sensitivity and thus improve the image resolution and scan duration. This service is provided on a 7T scanner.EURO-BIOIMAGING Dutch High Field MR Hubcomplete accesshttps://www.eurobioimaging.eu/nodes/dutch-high-field-imaging-hub
2.15z+2.46z_MRINLPreclinical Magnetic Resonance ImagingMagnetic resonance imaging (MRI) of animal models, comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level. Used to monitor disease development and to test the efficacy of therapeutic treatments, to develop novel diagnostic tools etc. The service includes animal model preparation, provision of contrast agents, image data processing and analysis.EURO-BIOIMAGING Preclinical Imaging CentreHourhttps://www.eurobioimaging.eu/nodes/preclinical-imaging-centre-(prime)---molecular-imaging-dutch-node
2.12s+2.33s+2.40s_NorMICNOAdvanced Light Microscopy and standard Electron MicroscopyAdvanced Light Microscopy Imaging, including confocal, super-resolution microscopy through SIM or single-molecule localisation, functional imaging for protein dynamics and interactions (FRET, FRAP), TIRF, and live-cell imaging and high-speed imaging.EURO-BIOIMAGING Advanced Light Microscopy Node Oslo - NorMIChourhttps://www.eurobioimaging.eu/nodes/normic-oslo---advanced-light-microscopy-node-oslo
2.15aa+2.46aa_NORMOLIMNOPreclinical Magnetic Resonance ImagingMagnetic resonance imaging (MRI) of animal models, comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level. Used to monitor disease development and to test the efficacy of therapeutic treatments, to develop novel diagnostic tools etc. The service includes animal model preparation, provision of contrast agents, image data processing and analysis.EURO-BIOIMAGING NORMOLIM - Norwegian Molecular Imaging Infrastructurehourhttps://www.eurobioimaging.eu/nodes/normolim,-norwegian-molecular-imaging-infrastructure
2.15a+2.46a_InveonATPreclinical PET-CT and SPECT-CT imagingPositron Emission Tomography (PET) and Single Photon Emission Tomography (SPECT) Imaging of animal models, for monitoring function and metabolism. PET and SPECT imaging can be performed as single modality acquisition but can also be used in combination with CT, for precise spatial localization of the PET/SPECT signals within the subject. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. EURO-BIOIMAGING Austrian BioImaginghourhttps://www.eurobioimaging.eu/nodes/austrian-bioimagingcmi
2.15ab+2.46ab_NORMOLIMNOPreclinical PET imagingPositron Emission Tomography (PET) Imaging of animal models, for monitoring function and metabolism. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. EURO-BIOIMAGING NORMOLIM - Norwegian Molecular Imaging Infrastructurehourhttps://www.eurobioimaging.eu/nodes/normolim,-norwegian-molecular-imaging-infrastructure
2.15ac+2.46ac_NORMOLIMNOPreclinical UltraSound imagingUltraSound Imaging of animal models for visualizing and quantifying tumors, allowing staging, monitoring of therapeutic treatments, or guided injection of drugs. The use of targeting microbubbles as US contrast agents allows the visualization and quantification of specific tumor biomarkers as well. The service includes animal model preparation, probes, image data processing and analysis.EURO-BIOIMAGING NORMOLIM - Norwegian Molecular Imaging Infrastructurehourhttps://www.eurobioimaging.eu/nodes/normolim,-norwegian-molecular-imaging-infrastructure
2.12t+2.33t+2.40t_ALMPLAdvanced Light MicroscopyAdvanced Light Microscopy Imaging, including confocal, super-resolution microscopy through SIM, STED or single-molecule localisation, functional imaging for protein dynamics and interactions (FRET, FRAP, FLIM, FCCS), TIRF, microdissection and two-photon microscopy.EURO-BIOIMAGING Advanced Light Microscopy Node Polandhourhttps://www.eurobioimaging.eu/nodes/advanced-light-microscopy-node-poland
2.14r_ALMPLElectron MicroscopyA range of Electron Microscopy techniques are available, including SEM, TEM, volumeEM in the form of serial section TEM, and Eletron tomography. This also includes methods for labelling proteins of interest in EM samplesEURO-BIOIMAGING Advanced Light Microscopy Node PolandHourhttps://www.eurobioimaging.eu/nodes/advanced-light-microscopy-node-poland
2.15ad+2.46ad_MRIPTPreclinical Magnetic Resonance ImagingMagnetic resonance imaging (MRI) of animal models, comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level. Used to monitor disease development and to test the efficacy of therapeutic treatments, to develop novel diagnostic tools etc. The service includes animal model preparation, provision of contrast agents, image data processing and analysis.EURO-BIOIMAGING Brain Imaging Networkhourhttps://www.eurobioimaging.eu/nodes/brain-imaging-network-bin
2.12u+2.33u+2.40u_InstitutoPTAdvanced Light Microscopy and Electron MicroscopyDifferent fluorescence microscopy methods are offered including expertise in project development and image analysis. The offered methods include super-resolution microscopy techniques such as single molecule localisation, STED, SIM, live cell imaging, mesoscopic imaging via lightsheet and optical projection tomography, functional imaging (FRET, FRAP, FLIM, FCCS), intravital microscopy, high-throughput imaging and correlative techniques. This also includes support techniques such as expansion microscopy, tissue clearing and microdissectionEURO-BIOIMAGING Portuguese Platform of BioImaging PPBIhourhttps://www.eurobioimaging.eu/nodes/portuguese-platform-of-bioimaging-ppbi
2.14sSEElectron MicroscopyElectron Microscopy techniques, including SEM, TEM, volumeEM, and Eletron tomography. Volume EM methods available include array tomography, serial section TEM, and FIB-SEM. This also includes support for correlative methods and different sample preparations, such as chemical and cryo-fixation. Different methods for labelling proteins of interest in EM are available. Various cryo-EM methods can also be requestedEURO-BIOIMAGING Swedish National Microscopy Infrastructurehourhttps://www.eurobioimaging.eu/nodes/swedish-national-microscopy-infrastructure-nmi
2.12v+2.33v+2.40v+2.13j+2.41j_NMISEAdvanced Light MicroscopyAdvanced Light Microscopy methods, including super-resolution (SMLM, STED, SIM) and functional imaging (FRET, FLIM, FRAP, FCCS), intravital microscopy, mesoscopic imaging with lightsheet, lattice lightsheet, feedback microscopy, expansion microscopy and other specialised and support methods.EURO-BIOIMAGING Swedish National Microscopy Infrastructurehourhttps://www.eurobioimaging.eu/nodes/swedish-national-microscopy-infrastructure-nmi
2.25a_LIPFRMolecular profiling of a patient-derived xenograft (PDX) model.Generation of molecular profiles (e.g., DNA-seq, RNA-seq, Immunohistochemistry, others) on a PDX model, either provided by the user or selected from the EurOPDX collection. The standard service (1x Unit of Access) involves generating one type of profile on one sample. To perform more complex studies, more units of access must be selected. For instance, for a combined DNA-seq/RNA-seq profiling two Units of access are required. The service can be combined with drug efficacy testing, e.g., to profile PDXs after in vivo treatment.UNITO Institut Curie1 PDX modelhttps://dataportal.europdx.eu/
2.25b_MCCANLMolecular profiling of a patient-derived xenograft (PDX) model.Generation of molecular profiles (e.g., DNA-seq, RNA-seq, Immunohistochemistry, others) on a PDX model, either provided by the user or selected from the EurOPDX collection. The standard service (1x Unit of Access) involves generating one type of profile on one sample. To perform more complex studies, more units of access must be selected. For instance, for a combined DNA-seq/RNA-seq profiling two Units of access are required. The service can be combined with drug efficacy testing, e.g., to profile PDXs after in vivo treatment.UNITO Netherlands Cancer Institute1 PDX modelhttps://dataportal.europdx.eu/
2.30a_LIPFRIn vivo drug efficacy testing on a selected patient-derived xenograft (PDX) model.Performance of an in vivo drug efficacy study on a selected patient-derived xenografts (PDX) model. The standard service (1x Unit of Access) consists in testing a user-provided drug or compound in a 2-arms study, one control arm and one treatment arm, with 6 NSG mice/arm, and includes a prior tolerability study (3 NSG mice/arm). To perform more complex studies, more units of access must be selected. For instance, for a 4 arms study (e.g., vehicle arm; drug A arm; drug B arm; drug A + drug B arm), two units of access are required. The user could select the PDX model on the EurOPDX Data Portal (https://dataportal.europdx.eu/), supported by the Research Infrastructure. The costs for shipment or, in case, for buying commercial drug(s) or compound(s) to be used in the study are not included in the service.UNITO Institut Curie1 PDX modelhttps://dataportal.europdx.eu/
2.30b_MCCANLIn vivo drug efficacy testing on a selected patient-derived xenograft (PDX) model.Performance of an in vivo drug efficacy study on a selected patient-derived xenografts (PDX) model. The standard service (1x Unit of Access) consists in testing a user-provided drug or compound in a 2-arms study, one control arm and one treatment arm, with 6 NSG mice/arm, and includes a prior tolerability study (3 NSG mice/arm). To perform more complex studies, more units of access must be selected. For instance, for a 4 arms study (e.g., vehicle arm; drug A arm; drug B arm; drug A + drug B arm), two units of access are required. The user could select the PDX model on the EurOPDX Data Portal (https://dataportal.europdx.eu/), supported by the Research Infrastructure. The costs for shipment or, in case, for buying commercial drug(s) or compound(s) to be used in the study are not included in the service.UNITO Netherlands Cancer Institute1 PDX modelhttps://dataportal.europdx.eu/
2.31b_MEDINAESAdvanced profiling services including in-vitro efficacy, toxicological, ADME, stability, PK and PD investigations of confirmed hit compounds ADME-TOX studies platform include drug metabolism studies (CYP inhibition (6 isoforms)and CYP induction, drug metabolites identification, metabolic stability (hepatocytes and liver microsomes), plasma protein binding, plasma stability, reactive metabolite assessment),bioanalysis and PK analysis, drug absorption PAMPA, physicochemical profiling ( thermodynamic solubility, chemical and kinetic stability), toxicity ( cytotoxicity, cardiotoxicity, genotoxicity AMES and Micronucleus test, neurotoxicity)EU-OPENSCREEN Fundación Centro de Excelencia en Investigación de
Medicamentos Innovadores en Andalucía
ADME-tox panel of assays (for 10 compounds)https://www.medinadiscovery.com/ https://www.es-openscreen.com/partner/fundacion-medina/
2.31c_SINTEFNOAdvanced profiling services including in-vitro efficacy, toxicological, ADME, stability, PK and PDAdvanced profiling services including in-vitro efficacy, toxicological, ADME, stability, PK and PD investigations of confirmed hit compounds EU-OPENSCREEN SINTEFin-vitro immunotox.https://www.sintef.no
2.32a_USCESHigh-content image-based profiling of compounds and high-throughput microscopy services, including those applied on patient-derived cells The INNOpharma Screening platform has state-of-the-art equipment for screening campaigns with different technological approaches. EU-OPENSCREEN University of Santiago de Compostela1-month project ( HCS compound prof)https://www.usc.gal/en/department/pharmacology-pharmacy-and-pharmaceutical-technology
2.32b_ITMPDEHigh-content image-based profiling of compounds and high-throughput microscopy services, including those applied on patient-derived cells Establisihing assays and screening for viability, proliferation and multi-parametric high-content microscopy readouts and profiling batches of up to 50 compounds.EU-OPENSCREEN Fraunhofer Institute for Translational Medicine and Pharmacologycompound profilinghttps://www.itmp.fraunhofer.de/
2.32c_MEDINAESHigh-content image-based profiling of compounds and high-throughput microscopy services, including those applied on patient-derived cells Fully integrated fluorescence imaging platform and associated software for bio-imaging assays with primary and established cell lines; multiparametric high content imaging with broad range of target-based and phenotype-based assays (kinetic or single endpoint live-cells analysis; broad fluorophore choice for multiplexing); customized project from experimental approach to data analysis. Determination cell toxicity effects, cell functions, cell signaling pathways , cell structures and morphological featuresEU-OPENSCREEN Fundación Centro de Excelencia en Investigación de
Medicamentos Innovadores en Andalucía
compound profiling (for 10 compounds)https://www.medinadiscovery.com/ https://www.es-openscreen.com/partner/fundacion-medina/
2.35_MEDINAESValidation of the binding affinity and kinetics of compounds towards the drug target via biophysical methods, e.g., SPR, ITC, DSFApplication of biophysical methods to determine the binding affinity and binding kinetics of compounds to the selected targetEU-OPENSCREEN Fundación Centro de Excelencia en Investigación de
Medicamentos Innovadores en Andalucía
DSF analysis (for 10 compounds)https://www.medinadiscovery.com/ https://www.es-openscreen.com/partner/fundacion-medina/
2.31a_CIPFESAdvanced profiling services including in-vitro efficacy, toxicological, ADME, stability, PK and PD investigations of confirmed hit compounds Validation assays to support hit identification ranging from cell to molecular biology and adapting to the applicat request. Alternatively, in-vivo assays in wild type mice for 1 candidate + control group could be performed to determine PK studies by LC-MS or toxicological studies by biochemical markers and histology of major organs. Intravenous adminisration, subcutaneous, intraasal, intraperitoneal or oral administration could be performed. EU-OPENSCREEN Centro de Investigación Príncipe Felipe validation assayhttp://www.VicentResearchLab.com
2.29b_MEDINAESConsultation and support in the development of disease-relevant biochemical, pathway-based and cell-based 2D or 3D assays for compound screening or hit validation Expert assessment and technical support in the development and miniaturization of new biochemical and phenotypic assays to be run in high throughput screening platforms for hit discovery and hit validation follow-upEU-OPENSCREEN Fundación Centro de Excelencia en Investigación de
Medicamentos Innovadores en Andalucía
Hour of consulthttps://www.medinadiscovery.com/ https://www.es-openscreen.com/partner/fundacion-medina/
2.29a_CIPFESConsultation and support in the development of disease-relevant biochemical, pathway-based and cell-based 2D or 3D assays for compound screening or hit validation Advise to be given by phone or by email or by TC/VC, in any doubt concerning the stablishment and use of 2D and 3D based phenotypic assays. Homospheroids, heterospheroids or organoids from commercially available cell lines or patient derived. As well as any adequate confirmation assays for hit validation. EU-OPENSCREEN Centro de Investigación Príncipe Felipe hour of consultationhttp://www.VicentResearchLab.com
2.6_I2PC_EMESElectron Microscopy Image Analysis, I2PC, Madrid, Spain - canSERVThis platform will provide personalised support to projects requiring specialised processing of either electron or X-ray microscopy images. It will also help developers in integrating their methods into generally accessible analysis workflows. The Instruct Image Processing Centre (I2PC) in Madrid will assign personnel to this analysis, as well as the required computational resources.INSTRUCT-ERIC Instruct Centre ES - Instruct Image Processing Centre1day
2.5+2.22+2.47_InstructCZElectron Microscopy, Brno, Czech Republic - canSERVAccess to state-of-the-art instrumentation for electron cryo-microscopy and user support in sample preparation, data acquition, and data processing. Services include high-throughput single particle analysis (SPA) data collection, cryo-electron tomography data acquisition, cryo-FIB lamella preparation, electron diffraction tomography (micro-ED) data acquisition, data analysis, sample preparation and cryo-EM screening, resin embedding, FIB-SEM tomography.INSTRUCT-ERIC Instruct Centre CZ - CEITEC1 dayhttps://www.ceitec.eu
2.10+2.19_instructFIX-ray Diffraction, Oulu, Finland - canSERVBiocenter Oulu Protein Crystallography core facility offers crystallization using nanoliter dispensers and a collection of commercial and self maintained crystallization screens. Crystallization is followed using the IceBear software and remote access to monitor the experiments can be provided following the visit. Biocenter Oulu Protein Crystallography core facility further offers home-source X-ray generator for macromolecular crystal diffraction experiments.INSTRUCT-ERIC Instruct Centre FI - University of Oulu1 day
2.4+2.39_InstructCZProtein Production, Vestec near Prague, Czech Republic - canSERVThe core facility for protein production provides services covering DNA cloning and DNA plasmid preparation, protein expression in E. coli expression systems and recombinant protein purification. Our services can also include optimization of expression and purification protocols, protein identity and purity testing and determination of protein concentration.INSTRUCT-ERIC Instruct Centre CZ - BIOCEV1 dayhttps://www.biocev.eu
2.45_InstructNLPDB-REDO, Amsterdam, Netherlands - canSERVPDB-REDO is a procedure to optimise crystallographic structure models, providing algorithms that make a fully automated decision making system for refinement, rebuilding and validation. With PDB-REDO you can obtain updated and optimised versions of existing entries of the PDB from our DataBank, or you can optimise your own structure model using our Server. Due to robust automation, the procedure can be run at scales from individual structures to high-throughput optimization of thousands of structures. If you want to know more, please check https://pdb-redo.eu INSTRUCT-ERIC Instruct Centre NL - Netherlands Cancer Institute1 dayhttps://www.nki.nl/research/facilities-platforms/protein-facility/
2.7+2.19+2.23+2.37_InstructCZSolution NMR, Brno, Czech Republic - canSERVThe Core Facility of High Field NMR Spectroscopy at CEITEC Instruct centre provides access to NMR spectrometers in the range of proton frequencies from 500 MHz to 950 MHz. The equipment is suited mainly to the studies of structure, dynamics and interactions of biomolecules, i.e. proteins, nucleic acids and carbohydrates. However, the instrumentation is flexible enough to cover various research needs in material science, organic and inorganic chemistry, biochemistry, biology and biophysics.INSTRUCT-ERIC Instruct Centre CZ - CEITEC1 dayhttps://www.ceitec.eu
2.3+2.21+2.38_InstructBENanobody Services, Brussels, Belgium - canSERVThis service includes the generation and characterisation of conformational nanobodies (to facilitate the structural analysis of proteins that are notoriously difficult to purify, to crystallize or to study by another method), as well as rapid selection of nanobodies by screening/affinity maturation. Nanobodies can also be functionally expressed as intrabodies in eukaryotic cells, therefore these single-domain antibodies can also be used to track their targets inside a living cell.INSTRUCT-ERIC Instruct Centre BE - Nanobodies4Instruct1 dayhttps://www.steyaertlab.be
2.8+2.20+2.23+2.35_InstructCZNative Mass Spectrometry and Molecular Biophysics, Vestec near Prague, Czech Republic - canSERVServices include various methodologies for the characterisation of protein structures, identification and quantification of proteins, precise determination of protein molecular mass, characterisation of post translational modifications, analysis of kinetic and thermodynamic parameters of biomolecular interactions, and biophysical characterisation of the structure, function and stability of biological macromolecules like proteins, nucleic acids, lipids and their complexes. The service provided includes data processing and reporting ready for publication.INSTRUCT-ERIC Instruct Centre CZ - BIOCEV1 dayhttps://www.biocev.eu
2.9+2.36_InstructFRCrystalDirect Automated Fragment Screening Pipeline, EMBL, Grenoble - canSERVEMBL Grenoble offers access to a fully automated protein to structure pipeline based on the CrystalDirect technology and the Crystallization Information Management System (CRIMS) software. The CrystalDirect technology enables automated crystal soaking making it possible to support automated, large scale small molecule and Fragment Screening through crystallography efficiently and in a short time.INSTRUCT-ERIC Instruct Centre EMBL - EMBL Grenoble1 daywww.embl.fr/services/index.html
*NEW 2.15f + 2.46f_VEVO US_aCZPreclinical Photocoustic imagingPhotoacoustic Imaging (PAI) of animal models, combining the advantages of optical and US imaging technologies. The US signal makes it possible to achieve an increase of both spatial resolution (micrometric) and penetration depth with respect to optical imaging and reduces scattering phenomena. In comparison with US a wider range of molecules can be used as PAI contrast agents.
PAI allows to get 3D-high resolution images of soft tissues which provide anatomical, functional, and molecular information. The service includes animal model preparation, probes and image data processing and analysis.
EURO-BIOIMAGING CAPIhourhttps://www.eurobioimaging.eu/nodes/center-for-advanced-preclinical-imaging-capi
*NEW 2.17b + 2.15i + 2.46i_SPECT/CT_aFIIntravital microscopyA range of microscopy modalities used for imaging of living animal models, for the real-time visualization of cellular processes in their native environment in the living organism. It can be performed as repetitive imaging on the same animal, allowing biological processes to be tracked over long periods of time, or as acute experiments.
Intravital microscopy can be used e.g. for the monitoring of the migration and cell-cell or cell-matrix interactions of invasive/metastatic tumor cells in different tissues.
EURO-BIOIMAGING FiBIcomplete accesshttps://www.eurobioimaging.eu/nodes/finnish-biomedical-imaging-node
*NEW 2.17c_SCANOMED_aHUPreclinical PET-MRI imagingPositron Emission Tomography (PET) and Single Photon Emission Tomography (SPECT) Imaging of animal models, for monitoring function and metabolism. PET and SPECT imaging can be performed as single modality acquisition but can also be used in combination with CT, for precise spatial localization of the PET/SPECT signals within the subject. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. EURO-BIOIMAGING Medical&Preclinical HUcomplete accesshttps://www.eurobioimaging.eu/nodes/medical-and-preclinical-imaging-hungary
*NEW 2.15k + 2.46k + 2.17d_Semmelweis_aHUPreclinical PET-MRI imagingPositron Emission Tomography (PET) and Single Photon Emission Tomography (SPECT) Imaging of animal models, for monitoring function and metabolism. PET and SPECT imaging can be performed as single modality acquisition but can also be used in combination with CT, for precise spatial localization of the PET/SPECT signals within the subject. The service includes radiotracer provision, animal model preparation, image data processing and analysis. Suitable radioisotopes and tracers can be selected based on the processes/targets that need to be visualized. EURO-BIOIMAGING Medical&Preclinical HUcomplete accesshttps://www.eurobioimaging.eu/nodes/medical-and-preclinical-imaging-hungary
*NEW 2.15k + 2.46k + 2.17d_Semmelweis_bHUPreclinical Magnetic Resonance ImagingMagnetic resonance imaging (MRI) of animal models, comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level.
Used to monitor disease development and to test the efficacy of therapeutic treatments, to develop novel diagnostic tools etc. Several quantitative MR imaging biomarkers can be used to monitor disease progression over time.
The service includes animal model preparation, provision of contrast agents, image data processing and analysis.
EURO-BIOIMAGING Medical&Preclinical HUcomplete accesshttps://www.eurobioimaging.eu/nodes/medical-and-preclinical-imaging-hungary
*NEW 2.15k + 2.46k + 2.17d_Semmelweis_cHUPreclinical UltraSound ImagingUltraSound Imaging of animal models for visualizing and quantifying tumors, allowing staging, monitoring of therapeutic treatments, or guided injection of drugs. The use of targeting microbubbles as US contrast agents allows the visualization and quantification of specific tumor biomarkers as well. The service includes animal model preparation, probes, image data processing and analysis.EURO-BIOIMAGING Medical&Preclinical HUcomplete accesshttps://www.eurobioimaging.eu/nodes/medical-and-preclinical-imaging-hungary
*NEW 2.15k + 2.46k + 2.17d_Semmelweis_dHUPreclinical X-Rays Computed TomographyX-rays Computed Tomography (CT) on tissues, organs or living animal models. CT contrast agents (usually based on Iodine, Barium, Gold) can be used to enhance the contrast. Preclinical microCT systems can be stand alone or integrated with other modalities like optical or nuclear medicine imaging in order to perform multimodal imaging. The service includes animal model prepartion, contrast agents if needed, image data processing and analysis.EURO-BIOIMAGING Medical&Preclinical HUcomplete accesshttps://www.eurobioimaging.eu/nodes/medical-and-preclinical-imaging-hungary
*NEW 2.15q + 2.46q_micro PET_aITPreclinical Magnetic Resonance ImagingMagnetic resonance imaging (MRI) of animal models, comprising a large number of techniques for various applications ranging from simple morphological measurements to the evaluation of molecular processes at the cellular level.
Used to monitor disease development and to test the efficacy of therapeutic treatments, to develop novel diagnostic tools etc. Several quantitative MR imaging biomarkers can be used to monitor disease progression over time.
The service includes animal model preparation, provision of contrast agents, image data processing and analysis.
EURO-BIOIMAGING Neuromedcomplete accesshttps://www.eurobioimaging.eu/nodes/digital-imaging-multimodal-platform-neuromed---dimp-neuromed
*NEW 2.15ad + 2.46ad_MRI_aPTHuman Magnetic Resonance ImagingHuman Magnetic resonance imaging (MRI). The service comprises support for subjects' enrolment and management of ethical requirements. At BIN, MRI can be used either as a single modality or paired to PET.EURO-BIOIMAGING Brain Imaging Networkhourhttps://www.eurobioimaging.eu/nodes/brain-imaging-network-bin
*NEW2.12k + 2.33k + 2.40k_Semmelweis_1HUAtomic Force MicroscopyAtomic Force Microscopy (AFM) is a technique allowing the characterization of sample morphology at nanoscale. Images are acquired by raster scanning a nanometric tip in gentle contact or intermittent contact with the sample. The tip is positioned at the end of a micrometric force transducer, the AFM cantilever: it records the variation of sample topography due to changes of tip-sample interaction during scanning operation. In addition, by measuring the tip-sample interaction force as a function of the tip-sample distance, AFMs can evaluate sample elastic and viscous properties.EURO-BIOIMAGING Debrecen Universitycomplet accesshttps://www.eurobioimaging.eu/nodes/cellular-imaging-hungary
*NEW2.12s + 2.33s + 2.40s_NorMIC_2NOElectron MicroscopyIn transmission electron microscopy (TEM) the accelerated beam of electrons is transmitted through the sample and collected to form an image. To achieve this the specimen is usually sectioned into ultrathin sections less than 100 nm thick. Different methods for sample preparation are availableEURO-BIOIMAGING University of Oslohourhttps://www.eurobioimaging.eu/nodes/normic-oslo---advanced-light-microscopy-node-oslo
*NEW 2.12u + 2.33u + 2.40u_Instituto_2PTElectron MicroscopyTransmission electron microscopy for high-resolution sample imaging, including immuno-labelling and in-resin labellingEURO-BIOIMAGING Instituto de Biologica Molecular e Celular - IBCMhourhttps://www.eurobioimaging.eu/nodes/portuguese-platform-of-bioimaging-ppbi
*NEW 2.5 + 2.22 + 2.47_Instruct_EMBL_HDDEElectron Microscopy and Cryo-CLEM, Heidelberg, EMBL - canSERVThe Imaging Centre at EMBL Heidelberg gives access to state-of-the-art cryo-EM equipment for structure determination projects using the latest technology and methods in single-particle analysis, cryo-electron tomography (cryo-ET) and Cryo-correlative light and electron microscopy (Cryo-CLEM).INSTRUCT-ERICInstruct Centre EMBL - EMBL Heidelberg1 daywww.embl-hamburg.de/services/index.html
*NEW 2.5 + 2.22 + 2.47_Instruct_UHFIElectron Microscopy, Helsinki, Finland - canSERVHigh-resolution cryoEM imaging of protein complexes to study their structure, assembly and function. Access is especially suitable for those wanting to screen samples and get small prelimianary datasets that can be used as proof of principle for access to 300 kV machines or for longer data collection. Correlative light microscopy and cryoEM is possible. Work with genetically modified organisms and BSL2 samples possible (please contact facility in advance). We accept both visits and shipped samples. Biomolecular complexes studied by cryoEM can be purified in the Biocomplex purification facility located in the same building.INSTRUCT-ERICInstruct Centre FI - University of Helsinki1 daywww.helsinki.fi/en
*NEW 2.5 + 2.22 + 2.47_Instruct_FR1FRElectron Microscopy, Strasbourg, France - canSERVThe center offers access for three-dimensional structure determination using cryo-electron microscopy (cryo-EM). The centre has expertise in all aspects of single particle analysis from the automated acquisition of large data sets to image analysis.INSTRUCT-ERICInstruct Centre FR1 - IGBMC1 daywww.igbmc.fr
*NEW 2.5 + 2.22 + 2.47_Instruct_FR2FRElectron Microscopy, Grenoble, France - canSERVServices include quality control negative staining experiments prior to setting up cryo conditions; Data acquisition (single particle imaging and single axis tomography); Cellular EM services, such as plastic embbeding, tokuyasu method, high pressure freezing, immuno-labelling, ultra thin sectionning at room temperature and cryo. The slices can then be observed using a T12 or F20 electron microscope.INSTRUCT-ERICInstruct Centre FR2 - IBS-ISBG1 daywww.ibs.fr/?lang=en
*NEW2.5 + 2.22 + 2.47_Instruct_NECENNLElectron Microscopy, NeCEN, Leiden, The Netherlands - canSERVNeCEN is an open access facility for high resolution cryo-electron microscopy of biological samples. Two state-of-the-art Titan Krios transmission electron microscopes allow efficient and automated high resolution data collection in its two most broadly used forms, single particle analysis (SPA) and cryo-electron tomography (cryo-ET). These imaging techniques allow visualisation of biomolecular structures, such as proteins, macromolecular complexes, bacteria, and cell organelles at subnanometer resolution in close-to-native conditions. The facility is equipped with a BSL2 lab and microscope rooms.INSTRUCT-ERICInstruct Centre NL - NeCEN LU1 daywww.necen.nl
*NEW 2.5 + 2.22 + 2.47_Instruct_DiamondUKElectron Microscopy, Diamond Light Source, Harwell, UK - canSERVThe Electron Bio-Imaging Centre (eBIC) provides scientists with state-of-the-art experimental equipment and expertise in the field of cryo-electron microscopy, for both single particle analysis and cryo-tomography. Currently eBIC houses five Titan Krios microscopes, a Talos Arctica, Glacios, and a Scios and Aquilos cryo-FIB/SEM.INSTRUCT-ERICInstruct Centre UK - Diamond Light Source1 daywww.diamond.ac.uk
*NEW2.5 + 2.22 + 2.47_Instruct_ABSLUKElectron Microscopy, Leeds, UK - canSERVTransmission electron microscopy of biological specimens, from small macromolecular complexes to cells, tissues and organisms. We have two state-of-the-art Titan Krios microscopes equipped with direct electron detectors (DED) for high resolution imaging. Both instruments have AFIS and Fringe free imaging.INSTRUCT-ERICInstruct Centre UK - Astbury Biostructure Laboratory1 daywww.astbury.leeds.ac.uk
*NEW2.5+2.22 + 2.47_Instruct_OPICUKElectron Microscopy, Oxford, UK - canSERVAccess to state-of-the-art equipment for cryo-EM high-resolution single particle analysis, in-situ FIB-SEM and cryo-ET of biological specimens, from small macromolecular complexes to cells. Our facility includes equipment for grid preparation and optimisation (plunging devices and grid micro-patterning) and data collection (Titan Krios G3i, Aquilos 2). Equipment is located within biosafety containment laboratories (ACDP category 3 and DEFRA 4).INSTRUCT-ERICInstruct Centre UK - Oxford Particle Imaging Centre1 daywww.opic.ox.ac.uk
*NEW 2.9 + 2.36_Instruct_DiamondUKXChem Fragment Screening, Diamond Light Source, Harwell, UK - canSERVFragment screening through Macromolecular Crystallography at Diamond’s XChem facility at beamline I04-1, supporting 1000+ compound screensINSTRUCT-ERICInstruct Centre UK - Diamond Light Source1 daywww.diamond.ac.uk
*NEW2.8 + 2.20 + 2.23 + 2.35_Instruct_ULiegeBERobotein Automated Biomolecular Analysis, Liege, Belgium - canSERVNumerous high-throughput (HT) assays are offered, such as HT biophysical characterisation (conformational stability (△G0) of proteins in the presence of chaotropic agents (e.g. urea, guanidinium chloride)); HT binding affinity measurements (enables real-time and label-free determination of binding affinity); HT protein quantitation form crude extracts (fast and accurate protein quantitation from crude extracts (cell extracts, culture media, etc.), with no required purification steps; particularly suitable to measure the concentration of full-length antibodies or various antibody fragments); HT enzymatic assays; HT screen of pH/buffer components (Protein formulation); HT screen of protein refolding conditions from inclusion bodies; Plasmaprofiling for metabolomicsINSTRUCT-ERICInstruct Centre BE - Robotein for Instruct1 dayhttp://www.robotein.ulg.ac.be/
*NEW 2. 2.8 + 2.20 + 2.23 + 2.35_3.2_Instruct_CEITECCZProteomic Mass Spectrometry and Molecular Biophysics, Brno, Czech Republic - canSERVComplete mass spectrometry-assisted proteomics services from sample preparation/purification to qualitative/quantitative characterisation of proteins and their modifications by mass spectrometry and data processing. Services also comprise biophysical characterization of (bio)molecules and the study of biomolecular interactions (determination of affinity, kinetic and thermodynamic parameters). Techniques include analytical ultracentrifugation (AUC), isothermal titration calorimetry (ITC), surface plasmon resonance (SPR), bio-layer interferometry (BLI), microscale thermophoresis (MST), dynamic light scattering (DLS), differential scanning calorimetry (DSC), differential scanning fluorimetry (DSF), and analytical size-exclusion chromatography (SEC-MALS).INSTRUCT-ERICInstruct Centre CZ - CEITEC1 daywww.ceitec.eu
*NEW2. 2.8 + 2.20 + 2.23 + 2.35_3.2_Instruct_UEFFINative Mass Spectrometry, Joensuu, Finland - canSERVUEF core facility offers two state-of-the-art mass spectrometers for versatile utilization of native mass spectrometry for studies of protein folding and assembly of biological macromolecules as well as for quantitative biological interaction studies with large dynamic range.INSTRUCT-ERICInstruct Centre FI - University of Eastern Finland1 dayhttps://instruct-eric.org/centre/university-of-eastern-finland
*NEW. 2.8 + 2.20 + 2.23 + 2.35_3.2_Instruct_UHFISingle-cell Proteomics (SCoPE-MS), Helsinki, Finland - canSERVSCoPE-MS offers a revolutionary technique enabling global level identification and quantification of single cell proteome (>1000 proteins) using mass spectrometry, exceeding the by >ten-fold the identification capability of fluorescence or mass cytometry. Single cell technologies on DNA and RNA level have recently become extremely popular, the SCoPE-MS will extend this to protein/proteome level.INSTRUCT-ERICInstruct Centre FI - University of Helsinki1 daywww.helsinki.fi/en
2. 2.8 + 2.20 + 2.23 + 2.35_3.2_Instruct_UUNLMass Spectrometry, Utrecht, Netherlands - canSERVMass spectrometry based proteomics enables the identification and quantification of cellular proteins and their modified forms. The Biomolecular Mass Spectrometry and Proteomics group at the Bijvoet Centre provides access to qualitative proteomics analysis of proteins in complex samples. Instrumentation available includes advanced (2D)-HPLC and state-of-the-art mass spectrometers such as Thermo Scientific Q-Exactive Orbitraps.INSTRUCT-ERICInstruct Centre NL - Bijvoet Centre1 dayhttps://www.uu.nl/en/research/bijvoet-centre-for-biomolecular-research
2.8 + 2.20 + 2.23 + 2.35_Instruct_ICSASSKMass Spectrometry - Glycan Analysis, Bratislava, Slovakia - canSERVGlycan structure analysis by applying spectroscopic methods, such as Protein Mass spectrometry using an Orbitrap Elite mass spectrometer, coupled with HPLC/nanoLC or Native Mass Spectrometry using an UltrafleXtreme II MALDI mass spectrometer, coupled with nanoLCINSTRUCT-ERICInstruct Research Site SK - ICSAS1 daywww.sav.sk/?lang=en
2. 2.8 + 2.20 + 2.23 + 2.35_3.2_Instruct_ABSLUKMass Spectrometry, Leeds, UK - canSERVServices include hydrogen/deuterium exchange (HDX)-MS analyses to determine protein folding states, effects of ligand binding and protein interactions (e.g. epitope mapping); Covalent labelling setup for hydroxyl radical foot printing via Fast Photochemical Oxidation of Proteins (FPOP) linked to mass spectrometry, to probe changes in solvent accessibility; High-resolution native MS (for heterogeneous systems at high mass-to-charge ratios, e.g. membrane protein lipid complexes); Ion mobility capabilities INSTRUCT-ERICInstruct Centre UK - Astbury Biostructure Laboratory1 daywww.astbury.leeds.ac.uk
1. 2.7 + 2.19 + 2.23 + 2.37_3.1_Instruct_GUFDESolution NMR, Frankfurt, Germany - canSERVThis service provides access to NMR spectrometers in the range of proton frequencies from 600 MHz to 950 MHz to elucidate the structure and funtional mechanism of biomolecules, such as proteins, RNA, ligand-protein and ligand-RNA complexes. INSTRUCT-ERICGoethe University Frankfurt1 day
1. 2.7 + 2.19 + 2.23 + 2.37_3.1_Instruct_UHFINMR, Helsinki, Finland - canSERVThe NMR facility provides services on the field of liquid NMR. The main focus area includes studies of structure and dynamics of biomacromolecules, but also other types of analyses are very well possible e.g. with broadband probe on 600 MHz.INSTRUCT-ERICInstruct Centre FI - University of Helsinki1 daywww.helsinki.fi/en
1. 2.7 + 2.19 + 2.23 + 2.37_3.1_Instruct_FR2FRNMR, Grenoble, France - canSERVAccess to solid state NMR equipment with state-of-the-art magic-angle spinning (MAS) capabilities, and triple-resonance probes for different rotor sizes and spinning speeds. High-sensitivity solution-state NMR applications are also offered using instruments with Bruker electronics (Avance III HD) and cryogenically cooled probes. Standard Bruker experiment libraries and additional in-house libraries for optimised fast NMR data acquisition (e.g. SOFAST, BEST, HADAMAC-type experiments) are available. Fast-mixing equipments required for real-time studies of kinetic processes, e.g. protein folding are also available.INSTRUCT-ERICInstruct Centre FR2 - IBS-ISBG1 daywww.ibs.fr/?lang=en
1. 2.7 + 2.19 + 2.23 + 2.37_3.1_Instruct_CERMITNMR, Florence, Italy - canSERVSolid-state and solution NMR for structure and dynamic characterisation of biological macromolecules and their complexes; “protonless” NMR experiments and in-cell NMR spectroscopy, tailored pulse sequences for structural determination of paramagnetic systems; Relaxometry (structural and dynamical information on nuclear spin systems); Electron Paramagnetic Resonance (EPR)-based methods to map local dynamic and structural features of biomolecules, to explore different modes of biomolecule-ligand interaction, to obtain long-range structural restraints and to probe metal-ion-binding sites.INSTRUCT-ERICInstruct Centre IT - CERM/CIRMMP1 daywww.cerm.unifi.it
1. 2.7 + 2.19 + 2.23 + 2.37_3.1_Instruct_UUNLNMR, Utrecht, Netherlands - canSERVSolid state and solution NMR equipment for the characterisation of biomolecules, their structure determination and dynamical and functional studies. Services also include structural determination of high molecular weight proteins or complexes, such as nucleosome-protein complexes (200-250 kDa) and other special applications, such as in-cell NMR experiments. Support for applications involving interactions of intrinsically disordered proteins as well as support for medium to high-througput screening.INSTRUCT-ERICInstruct Centre NL - Bijvoet Centre - UU1 dayhttps://www.uu.nl/en/research/bijvoet-centre-for-biomolecular-research
1. 2.7 + 2.19 + 2.23 + 2.37_3.1_Instruct_ABSLUKSolution NMR, Leeds, UK - canSERVThis service offeres solution NMR based on direct heteronuclear detection (15N and 13C, 5mm TXO Cryoprobe). Combination of high field and Carbon detection for complex or large intrinsically disordered proteins and characterisation of protein sidechains. Techniques using 15N detection are under development and are of interest in chemically exchanging systems, as an alternative to Ca detection and when deuteration is not feasible. Solution NMR services for mass limited samples and high ionic strength samples using a smaller diameter probe (3mm TCI Cryoprobe) for optimised sensitivity.INSTRUCT-ERICInstruct Centre UK - Astbury Biostructure Laboratory1 daywww.astbury.leeds.ac.uk
2.4 + 2.39_Instruct_UHFIBiomolecular Complex Purification (Biocomplex), Helsinki, Finland - canSERVSample preparation and purification for functional and structural studies using preparative ultracentrifugation, monolithic chromatography and asymmetrical flow field-flow fractionation technologies. These can be used in different combinations to purify large macromolecular complexes such as viruses, exosomes, membrane vesicles, large protein complexes, and ribonucleoprotein complexes. Samples produced at Biocomplex can be further studied at the CryoEM facility located in the same building. We accept both visits and shipped samples.INSTRUCT-ERICInstruct Centre FI - University of Helsinki1 daywww.helsinki.fi/en
2.4 + 2.39_Instruct_FR1FRProtein Production and Gene Tagging, Strasbourg, France - canSERVRecombinant expression of protein and multi-protein complexes in prokaryotic and eukaryotic hosts as well as biomass production for isolation of poorly abundant proteins and endogenous complexes; Gene tagging with the CRISPR-Cas9 system for introduction of affinity tags to facilitate the purification of endogenous complexes or for tagging proteins with fluorescent reporters in view of imaging and functional proteomics applications, in the frame of a partnership with the TacGene platform from the Museum d’Histoire Naturelle (Paris, France).INSTRUCT-ERICInstruct Centre FR1 - IGBMC1 daywww.igbmc.fr
2.4 + 2.39_Instruct_WISILProtein Production, Weizmann Istitute, Israel - canSERVProtein production in bacteria, baculovirus, and mammalian cells. Proteins are typically purified following a standard 3 step protocol which includes: affinity capture, ion exchange and size exclusion. Specific techniques can be employed depending on the protein, the experimental requirements, tags and yields. Removal of tags with TEV protease can be employed.INSTRUCT-ERICInstruct Centre IL - Weizmann Institute of Science1 dayhttp://www.weizmann.ac.il/ISPC/
2.4 + 2.39_Instruct_NKINLProtein Production in Insect and Mammalian Cells, Amsterdam, Netherlands - canSERVProtein production using insect and mammalian cells, and assisting users with the design of their expression construct in terms of solubility, domain boundaries, etc. We offer a variety of expression vectors, including a set of LIC vectors (Ligand Independent Cloning). For expression of intracellular and extracellular proteins in insect cells we have SF9 and SF21 systems. Our mammalian expression system has been optimized for HEK-293T cells, and we also have the glycosylation free, gnT1-deficient HEK293S variety. Protein expression in bacteria (E. coli) is beyond the scope of the facility.INSTRUCT-ERICInstruct Centre NL - Netherlands Cancer Institute1 dayhttps://www.nki.nl/research/facilities-platforms/protein-facility/
2.4 + 2.39_Instruct_EMBL_HHDEMolecular Biophysics, Hamburg, Germany - canSERVServices for protein quality control in addition to biophysical characterization techniques for studying the interaction of biomolecules, including MALDI (MS), Thermofluor (TF), Circular Dichroism (CD), Fourier-transform infrared spectroscopy (FTIR), Isothermal Titration Calorimetry (ITC), nano Differential Scanning Fluorimetry (nDSF), Surface Plasmon Resonance (SPR), Biolayer interferometry (Octet), Microscale Thermophoresis (MST), Dynamic Light Scattering (DLS), Mass Photometry (Refeyn), Ligand screening kinetics (Creoptix-WAVE) and time resolved reactions (stopped-flow).INSTRUCT-ERICInstruct Centre EMBL - EMBL Hamburg1 daywww.embl-hamburg.de/services/index.html
2.10 + 2.19_Instruct_BIOCEVCZX-ray Diffraction and Bio-SAXS, Vestec near Prague, Czech Republic - canSERVCrystallization of biomacromolecules and their complexes as well as services for characterization of protein structures using single crystal X-ray diffraction and small-angle X ray scattering for analysis of liquid samples. The quality of high-intense X-ray beams is ensured by using of MetalJet sources (Excillum). The provided services include assistance with data processing and reporting ready for publication.INSTRUCT-ERICInstruct Centre CZ - BIOCEV1 daywww.biocev.eu
2.10 + 2.19_Instruct_EMBL_HHDEX-ray Diffraction and Bio-SAXS, Hamburg, Germany - canSERVMacromolecular crystallography and biological small-angle X-ray scattering (SAXS). SAXS allows one to study the overall (quaternary) structure and structural transitions in a non-restrictive sample environment, directly in solution. Examples of services offered are: (i) combinatorial high throughput ligands/additives screening; (ii) studies of equilibrium mixtures and membrane proteins with online SEC-SAXS coupled with biophysical analysis, and (iii) time-resolved studies of processes, from sub-ms ((un)folding) to hours (oligomerization, fibrillation). Sample preparation and characterisation (crystallization screens suitable for soluble and membrane proteins), processing and evaluation of the data.INSTRUCT-ERICInstruct Centre EMBL - EMBL Hamburg1 daywww.embl-hamburg.de/services/index.html
2.18l_IRST IRCCS_bITRNA sequencingtotal RNA or mRNA sequencing in tumoral and/ or non tumoral samples using NGS technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) IRST IRCSS samplewww.irst.emr.it
2.18j_CNAG_bESRNA sequencingtotal RNA or mRNA sequencing in tumoral and/ or non tumoral samples using NGS technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) CNAG1Gb sequence datahttps://www.cnag.crg.eu/
2.18f_UL Biobank_aPLWhole Genome SequencingWhole genome sequencing in tumoral and non tumoral samples using next generation sequencing technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) Biobank Lab of the University of Lodzsamplewww.biobank.uni.lodz.pl
2.18f_UL Biobank_bPLRNA sequencingRNA sequencing in tumoral/ non tumoral samples using next generation sequencing technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) Biobank Lab of the University of Lodzsamplewww.biobank.uni.lodz.pl
2.18f_UL Biobank_cPLWhole Exome SequencingWhole exome sequencing in tumoral and non tumoral samples using next generation sequencing technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) Biobank Lab of the University of Lodzsamplewww.biobank.uni.lodz.pl
2.18f_UL Biobank_dPL16S rRNA microbiome sequencingMicrobial profiling by sequencing 16S ribosomal RNA (rRNA) with next generation sequencing technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) Biobank Lab of the University of Lodzsamplewww.biobank.uni.lodz.pl
2.18f_UL Biobank_ePLMetagenomic microbiome sequencingMicrobial profiling by shotgun metagenomics using next generation sequencing technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) Biobank Lab of the University of Lodzsamplewww.biobank.uni.lodz.pl
*NEW 2.18k_UNIMIB Med_aITExome sequencingWhole exome sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) UNIMIB_MEDcellhttps://en.unimib.it/
*NEW 2.18k_UNIMIB Med_bITTarget DNA sequencing: custom gene panelsTarget sequencing of custom gene panels in tumoral and/ or non tumoral samples using next generation sequencing technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) UNIMIB_MEDcellhttps://en.unimib.it/
*NEW2.18k_UNIMIB Med_cITRNA sequencingtotal RNA or mRNA sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) UNIMIB_MEDcellhttps://en.unimib.it/
2.18l_IRST IRCCS_cITWhole exome sequencingWhole exome sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) IRST IRCSS samplewww.irst.emr.it
2.18l_IRST IRCCS_dITTarget DNA sequencing: custom gene panelsTarget sequencing of custom gene panels in tumoral and/ or non tumoral samples using next generation sequencing technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) IRST IRCSS samplewww.irst.emr.it
2.18j_CNAG_cESWhole exome sequencingWhole exome sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) CNAG - CRG1Gb sequence datahttps://www.cnag.crg.eu/
2.18j_CNAG_dESWhole genome enzymatic methyl sequencing Whole genome enzymatic methyl sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) CNAG - CRG1Gb sequence datahttps://www.cnag.crg.eu/
2.18j_CNAG_eESSingle cell ATAC sequencingSingle cell ATAC (assay for transposase-accessible chromatin) sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) CNAG - CRG1Gb sequence datahttps://www.cnag.crg.eu/
2.18l_IRST IRCCS_eITsmallRNA sequencingsmallRNA sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) IRST IRCSS samplewww.irst.emr.it
2.18l_IRST IRCCS_fIT Single cell mRNA sequencingSingle cell mRNA sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) IRST IRCSS samplewww.irst.emr.it
2.18l_IRST IRCCS_gITSpatial transcriptomicsSpatial transcriptomics in tumoral and/ or non tumoral samples using next generation sequencing technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) IRST IRCSS samplewww.irst.emr.it
2.18j_CNAG_fESSingle cell mRNA sequencingSingle cell mRNA sequencing in tumoral and/ or non tumoral samples using next generation sequencing technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) CNAG - CRG1Gb sequence datahttps://www.cnag.crg.eu/
2.18j_CNAG_gESSingle cell immune repertoire profilingSingle cell immune repertoire profiling (TCR/ BCR) in tumoral and/ or non tumoral samples using next generation sequencing technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) CNAG - CRG1Gb sequence datahttps://www.cnag.crg.eu/
2.18j_CNAG_hESSpatial transcriptomicsSpatial transcriptomics in tumoral and/ or non tumoral samples using next generation sequencing technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) CNAG - CRG1Gb sequence datahttps://www.cnag.crg.eu/
2.18u_Single cell _LIED_bDESingle cell immune repertoire profilingSingle cell immune repertoire profiling (TCR/ BCR) in tumoral and/ or non tumoral samples using next generation sequencing technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) LIED, University of Lubeck sample
2.18u_Single cell _LIED_cDESpatial transcriptomicsSpatial transcriptomics in tumoral and/ or non tumoral samples using next generation sequencing technologiesBIOBANKS AND BIOMOLECULAR RESOURCES RESEARCH INFRASTRUCTURE CONSORTIUM (BBMRI-ERIC) LIED, University of Lubeck sample

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